Anthracnose of Sansevieria trifasciata caused by Colletotrichum sansevieriae sp. nov.

Nakamura, Masayuki; Ohzono, Masahiro; Iwai, Hisashi; Arai, K.

doi:10.1007/s10327-006-0280-1

Cited by 34 publications

(38 citation statements)

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“…Colletotrichum sansevieriae is an anthracnose pathogen identified as a new species in 2006 [24]. In 1996, the fungus caused water-soaked lesions and leaf blight on Sansevieria trifasciata Prain var.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-Mediated Transformation for Investigating Pathogenicity Genes of the Phytopathogenic Fungus Colletotrichum sansevieriae

et al. 2012

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Agrobacterium tumefaciens-mediated transformation (AtMT) has become a common technique for DNA transformation of yeast and filamentous fungi. In this study, we first established a protocol of AtMT for the phytopathogenic fungus Colletotrichum sansevieriae. Binary T-DNA vector containing the hygromycin B phosphotransferase gene controlled by the Aspergillus nidulans gpdA promoter and the trpC terminator was constructed with pCAMBIA0380 and used with three different strains LBA4404, GV3101, and GV2260 of A. tumefaciens. Transformants were most effectively obtained when GV2260 and C. sansevieriae Sa-1-2 were co-cultivated; there were about 320 transformants per 10(6) spores. When 1,048 transformants were inoculated on Sansevieria trifasciata, three transformants were found to have completely lost their pathogenicity and two transformants displayed reduced pathogenicity. All of the five transformants had a single copy of T-DNA in their genomes. The three pathogenicity-deficient transformants were subjected to thermal asymmetric interlaced polymerase chain reaction and the reaction allowed us to amplify the sequences flanking the left and/or right borders. The flanking sequences of the two transformants, M154 and M875, showed no homology to any sequences in databases, but the sequences of M678 contained motifs of alpha-1,3-glucan synthase, suggesting that the gene might contribute to the pathogenicity of C. sansevieriae. This study describes a useful method for investigating pathogenicity genes in C. sansevieriae.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-Mediated Transformation for Investigating Pathogenicity Genes of the Phytopathogenic Fungus Colletotrichum sansevieriae

et al. 2012

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“…La antracnosis de la Sansevieria, fue descubierta en el año 2006 (Nakamura et al, 2006) y es causada por una nueva especie del género Colletotrichum (C. sansevieriae sp. nov.).…”

Section: Introductionunclassified

“…sansevieriae se considera un patógeno restringido a sansevierias, es decir, con un alto grado de especificidad por el hospedante (Nakamura et al, 2006;Aldaoud et al, 2011;Palmateer et al, 2012;Pérez-León et al, 2013). Algunas especies de Sansevieria han mostrado tolerancia al patógeno, incluidas algunas variedades de la especie S. trifasciata (Pérez-León et al, 2013).…”

Section: Introductionunclassified

“…Algunas especies de Sansevieria han mostrado tolerancia al patógeno, incluidas algunas variedades de la especie S. trifasciata (Pérez-León et al, 2013). Sin embargo, las variedades con mayor demanda en el mercado son susceptibles (Nakamura et al, 2006;Aldaoud et al, 2011;Pérez et al, 2013), por lo que el control químico de la enfermedad es por el momento la opción más viable para su manejo.…”

Section: Introductionunclassified

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Combate químico de la antracnosis de Sansevieria trifasciata var. Hahnii en un sistema de hojas separadas.

et al. 2015

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RESUMENCombate químico de la antracnosis de Sansevieria trifasciata var. Hahnii en un sistema de hojas separadas. El objetivo de este trabajo fue evaluar fungicidas para el combate de antracnosis en Sansevieria trifasciata var. Hahnii. Se evaluaron doce fungicidas (solos o en mezcla) sobre la infección y severidad de C. sansevieriae en un sistema de hojas separadas. Los fungicidas utilizados fueron azoxistrobina, boscalid + piraclostrobina, carbendazina + mancozeb, difenoconazol, epoxiconazol + carbendazina, folpet, imazalil, metil tiofanato + mancozeb, miclobutanil y prochloraz. El estudio se llevó a cabo en el Laboratorio de Biotecnología de Plantas del Centro de Investigaciones Agronómicas, Universidad de Costa Rica, durante el primer semestre del año 2012. La aplicación de los tratamientos se realizó por aspersión el día de la inoculación o tres días después de esta (0 y 3 ddi). A los siete, once y quince días después de la inoculación se evaluó el número y diámetro de las lesiones. El fungicida, el momento de aplicación, la interacción fungicida x momento de aplicación y la interacción fungicida x día de evaluación afectaron significativamente (p<0,0001) las variables evaluadas. Azoxistrobina, boscalid + piraclostrobina, carbendazina + mancozeb, epoxiconazol + carbendazina y metil tiofanato + mancozeb proveyeron un 100% de protección de las hojas de Sansevieria durante todo el período de evaluación (quince días), cuando se aplicaron el mismo día de la inocualción (0 ddi). A los 3 ddi, solo epoxiconazol + carbendazina inhibió en un 100% el establecimiento del patógeno (incidencia cero), en las tres evaluaciones. Con los tratamientos azoxistrobina y carbendazina + mancozeb también se obtuvo un 0% de incidencia a los siete y once días de evaluación para el primero y a los siete días para el segundo.Palabras clave: Lengua de suegra, Colletotrichum sansevieriae, fungicidas para C. sansevieriae. ABSTRACTChemical control of anthrachnose of Sansevieria trisfasciata var. Hahnii on a detached-leaf system. The objective of this work was to assess fungicides to combat Sansevieria trifasciata var. Hahnii anthracnose. Twelve fungicides (alone or in mixture) were evaluated on the infection and severity of C. sansevieriae on a detached-leaf system. Fungicides used were azoxystrobin, boscalid + pyraclostrobin, carbendazim + mancozeb, difenoconazole, epoxiconazole + carbendazim, folpet, imazalil, thiophanatemethyl + mancozeb, myclobutanil, and prochloraz. The study was carried out at the Plant Biotechnology Laboratory of the Agricultural Research Center of the University of Costa Rica, during the first semester of 2012. Each chemical treatment was applied by aspersion on the day of inoculation (0 dai) or three days after it (3 dai). The number and diameter of the lesions were evaluated after seven, eleven and fifteen dai. Fungicide, application moment, fungicide x application moment interaction and fungicide x evaluation moment interaction significantly affected (p<0,0001) both evaluated variables. Azoxystrobin, boscalid + p...

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“…Besides its attractive dark and light green variegation, S. trifasciata is tolerant to a wide variety of habitats. Sansevieria trifasciata were reported to be infected by Colletotrichum sansevieriae (Nakamura et al 2006) and Chaetomella sp. (Li et al 2013) associated with anthracnose and leaf spot, respectively.…”

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Characterisation of Neoscytalidium dimidiatum causing leaf blight on Sansevieria trifasciata in Malaysia

Kee

Suhaimi

Zakaria

et al. 2017

Australasian Plant Dis. Notes

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Sansevieria trifasciata showing symptoms of leaf blight were observed in several states of Malaysia. Based on morphology and DNA sequences of the Internal Transcribed Spacer (ITS) region, nine isolates of Neoscytalidium dimidiatum were identified. Phylogenetic analysis grouped the isolates of N. dimidiatum from S. trifasciata with the epitype of Neoscytalidium dimidiatum (CBS499.66) from mango. Pathogenicity test showed that all isolates of N. dimidiatum caused leaf blight on S. trifasciata and fulfilled Koch's postulates.Keywords Neoscytalidium dimidiatum . Leaf blight . Sansevieria trifasciata . ITS Sansevieria trifasciata, commonly known as mother-in-law's tongue, is a popular ornamental plant that can be found throughout Malaysia. Besides its attractive dark and light green variegation, S. trifasciata is tolerant to a wide variety of habitats. Sansevieria trifasciata were reported to be infected by Colletotrichum sansevieriae (Nakamura et al. 2006) and Chaetomella sp. (Li et al. 2013) associated with anthracnose and leaf spot, respectively. In 2015, another disease symptom, leaf blight was observed on S. trifasciata in several states of Malaysia. Scytalidium-like fungal isolates were recovered during preliminary fungal isolation from the samples.During September 2015 and March 2017, diseased S. trifasciata with symptoms of leaf blight were collected from Penang and three other Malaysian states, namely Perak, Negeri Sembilan and Sarawak. Symptomatic samples were cut into approximately 1.5 cm 2 fragments and surface sterilised in 70% ethanol (C 2 H 5 OH) solution and 1% sodium hypochlorite (NaOCl) solution for 3 min each. Then, the samples were rinsed thrice in sterile distilled water for 1 min each before being plated on potato dextrose agar (PDA) and incubated at 25 ± 3°C for 3 to 5 days. Pure cultures were obtained by transferring a single conidium from the colony grown from surface sterilised samples onto fresh PDA and incubated as described previously. A mycelial plug 6 mm in diameter taken from a 7-days-old culture was transferred onto a PDA plate. The growth rate was recorded by measuring the colony diameter daily until the mycelia fully covered the plate. The isolates were cultured on 2% water agar (WA) overlaid with sterilised horsetail twigs (Casuarina equisetifolia) and carnation leaves, then were incubated as described previously to induce pycnidia formation. Measurements of 50 randomly selected conidia from pycnidia and arthrospores were taken.

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Anthracnose of Sansevieria trifasciata caused by Colletotrichum sansevieriae sp. nov.

Cited by 34 publications

References 3 publications

Agrobacterium tumefaciens-Mediated Transformation for Investigating Pathogenicity Genes of the Phytopathogenic Fungus Colletotrichum sansevieriae

Agrobacterium tumefaciens-Mediated Transformation for Investigating Pathogenicity Genes of the Phytopathogenic Fungus Colletotrichum sansevieriae

Combate químico de la antracnosis de Sansevieria trifasciata var. Hahnii en un sistema de hojas separadas.

Characterisation of Neoscytalidium dimidiatum causing leaf blight on Sansevieria trifasciata in Malaysia

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