Anthropogenic and environmental factors associated with high incidence of mcr-1 carriage in humans across China

Shen, Yingbo; Zhou, Hongwei; Jiao, Xu; Wang, Yongqiang; Zhang, Qijing; Walsh, Timothy R.; Shao, Bing; Wu, Congming; Hu, Yanyan; Lü, Yang; Shen, Zhangqi; Wu, Zhongliang; Sun, Qiaoling; Ou, Yanran; Wang, Yueling; Wang, Shaolin; Wu, Yongning; Cai, Chang; Li, Juan; Shen, Jianzhong; Zhang, Rong; Wang, Yan

doi:10.1038/s41564-018-0205-8

Cited by 144 publications

(130 citation statements)

References 32 publications

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“…The high diversity of the mcr-1-positive E. coli and K. pneumoniae isolates detected in the present study corresponds to the results for mcr-positive Enterobacteriaceae isolated from different sources (food, animals, and humans) in other studies (Zurfluh et al, 2016;Luo et al, 2017;Roschanski et al, 2017;Zurfluh et al, 2017;El Garch et al, 2018). However, some studies have described an increased prevalence of E. coli with mcr-1 belonging to ST10 obtained from food animals, food, and humans (Zurfluh et al, 2016;El Garch et al, 2018;Shen et al, 2018;Wu et al, 2018). Similarly, E. coli isolates found here belonging to ST10 (n = 5) and ST744 (n = 5) were most often detected.…”

Section: Discussionsupporting

confidence: 89%

“…The most common type of plasmid was IncX4, which carried the mcr-1 gene in 77% (44/57) of E. coli and K. pneumoniae isolates from turkey meat and liver samples (n = 16) from the Czechia, Germany, Poland, and Brazil. IncX4 plasmids carrying mcr-1 have also been described in E. coli isolated from meat and humans in China Luo et al, 2017;Shen et al, 2018), chicken meat from Brazil (Monte et al, 2017), chicken and turkey meat from the EU (Hasman et al, 2015;Alba et al, 2018), and pigs in Germany (Roschanski et al, 2017). The conjugation assay showed that the majority of IncX4 plasmids carrying mcr-1 were transferable to recipient E. coli cells (14/17).…”

Section: Discussionmentioning

confidence: 99%

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Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

et al. 2019

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The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

et al. 2019

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“…Abuse of the colistin in animal production is believed to drive the selective pressure for the emergence and transmission of mcr‐1 . Moreover, animal products and farming environment are suspected to be important routes to spread the mcr‐1 gene to human beings (Liu et al, ; Shen, Zhou, et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…The emergence of the colistin‐resistant Enterobacteriaceae , such as MCRPEC, deteriorates the situation in the face the public medical “post‐antibiotic era” (Shen, Zhou, et al, ). Epidemiology research of mcr‐1 ‐positive Enterobacteriacea raises serious concerns about the increasing risk to severe humans infections by PDR Gram‐negative pathogens.…”

Section: Introductionmentioning

confidence: 99%

Population structure and antimicrobial resistance traits of avian‐origin mcr‐1 ‐positive Escherichia coli in Eastern China, 2015 to 2017

Zhuge

Tang

et al. 2019

Transbound Emerg Dis

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Recent emergence of mcr-1-positive Escherichia coli (MCRPEC) is causing serious concern around the world. Due to poultry-origin E. coli holding zoonotic potential, the improved understandings of MCRPEC population structure and antimicrobial resistance are critical to public health purposes. This study provided novel insights into the molecular epidemiology of avian-origin MCRPEC. For the mcr genes prevalence study, we analysed 1,360 E. coli recovered from avian colibacillosis in eastern China from 2015 to 2017. The mcr-1 was present in 172 (12.6%) E. coli isolates.For all of MCRPEC isolates, MICs of colistin were ≥4 mg/L. Avian-origin MCRPEC was widely distributed throughout phylogroups A, B1, B2, D, and F. Moreover, those isolates were assigned to 52 unique STs, such as ST48, ST117, ST131, and ST648, suggesting substantial horizontal dissemination of mcr-1 gene through avian-origin E. coli populations. The susceptibility of MCRPEC isolates was tested with 26 antimicrobial agents from 16 antimicrobial categories. There were high resistance rates of MCRPEC isolates against the clinically used antibiotics. All MCRPEC isolates in this study presented the multidrug-resistant (MDR) trait were even considered as extensively drug-resistant (XDR) strains. Resistance genotypes and plasmid replicon profiling showed that a majority of MCRPEC isolates contained plasmid-mediated resistance genes and exhibited the co-existence of mcr-1 with ESBLs and pAmpCs genes. Furthermore, the overlapped distribution of ST types and resistance gene contents was detected among MCRPEC isolates from humans and poultry. Besides mcr-1, our findings highlighted a significant prevalence of plasmid-mediated resistance genes among avian-origin MCRPEC isolates. K E Y W O R D Santimicrobial susceptibility, avian-origin MCRPEC, resistance genotypes, sequence type

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“…Since the mcr-1 gene was reported, many studies clearly clarified the status quo of its transmission and provided its epidemic evidence in food, livestock and human [16–18]. How to effectively avoid the public health hazards induced by mcr-1 has become an important challenge.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive proteomic and metabolomic profiling of mcr-1 mediated colistin resistance in Escherichia coli

Wang

Meng

et al. 2018

Preprint

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19The spread of mcr-1 in human and veterinary medicine has jeopardized the use of 20 polymyxins, the last-resort antibiotics against life-threatening multidrug-resistant 21 Gram-negative bacteria. As a lipid-modified gene, whether mcr-1 brings proteomic and 22 metabolomic changes in the bacteria and affects the corresponding metabolic pathway 23 is largely unknown. Herein, we used label-free quantitative proteomics and untargeted 24 metabolomics to profile comprehensive proteome and metabolome characteristics of 25 mcr-1-mediated colistin-resistant and -sensitive Escherichia coli and further insight the 26 resistant mechanism of colistin. We identified large sets of differential expression 27 proteins and metabolites that contributed to mcr-1-mediated antibiotic resistance 28 predominantly in the different growth conditions with and without colistin. mcr-1 could 29 cause the down-regulated expression of most proteins to adapt drug pressure. Pathway 30 analysis showed that metabolic process was significantly affected, mainly related to 31 glycerophospholipid metabolism, thiamine metabolism, and lipopolysaccharide 32 biosynthesis. The substrate phosphatidylethanolamine for mcr-1 to mediate colistin 33 resistance is accumulated in colistin-resistant E. coli. Notably, mcr-1 can not only cause 34 the phosphoethanolamine modification of bacterial cell membrane lipid A, but also 35 affect the biosynthesis and transport of lipoprotein in colistin resistance through 36 disturbing the expression of efflux pump proteins involved in cationic antibacterial 37 peptide resistance pathway. Overall, the disturbed glycerophospholipid metabolism, 38 lipopolysaccharide biosynthesis and the accumulation of the substrate 39 phosphatidylethanolamine is closely related with mcr-1-mediated colistin resistance 3 40and these findings can further provide valuable information to inhibit colistin resistance 41 by blocking this metabolic process. 43Since the discovery of the plasmid-mediated colistin resistance determinant mcr-1 46 in Enterobacteriace in 2015, its widespread dissemination brings potential threats to the 47 treatment of extensively drug resistant bacterial infections in animals and humans. 48Although many excellent achievements in its epidemic evidence and resistance 49 mechanism have been made, the in-depth colistin resistance mechanisms, such as the 50 omics profile of bacteria mediated by mcr-1 in Escherichia coli needs further research. 51 In this study, comprehensive proteomic and metabolomic profiles of mcr-1-mediated 52 colistin-resistant and -sensitive Escherichia coli were investigated to clarify the protein 53 and metabolite features related to mcr-1. The proteome characteristics of colistin-54 resistant E. coli carrying mcr-1 under different selection pressures of colistin and 55 polymyxin B were investigated, and the metabolic pathways including 56 glycerophospholipid metabolism, thiamine metabolism, and lipopolysaccharide 57 biosynthesis were disturbed. Up to 67 differential metabolites were identified in mcr...

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Anthropogenic and environmental factors associated with high incidence of mcr-1 carriage in humans across China

Cited by 144 publications

References 32 publications

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

Population structure and antimicrobial resistance traits of avian‐origin mcr‐1 ‐positive Escherichia coli in Eastern China, 2015 to 2017

Comprehensive proteomic and metabolomic profiling of mcr-1 mediated colistin resistance in Escherichia coli

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