Anthocyanins and Carotenoids Characterization in Flowers and Leaves of Cyclamen Genotypes Linked with Bioactivities Using Multivariate Analysis Techniques

Cornea-Cipcigan, Mihaiela; Bunea, Andrea; Bouari, Cosmina; Pamfil, Doru; Páll, Emöke; Urcan, Adriana Cristina; Mărgăoan, Rodica

doi:10.3390/antiox11061126

Cited by 16 publications

(14 citation statements)

References 102 publications

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“…Third, anthocyanins can also interact with other colorless molecules, such as copigments [ 35 ] or other anthocyanins [ 36 ], to enhance the color. Such a theory is also supported by the later intermolecular stacking theory [ 37 ]. For instance, polyacylated anthocyanins can form stable hydrophobic interactions that give blue color even without copigment or metal ions in a wide pH range.…”

Section: Discussionmentioning

confidence: 58%

Anthocyanins Profiling Analysis and RNA-Seq Revealed the Dominating Pigments and Coloring Mechanism in Cyclamen Flowers

Xia

Wang

et al. 2022

Biology

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Pigments in cyclamen (Cyclamen purpurascens) endows flowers with great ornamental and medicinal values. However, little is known about the biosynthetic pathways of pigments, especially anthocyanins, in cyclamen flowers. Herein, anthocyanins profiling and RNA-Seq were used to decipher the molecular events using cyclamen genotypes of red (HXK) or white (BXK) flowers. We found that red cyclamen petals are rich in cyanidin-3-O-rutinoside, cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, malvidin-3-O-glucoside, peonidin-3-O-rutinoside, quercetin-3-O-glucoside, and ruti. In addition, our transcriptomics data revealed 3589 up-regulated genes and 2788 down-regulated genes comparing the BXK to HXK. Our rich dataset also identified eight putative key genes for anthocyanin synthesis, including four chalcone synthase (CHS, g13809_i0, g12097_i0, g18851_i0, g36714_i0), one chalcone isomerase (CHI, g26337_i0), two flavonoid 3-hydroxylase (F3′H, g14710_i0 and g15005_i0), and one anthocyanidin synthase (ANS, g18981_i0). Importantly, we found a 2.5 order of magnitude higher expression of anthocyanin 3-O-glucosyltransferase (g8206_i0), which encodes a key gene in glycosylation of anthocyanins, in HXK compared to BXK. Taken together, our multiomics approach demonstrated massive changes in gene regulatory networks and anthocyanin metabolism in controlling cyclamen flower color.

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Section: Discussionmentioning

confidence: 58%

Anthocyanins Profiling Analysis and RNA-Seq Revealed the Dominating Pigments and Coloring Mechanism in Cyclamen Flowers

Xia

Wang

et al. 2022

Biology

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“…A phenotypic trait that often reflects the physiological status of the plant is the color of the flowers and leaves ( Noman et al., 2017 ; Cui et al., 2019 ). The concentration of secondary metabolites with considerable chromatic elements, that may have decorative, medicinal, and economic relevance, is indicated by the color of the flowers and leaves ( Cornea-Cipcigan et al., 2022a ). Color variation also gives data on the genetic inter- and intraspecific diversity of populations and/or species, recognizing the progress of evolutionary principles from a phylogenetic standpoint ( Tanaka et al., 2008 ; Klančnik et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to their ornamental importance, Cyclamen sp. also possesses strong antioxidant properties due to the phenolic composition, and anti-cancer activities reported in HeLa, non-small cell lung cancer H1299 cells, human colorectal cancer cells (HCT 116 and HT-29), human breast adenocarcinoma (MDA-MB-231) and human fibroblasts BJ cells ( Mihci-Gaidi et al., 2010a ; Mihci-Gaidi et al., 2010b ; Yildiz et al., 2013 ; Cornea-Cipcigan et al., 2022a ). Due to a shortage of plant material, their use for the production of pharmaceuticals has become a severe problem, potentially leading to the loss of plant populations and variety, natural habitat deterioration, and/or species extinction.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Cyclamen genotypes using morphological descriptors and DNA molecular markers in a multivariate analysis

Cornea-Cipcigan

Pamfil²,

Sisea³

et al. 2023

Front. Plant Sci.

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Morphological and molecular characterization of germplasm is essential for the improvement of cultivated plants efforts. This study investigated the genetic diversity of 32 Cyclamen genotypes comprising 16 C. persicum varieties and 16 Cyclamen species using multivariate analysis for 36 morphological traits (19 quantitative and 17 qualitative) and molecular characterization (SRAP and SCoT markers). The color CIELab parameters were collected via PCE-CSM7 that separately measured the leaves dark and silvery patterns and the flower’s slip (region of the petal top), eye (the region of the petal base) and sepal. Genetic diversity was also evaluated using Shannon Wiener (H′) and Simpson’s (λ) Indices, and Pilou evenness (J) using the library vegan from R software. According to the principal component analysis, the variables that contributed the most were leaf pattern color, leaf abaxial surface color, pedicel coiling, leaf and stem number. The color indicators of Cyclamen leaves showed decreased L* values in darker colored genotypes, whereas increased a* values were noticed in flower eye and lower in slip. Molecular characterization was based on 26 SRAP and 12 SCoT markers that produced clearly repeatable DNA bands and exhibited significant levels of polymorphism. Based on the morphological traits and molecular markers data, the UPGMA method for hierarchical clustering technique was used to generate the dendrograms, and their entanglement was obtained using the Tanglegram algorithm from the dendextend package with the R software. Entanglement analysis (0.30) between dendrograms obtained from the morphological and genetic analysis using SRAP markers showed a high association. Comparison between color measurements of flowers (entanglement=0.45) and leaves (entanglement=0.47) with SCoT analysis revealed differences at species level, discriminating between similar genotypes. Combined phenotypic and molecular analysis improved the comprehensive estimation of real diversity in the investigated Cyclamen genotypes. The findings of the present study are useful for quantifying diversity and genetic variability in Cyclamen breeding and genetic investigations.

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“…Spectrophotometric readings at 450 nm were performed with a BioTek Synergy 2 microplate reader (Winooski, VT, USA). Data are shown as optical density or average proliferation percentage rate compared with the control of untreated cells [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

Chemical Profile and Biological Effects of an Herbal Mixture for the Development of an Oil-in-Water Cream

Safta

Ielciu

Șuștic

et al. 2023

Plants

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Three individual hydroalcoholic extracts derived from Hamamelis virginiana leaves, Krameria lappacea root, Salix alba bark, and the resulting herbal mixture (HM) were assessed for the phytochemical profile as well as for antibacterial and cytotoxic potential. The chemical composition of the individual extracts and of their mixture was analyzed by chromatographical (LC-MS) and spectrophotometrical methods. The antimicrobial properties were evaluated by using the agar-well diffusion and the broth microdilution assays, whereas the potential cytotoxicity was investigated on human keratinocyte cell line by MTT method and apoptosis test. The HM composition revealed important amounts of valuable polyphenolic compounds provided from the individual extracts, having synergistic biological effects. All tested extracts displayed in vitro antimicrobial properties, with a significantly higher efficacy noticed for the HM when tested against Staphylococcus aureus. Moreover, none of the tested extracts was responsible for in vitro cytotoxicity against the human keratinocytes in the selected concentration range. Furthermore, the HM was included in an oil-in-water cream for the nonpharmacological treatment of seborrheic dermatitis, developed and optimized by using a QbD approach. A D-optimal experimental plan with four factors that varied on two levels was used to investigate the effect of the quantitative variation of the formulation factors (emulsifier, co-emulsifier, thickening agent, oily phase ratio) on the characteristics of the cream in terms of firmness, consistency, adhesiveness, stringiness, spreadability, and viscosity. Based on the experimental results, an optimal formulation containing 2.5% emulsifier and 20% oily phase was prepared and analyzed. The obtained results showed appropriate quality characteristics of this novel cream, which may be used in the future to manage the associated symptoms of seborrheic dermatitis.

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Anthocyanins and Carotenoids Characterization in Flowers and Leaves of Cyclamen Genotypes Linked with Bioactivities Using Multivariate Analysis Techniques

Cited by 16 publications

References 102 publications

Anthocyanins Profiling Analysis and RNA-Seq Revealed the Dominating Pigments and Coloring Mechanism in Cyclamen Flowers

Anthocyanins Profiling Analysis and RNA-Seq Revealed the Dominating Pigments and Coloring Mechanism in Cyclamen Flowers

Characterization of Cyclamen genotypes using morphological descriptors and DNA molecular markers in a multivariate analysis

Chemical Profile and Biological Effects of an Herbal Mixture for the Development of an Oil-in-Water Cream

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