Anthrone in Estimating Low Concentrations of Sucrose

Morse, Edward E.

doi:10.1021/ac60012a021

Cited by 77 publications

(39 citation statements)

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“…Soluble carbohydrates were extracted by treating replicate (n = 2) subsamples of 200 mg with hot (100°C) 2 M HCl for 2.5 h (Hashimoto et al 1987). Total soluble carbohydrates were determined by the Anthron method (Morse 1949) using glucose as a standard.…”

Section: Methodsmentioning

confidence: 99%

Foraging by the stoplight parrotfish Sparisoma viride II. Intake and assimilation of food, protein and energy

Bruggemann¹,

Begeman²,

Bosma³

et al. 1994

Mar. Ecol. Prog. Ser.

100

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ABSTRACT. Daily food intake by the herbivorous parrotfish Sparisoma v~nde, as well as assimdation efficiencies of algal food, protein and energy, were quantified through a combination of laboratory feeding tnals and field observations. The intake of algal ash-free dry wt (AFDW) per bite increases hnearly with fish wet wt (FWW, g) and algal biomass (mg AFDW cm-2), and is further determined by the skeletal density of the limestone substrate. Low-density substrates yield higher amounts of AFDW per bite than do high-density substrates. The percentage of the total food intake that is derived from endolithic and crustose coralline algae increases with the size of the flsh, and can be 9 0 % depending on the biomass of epilithic algae. The daily pattern of foraging activity is positively correlated with diurnal changes in food quality, while seasonal daylength variations result in 13 % variation in total daily bites taken. Daily number of bites of S. viride in the field decreases wlth fish size, and is further dependent on life phase and foraging depth. In experiments, fish attained an assimilation efficiency of ca 20% from a natural diet of low algal biomass and high-density dead coral substrates that predominates in the shallow reef. Assimilation efficiency was ca 70 % from a diet of high algal biomass and lowdensity substrates that predominates on the deeper reef parts. In spite of lower daily foraging effort, territorial fish, living in deeper parts of the reef, ingest and assimilate higher amounts of AFDW, protein and energy per day than non-territorial fish foraging on the shallow reef. The difference is caused by increased availability of high-yield food and substrate types inside territories compared to the situation on the shallow reef. Daily assimilated energy (kJ d-') is 0.85 X FWW'.~'~ for fish foraging in the shallow reef zone, and 1.22 X FWW0854 for S. viride foraging inside territories on the deeper reef.

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Section: Methodsmentioning

confidence: 99%

Foraging by the stoplight parrotfish Sparisoma viride II. Intake and assimilation of food, protein and energy

Bruggemann¹,

Begeman²,

Bosma³

et al. 1994

Mar. Ecol. Prog. Ser.

100

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show abstract

“…Free sugar content of chickpea seeds was determined calorimetrically by the anthrone method (Morse 1947). Reducing sugar content of chickpea seeds was estimated by DNS (Dinitrosalicylic acid) method (Miller 1972).…”

Section: Degradation Of Nutrients During Germination Of Chickpea Seedmentioning

confidence: 99%

“…Reducing sugar content of chickpea seeds was estimated by DNS (Dinitrosalicylic acid) method (Miller 1972). The starch content of chickpea seeds was determined by the anthrone method (Morse 1947, Jayaraman 1985 and lipid content of chickpea seeds was determined by the method of Bligh and Dyer (1959).…”

Section: Degradation Of Nutrients During Germination Of Chickpea Seedmentioning

confidence: 99%

Enzyme Activities and Degradation of Nutrients in Chickpea (<i>Cicer arietinum</i> L.) Seeds during Germination

Sana

Hasan

et al. 1970

J bio-sci.

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Enzyme activities and degradation of seed storage substances were investigated in BARI-1, BARI-2 and BARI-3 varieties of chickpea (Cicer arietinum L.) seed at different germinating periods. Among the varieties, the highest amylase activity was found in BARI-2 and lowest in BARI-3 during germination at 45 hours in water. The maximum activity of invertase was found in BARI-2 and minimum in BARI-1 at 72 hours of germination. Lipase activity was highest in BARI-2 and lowest in BARI-1. The highest protease activity was found in BARI-2 and lowest in BARI-3. The amount of total protein and water-soluble protein were found to be highest in BARI-1 and BARI-3, respectively. During light germination, carbohydrate depletion starts after initial imbibitions, and was completed in 120-144 hours. The variety BARI-1 was found to contain the highest amount of free sugar while BARI-3 had the lowest amount. BARI-2 was found to contain highest amount of reducing sugar and BARI-1 contained the lowest amount. The highest amount of starch was found in BARI-3 and lowest in BARI-1. The starch content in chickpea seed decreased gradually during germination. Among the varieties, BARI-1 was found to contain the highest amount of lipid while BARI-3 contained the lowest amount. The seed storage substances were found to decrease gradually with the increase of germination time. The results indicate that degradation of reserve seed nutrients accelerate the development of seedling growth during germination.

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“…Samples of lung homogenate supernatants were added to a solution of 0.05% anthrone (Sigma) in sulfuric acid, mixed well, and allowed to equilibrate for 20 min. Ficoll concentrations were determined by measuring absorbance spectrophotometrically at 627 nm and represent the total Ficoll retained per lung (16).…”

mentioning

confidence: 99%