Anthosphere Microbiome and Their Associated Interactions at the Aromatic Interface

Arunkumar, Nagarathinam; Rakesh, Suchitra; Rajaram, Kaushik; Kumar, Narayanasamy Ravi; Durairajan, Siva Sundara Kumar

doi:10.1007/978-3-030-19831-2_14

Cited by 8 publications

(5 citation statements)

References 71 publications

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“…These microorganisms can be endophytic or epiphytic and can be transferred by wind, rain, seeds, and pollinators [ 69 ]. The representatives of anthospheres were characterized by high diversity, but some species of Pseudomonas and Acinetobacter (Proteobacteria), Metschnikowia (Ascomycota), and Cryptococcus (Basidiomycota) were constantly recorded [ 11 , 70 ], which was also confirmed by the results obtained in this study. The abundance of fungi (especially diverse population of yeast) surpassing that of bacteria in the stigma may be linked to a fertile environment with ample nutrients and optimal moisture levels conducive to fungal growth.…”

Section: Discussionsupporting

confidence: 86%

Stigmas of holoparasitic Phelipanche arenaria (Orobanchaceae) – a suitable ephemeric flower habitat for development unique microbiome

Ruraż,

Przemieniecki,

Błaszak

et al. 2023

BMC Plant Biol

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Background Microbial communities have occasionally been observed in part of the ephemeric reproductive structure of floral stigmas, but their prevalence, phylogenetic diversity and ecological roles are understudied. This report describes the first study of bacterial and fungal communities in immature and mature stigma tissue of the endangered holoparasitic plant Phelipanche arenaria. Culture-dependent methods coupled with next-generation sequencing indicated that a small surface of the flower stigma was an unexpectedly rich and diverse microhabitat for colonization of microbial. We also compared the enzymatic activity of the bacterial communities between immature and mature stigmas samples. Results Using high-throughput sequencing methods, we identified and classified 39 to over 51 OTUs per sample for bacterial OTUs represented by Pantoea agglomerans and P. ananatis, comprising 50.6%, followed by Pseudomonas, Luteibacter spp., Sphingomonas spp. with 17% of total frequency. The bacterial profile of immature stigmas of P. arenaria contained unique microorganisms (21 of the most numerous OTUs) that were not confirmed in mature stigmas. However, the enzymatic activity of bacteria in mature stigmas of P. arenaria showed more activity than observed in immature stigmas. In the fungal profile, we recorded even 80 OTUs in mature stigmas, consisting of Capnodiales 45.03% of the total abundance with 28.27% of frequency was created by Alternaria eichhorniae (10.55%), Mycosphaerella tassiana (9.69%), and Aureobasidium pullulans (8.03%). Additionally, numerous putative plant growth-promoting bacteria, fungal pathogens and pathogen-antagonistic yeasts were also detected. Conclusions Our study uncovered that P. arenaria stigmas host diverse bacterial and fungal communities. These microorganisms are well known and have been described as beneficial for biotechnological and environmental applications (e.g., production of different enzymes and antimicrobial compounds). This research provided valuable insight into the parasitic plant–microbe interactions.

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Section: Discussionsupporting

confidence: 86%

Stigmas of holoparasitic Phelipanche arenaria (Orobanchaceae) – a suitable ephemeric flower habitat for development unique microbiome

Ruraż,

Przemieniecki,

Błaszak

et al. 2023

BMC Plant Biol

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“…The underlying mechanisms and recruited microorganisms of this strategy, however, are unclear and probably distinct across plant compartments. Strikingly, a board spectrum of bacteria and fungi (e.g., yeasts) inhabit the anthosphere (i.e., flowers and surrounding zones), especially nectar, pollen (Vannette et al, 2013 ; Schaeffer et al, 2017 ) and flower surface (Ushio et al, 2015 ; Arunkumar et al, 2019 ), which significantly influence flower‐pollinator interactions, plant reproduction and yield. Due to the diverse microbes transmitted from various sources, flowers are potentially dynamic hubs of microbes and pollinators.…”

Section: Potential Functions and Interactions Of Protists In The Plan...mentioning

confidence: 99%

Plant associated protists—Untapped promising candidates for agrifood tools

Nguyen

Dumack

Trivedi

et al. 2022

Environmental Microbiology

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The importance of host-associated microorganisms and their biotic interactions for plant health and performance has been increasingly acknowledged. Protists, main predators and regulators of bacteria and fungi, are abundant and ubiquitous eukaryotes in terrestrial ecosystems. Protists are considered to benefit plant health and performance, but the community structure and functions of plant-associated protists remain surprisingly underexplored. Harnessing plant-associated protists and other microbes can potentially enhance plant health and productivity and sustain healthy food and agriculture systems. In this review, we summarize the knowledge of multifunctionality of protists and their interactions with other microbes in plant hosts, and propose a future framework to study plant-associated protists and utilize protists as agrifood tools for benefiting agricultural production.

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“…This is in contrast to epiphytic microbial colonization of the stigmas, which cannot be protected from dehydration and pathogen attack in the form of thick cuticle or wax because they must capture and hydrate pollen. These microhabitats have their own and specific compositions of associated microorganisms, and some florivores and insect-like pollinators may dynamically change this niche 23 . The parts of flowers are a unique environment for microbial communities because they contain multiple niches differing in morphology, chemical composition and longevity.…”

Section: Introductionmentioning

confidence: 99%

Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

Ruraż

Przemieniecki

Piwowarczyk

2023

Sci Rep

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Little is known about the microbiomes of flower parts, and even less information is available regarding these microorganisms’ colonization of specific niches in parasitic plants. We investigate the temporal interspecies dynamics of the parasitic plants microbiome of flower stigmas in two stages of development: immature stigmas in flower buds and mature stigmas in opened flowers. We compared two related holoparasitic Orobanche species from localities approximately 90 km apart and characterize their bacterial and fungal communities using 16S rRNA gene and ITS sequences, respectively. We identified from 127 to over 228 OTUs per sample for fungi, sequences belonging to genera: Aureobasidium, Cladosporium, Malassezia, Mycosphaerella, and Pleosporales, constituting approximately 53% of the community in total. In the bacterial profile, we recorded 40 to over 68 OTUs per sample consisting of Enterobacteriaceae, and genera Cellulosimicrobium, Pantoea, and Pseudomonas spp., with an approximately 75% frequency. In microbial communities, higher numbers of OTUs colonizing mature stigmas were recorded than in immature. This implies that the dynamics and concurrence of microbial communities were different between O. alsatica and O. bartlingii and underwent significant changes during flower development. To the best of our knowledge, is the first study of the interspecies and temporal dynamics of the bacterial and fungal microbiomes of pistil stigmas in flowers.

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Anthosphere Microbiome and Their Associated Interactions at the Aromatic Interface

Cited by 8 publications

References 71 publications

Stigmas of holoparasitic Phelipanche arenaria (Orobanchaceae) – a suitable ephemeric flower habitat for development unique microbiome

Stigmas of holoparasitic Phelipanche arenaria (Orobanchaceae) – a suitable ephemeric flower habitat for development unique microbiome

Plant associated protists—Untapped promising candidates for agrifood tools

Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

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