Anthrone in Estimation of Hexose Sugars with Special Reference to Pentose Interference

Johanson, R.

doi:10.1021/ac60092a020

Cited by 36 publications

(7 citation statements)

References 5 publications

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“…Proteins were measured by the Coomassie brilliant blue G-250 method (Sedmak and Grossberg, 1977) using bovin serum albumin and chymotrypsin (Sigma Chemical Co., U.S.A.) as standards, and by anthrone or glucostate (Sigma) methods (Johanson, 1954) for sugar analysis, using glucose as standard. Fatty acids were detected by gas chromatography (Ruben et al, 1981), and amino acids in an amino acid analyser (Dionex D-502).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Reproducible induction of cavity spot in carrots and physiological and microbial changes occurring during cavity formation

Soroker

Bashan

Okon

1984

Soil Biology and Biochemistry

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Summary-Inoculation of carrots with 40 types of bacteria, both aerobic and anaerobic, including clostridia isolated from cavity spots, failed to induce cavity spot in carrots. A combined stress of minimum 6 h flooding and temperatures higher than 28°C clearly induced cavity formation. Sugars, amino acids, lipids and minerals leaked from the carrots after flooding and heating the roots. A longer growth period following stress markedly increased cavity spots. Soil types (sandy loam and loess) and several carrot cultivars tested had no marked effect on spot formation. Cavities were formed in stressed carrots grown in sterilized soil containing only one type of bacterium, a Gram-negative short rod. Scanning electron micrographs revealed that after carrots were subjected to combined stress, microscopic cavities nearly free of bacteria were formed under the epidermis. Proliferation of bacteria was concommitant with the appearance of visible cavities. Cell-free extracts of infected carrots showed higher protease and pectinase-specific activities, as well as significantly higher peroxidase and polyphenoloxidase activities and total phenol content as compared to healthy carrots.

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Section: Analytical Proceduresmentioning

confidence: 99%

Reproducible induction of cavity spot in carrots and physiological and microbial changes occurring during cavity formation

Soroker

Bashan

Okon

1984

Soil Biology and Biochemistry

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“…(f) Analytical The nitrogen fractions and starch were determined on dried material as described by Turner (1949). Total soluble carbohydrates were determined by the anthrone method as modified by Johanson (1954), using dried material in 1952 and fresh material in 1953.…”

Section: (E) Cell Size Measurementsmentioning

confidence: 99%

Physiology of Pea Fruits I. the Developing Fruit

McKee¹,

Robertson²,

Jb³

1955

Aust. Jnl. Of Bio. Sci.

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Pea fruits from two crops were sampled at different times from flowering. Changes in the fresh weight, dry weight, starch, soluble carbohydrate, protein nitrogen, and soluble nitrogen in both seeds and hulls were followed in two seasons and related Jo the changes in cell volume in the seeds. In one season respiration rates and phosphate, pectin, and ascorbic acid contents were also investigated. The seeds gained more carbohydrate and nitrogen than was lost by the hulls. Starch and protein were synthesized rapidly by the seeds. The increase in starch content in the seeds was followed by a decrease in soluble carbohydrate content, after which the seed ceased to accumulate water. These metabolic changes are discussed in the light of recent biochemical knowledge, and in relation to more detailed biochemical investigations in progress.

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“…Total carbohydrates were determined with the anthrone reagent [8], and glucose content by the Glucostat method (Worthington, New Jersey, U.S.A.). Amino acids analysis was carried out by BioCal amino acid analyzer.…”

Section: Methodsmentioning

confidence: 99%

Germination-inhibitor in slime mould Physarum polycephalum

Chet

1977

FEMS Microbiology Letters

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Anthrone in Estimation of Hexose Sugars with Special Reference to Pentose Interference

Cited by 36 publications

References 5 publications

Reproducible induction of cavity spot in carrots and physiological and microbial changes occurring during cavity formation

Reproducible induction of cavity spot in carrots and physiological and microbial changes occurring during cavity formation

Physiology of Pea Fruits I. the Developing Fruit

Germination-inhibitor in slime mould Physarum polycephalum

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