Anti-acantamoeba activity of chlorhexidine and hydrogen peroxide

Davies, D. J. G.; Anthony, Yolande; Meakin, B. J.; Kilvington, Simon; White, D. G.

doi:10.1016/s0141-7037(88)80028-4

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Log phase axenic cultures of trophozoites (incubated at 30°C on a gyratory shaker set at 100 rpm) were centrifuged at 740 × g for 5 minutes and then handled according to the method of Davies et al 12. Incubation in encystment medium was at 30°C on a shaker set at 100 rpm for 48 hours.…”

Section: Methodsmentioning

confidence: 99%

“…Experimental findings concerning the efficiency of chemical disinfection systems are inconclusive because the results vary 10-14. This could be due to variation in the susceptibility to contact lens solutions of different species and isolates of Acanthamoeba 10 12 15 or to the different experimental procedures which have been employed 1213 Standardised testing of the efficacy of contact lens disinfection systems against Acanthamoeba is, therefore, needed 16…”

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confidence: 99%

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Anti-Acanthamoeba activity of contact lens solutions

Niszl

Markus

1998

British Journal of Ophthalmology

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Aims-This study was undertaken to investigate the eVects of contact lens disinfecting solutions on strains of Acanthamoeba from the United Kingdom and southern Africa and to compare the results with those of other researchers. No information was previously available for southern African isolates. Methods-11 contact lens solutions were tested on cysts of 10 strains of Acanthamoeba. Results-Not all solutions used in the study were eVective, with some for hard and gas permeable contact lenses being more satisfactory than those for soft contact lenses. The most eVective of the gas permeable and hard contact lens solutions tested was Transoak (0.01% (wt/vol) benzalkonium chloride), which killed cysts of all strains within 4 hours of exposure. Oxysept 1 (31 mg hydrogen peroxide/ml) was the best soft contact lens solution tested. It eliminated cysts of certain strains within 4 hours, whereas cysts of other strains were only inactivated within either 8 or 72 hours. Conclusions-Manufacturers should be aware of the killing time for Acanthamoeba by contact lens solutions and should provide appropriate guidelines for the use thereof. The killing time for cysts of the African and UK isolates studied is, in general, similar. Therefore, it must in the present state of knowledge be assumed that usage guidelines suggested in the UK are also appropriate for travellers to South Africa and for local residents in South Africa.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Anti-Acanthamoeba activity of contact lens solutions

Niszl

Markus

1998

British Journal of Ophthalmology

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“…treated with propamidine isethionate (35), the disease frequently necessitates surgical intervention (6,34). Reports on the susceptibility of Acanthamoeba strains to chemotherapeutic agents and contact lens disinfectants often give conflicting results (5,6,9,17,21,35). Strain Ac/PHL/33 of group G is inherently resistant to propamidine isethionate (15), while strain Ac/PHL/23 (SHI) of group A is susceptible (unpublished observations).…”

Section: Fig 5 Differentiation Of Acanthamoeba Strains By Bgii Rflpmentioning

confidence: 99%

Differentiation of Acanthamoeba strains from infected corneas and the environment by using restriction endonuclease digestion of whole-cell DNA

1991

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Restriction endonuclease digestion of Acanthamoeba whole-cell DNA was used to study the relationship between 33 morphologically identical strains from keratitis cases (30 strains), contact lens storage containers (2 strains), and soil (1 strain). Samples digested with BgllI, EcoRI, or Hindlll and separated by agarose gel electrophoresis contained detectable mitochondrial DNA restriction fragment length polymorphisms (RFLPs). By comparing RFLPs, the strains could be assigned to seven multiple-strain and three single-strain groups. The largest of these contained nine strains, eight of which were isolated in keratitis cases in various locations worldwide and may indicate a group particularly associated with keratitis. Restriction endonuclease analysis of whole-cell DNA is proposed as a valuable technique for detecting mitochondrial DNA RFLPs in the differentiation of morphologically identical Acanthamoeba strains and may therefore be useful in resolving the complex taxonomy of the genus, which has hitherto been founded on subjective morphological criteria.

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