Anthropogenic pollution stimulates oxidative stress in soft tissues of mussel Crenomytilus grayanus (Dunker1853)

Abstract

The digestive gland and gills of the mussel Crenomytilus grayanus extracted from three locations -(i) sampled from a clean and (ii) polluted site and (iii) transplanted from the nonpolluted to polluted site -were analysed for antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase), total oxyradical scavenging capacity and levels of lipid peroxidation products (malondialdehyde, conjugated dienes and lipofuscin). Perturbation of redox status was found in both digestive gland and gill tissues of mussels living in the polluted area. As the activities of superoxide dismutase and catalase were 1.2-3 times higher, the total oxyradical scavenging capacity was lower by 20-35% and the levels of lipid peroxidation products were 2-7 times higher compared to mussels from the reference site. In transplanted mussels, the lipid peroxidation process in both tissues was significantly stimulated (the level of conjugated dienes was increased 1.7-2.5-fold; malondialdehyde and lipofuscin contents were increased 3.5-5-fold) and the total oxyradical scavenging capacity fell by 50-70%. In addition, the transplantation generally resulted in transient and variable responses of antioxidant enzymes for both tissues. Complex response-behaviour of the antioxidant enzymes strongly points to the necessity of employing a combined approach that takes into account activities of antioxidant enzymes and the total oxyradical scavenging capacity, as well as measurement of oxidative damage (e.g., lipid peroxidation) to evaluate the physiological health of molluscs.

Effect of seasonality on oxidative stress responses and metal accumulation in soft tissues of Aulacomya atra, a mussel from the South Atlantic Patagonian coast

“…Similarly, our results showed that lipid peroxidation products (TBARS) were preferentially increased in gills and digestive gland of ribbed mussels from the polluted sites (harbor areas). Further evidence of increased oxidative damage to lipids due to metals exposure in mussels were observed in Perna perna in laboratory experiments (de Almeida et al, 2004) and in Crenomytilus grayanus in field studies (Belcheva et al, 2011). In addition, lipid peroxidation products may cause cellular damage by changing cell membrane integrity or by directly attacking proteins (Mattie and Freedman, 2001).…”

Hemolymph cholinesterase activity in the Mussel Crenomytilus grayanus (Dunker, 1853) that was exposed to adverse natural and anthropogenic conditions

“…Over the past 3 decades, Gray's mussel has been widely used in laboratory based and field investigations into bioaccumulation of heavy metals [29,30], organochlorine pesticides [1] and radio nuclides [31], as well as in the study of the mechanism of toxic effects caused by complex pollution [4,11,18]. water circulations, which form mesoscale gyres and upwelling, exists for several days to 2 months during the summer and fall seasons [3,5].…”

Report on the Annual Gametogenesis and Tissue Biochemical Composition in the Gray mussel, Crenomytilus grayanus (Dunker 1853) in the Subtidal Rocky Bottom on the East Coast of Korea

“…Endemic to the coastal East/Japan Sea, C. grayanus has a wide distribution from the Sea of Okhotsk, Kurils, the Peter the Great Bay, and the coastal East/Japan Sea (Min et al 2004;Galysheva 2008;Belcheva et al 2011;Selin and Dulenina 2012;Lutaenko and Noseworthy 2019). Despite their popularity, no studies have investigated the annual gametogenesis of C. grayanus in the endemic area.…”