Anthranilic Acid–uraemic Toxin Damaged Red Cell’s Membrane

Tankiewicz, A; Pawlak, Dariusz; Pawlak, Krystyna; Szewc, D.; Myśliwiec, Michał; Buczko, Włodzimierz

doi:10.1007/s11255-005-0395-x

Cited by 12 publications

(11 citation statements)

References 14 publications

(15 reference statements)

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“…Thus, this exploratory study shows that Raman spectroscopy can be used in studying the head and neck squamous cell carcinoma. Our study shows increased levels of tryptophan and decreased levels of keratin as the tissue changes from normal to squamous cell carcinoma, which is consistent with other biochemical investigations …”

Section: Discussionsupporting

confidence: 93%

“…It is interesting to note that a recent study by Tankiewicz et al . has shown an enhancement in the concentration of tryptophan and its derivatives (anthralinic and kynurenic acids) in oral tongue squamous cell carcinoma leading to a proposition that tryptophan may be a factor responsible for the development of cancer . An earlier study by Crowe et al .…”

Section: Resultsmentioning

confidence: 98%

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Diagnosis of head and neck squamous cell carcinoma using Raman spectroscopy: tongue tissues

Devpura

Thakur

Sethi

et al. 2011

J Raman Spectroscopy

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Raman spectroscopy is a powerful optical technique capable of providing the structural information at the molecular level. Thus, the technique can be used to detect biochemical changes associated with carcinogenesis and identify the biomolecules involved in cancer. We studied the Raman spectral characteristics of normal, carcinoma in situ, and invasive squamous cell carcinoma (SCC) tissues of tongue, and identified the spectral features that can discriminate these three tissue types. We found that the intensities of Raman bands assignable to tryptophan increase while those attributable to protein keratin decrease when tissue changes from normal to invasive SCC. The variation observed in the intensity of many discriminating peaks including those of tryptophan and keratin as tissue changes from normal to carcinoma in situ and then to invasive SCC suggests that Raman spectroscopy can be used to monitor progression of the disease. We have also analyzed the data with multivariate statistical methods such as principal component analysis and discriminant function analysis. These chemometric methods clearly separate the whole data into three distinct groups consistent with results of pathology. We were able to detect with 91% success rate the normal and carcinoma in situ tissues and with 89% accuracy the invasive SCC tissues of the tongue. Copyright © 2011 John Wiley & Sons, Ltd.

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 98%

Diagnosis of head and neck squamous cell carcinoma using Raman spectroscopy: tongue tissues

Devpura

Thakur

Sethi

et al. 2011

J Raman Spectroscopy

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“…[39] Tankiewicz et al demonstrated the change of tryptophan metabolism, which is reflected by the increase in the concentrations of tryptophan and its metabolites in patients with oral SCC. [40] The 960 cm −1 peak predominately occurred in tumor squamous cells (Table S1, supporting information). Following an extensive literature search, no reasonable explanation for this peak to appear in the SCC was found.…”

Section: Discussionmentioning

confidence: 97%

Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity

Guze

Short

Zeng

et al. 2010

J Raman Spectroscopy

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Raman spectroscopy has been effectively applied to clinically differentiate normal and cancerous mucosal tissues. Micro-Raman spectroscopy provides a tool to better understand the molecular basis for the Raman clinical signal. The objective of the current study was to utilize micro-Raman spectroscopy to define the molecular/spectral differences between normal and abnormal squamous cell carcinoma (SCC) in oral mucosa (in vitro). Understanding this may help in identifying unique spectra or may be useful for in vivo application of this technology. Micro-Raman (confocal) spectroscopy was used to obtain molecular images of normal and SCC cells of human oral mucosa. Four fresh flashed-frozen tumor and four matched normal tongue specimens were studied. The spectra covered a wavenumber range from 300 to 4000 cm −1 with a spectral resolution of 8 cm −1 and a spatial resolution of 1.0 µm. The cells were located within thin sections of tongue mucosa biopsies. The excitation wavelength of 515 nm was used. We were able to obtain Raman images with rich information about the spectroscopic and structural features within the cytoplasm, cell membrane, and cell nuclei. Significant spectral differences were observed between the Raman images of normal and malignant squamous cells. The heterogeneity of tumor cells within the abnormal tissue was also demonstrated. Spectral differences demonstrated between both tissue types have provided important information regarding the origins of specific signals within the cells of each tissue type. In our search for specific spectral biomarkers, we believe that a cell surface protein, greatly upregulated in SCC cells, was discovered at 1583 cm −1 .

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“…For evaluation of the osmotic resistance of erythrocytes, blood samples were collected into EDTA-K 2 test tubes from the rats left ventricle and were tested according to Hunter [ 44 ] in our modification [ 45 ]. The osmotic resistance test was performed directly after blood withdrawn.…”

Section: Methodsmentioning

confidence: 99%

The Uremic Toxin Indoxyl Sulfate Accelerates Thrombotic Response after Vascular Injury in Animal Models

Karbowska

Kamiński

Marcińczyk

et al. 2017

Toxins

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Chronic kidney disease (CKD) patients are at high risk for thrombotic events. Indoxyl sulfate (IS) is one of the most potent uremic toxins that accumulates during CKD. Even though IS is associated with an increased risk for cardiovascular disease, its impact on thrombotic events still remains not fully understood. The purpose of the study was to evaluate the direct effect of IS on thrombotic process. We examined the impact of acute exposure to IS on thrombus development induced by electric current in Wistar rats, intravital thrombus formation after laser-induced injury in the mice endothelium, coagulation profile, clot formation dynamics, platelet aggregations, and erythrocyte osmotic resistance. IS doses: 10, 30 and 100 mg/kg body weight (b.w.) increased weight of thrombus induced by electric current in dose-dependent manner (p < 0.001). Furthermore, two highest IS doses increased laser-induced thrombus formation observed via confocal system (increase in fluorescence intensity and total thrombus area (p < 0.01)). Only the highest IS dose decreased clotting time (p < 0.01) and increased maximum clot firmness (p < 0.05). IS did not affect blood morphology parameters and erythrocyte osmotic resistance, but augmented collagen-induced aggregation. Obtained data indicate that IS creates prothrombotic state and contributes to more stable thrombus formation. Thus, we concluded that IS may be one of crucial uremic factors promoting thrombotic events in CKD patients.

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Anthranilic Acid–uraemic Toxin Damaged Red Cell’s Membrane

Cited by 12 publications

References 14 publications

Diagnosis of head and neck squamous cell carcinoma using Raman spectroscopy: tongue tissues

Diagnosis of head and neck squamous cell carcinoma using Raman spectroscopy: tongue tissues

Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity

The Uremic Toxin Indoxyl Sulfate Accelerates Thrombotic Response after Vascular Injury in Animal Models

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