Anthocyanin Metabolite and Transcriptome Sequencing Analysis Reveals White Flowers in Rhododendron dauricum var. albiflorum
Huaizhi Mu,
Xuhong Jin,
Yue Zhang
et al.
Abstract:Rhododendron dauricum is a cold-hardy shrub integrating ornamental, medicinal, and aromatic functions. Flower color is an important feature related to ornamental value for breeders and consumers. Nevertheless, the coloration mechanism of flower color in R. dauricum is still unclear. R. dauricum var. albiflorum is a white flower variety of R. dauricum. In this study, an integrative analysis of the metabolome and transcriptome was conducted between R. dauricum var. albiflorum and R. dauricum. A total of nine ant… Show more
“…In a study of the wild type of primrose (yellow petals) and its variants (yellow transformed to red), it was found that the orange and red primrose types contain anthocyanins such as cyanidin-3-O-glucoside [23]. Most of the current research on rhododendrons focuses on white rhododendrons and red rhododendrons, and many studies on white rhododendrons have shown that white rhododendrons have lower anthocyanin contents [22,24,25]. Additionally, the enzyme genes CHS, F3 ′ 5 ′ H, FLS, I2 ′ H, HID, DFR, and LAR may have a role in the alteration of petal colour in R. liliiflorum [21,[26][27][28].…”
Section: Introductionmentioning
confidence: 99%
“…Metabolomics is widely used in research on plant colour formation [25,29,30]. Metabolic group studies have studied four noncolours of wheat, explaining that the reason for the colour difference of wheat is potentially the accumulation of flavonoid compounds [31].…”
Among ornamental plants, the colour of the petals is an important feature. However, the reason for the colour differences of Rhododendron liliiflorum remains unclear. To reveal the differences in the colour of R. liliiflorum, high-efficiency liquid chromatographic collar (UPLC–MS/MS) technology was used to study the yellow and white parts of R. liliiflorum. A total of 1187 metabolites were identified in R. liliiflorum petals, including 339 flavonoid metabolites. Seventy-eight types of flavonoids in these metabolites were found in the yellow and white parts of R. liliiflorum petals, along with 11 other significantly enriched substances. Combining gene expression-related data with differential metabolite data demonstrated effects of enrichment in the flavanonols (fustin), flavonols (epiafzelechin and afzelechin), and flavanones (pinocembrin) of flavonoid biosynthesis; glyccitin, 6″-O-malonylgenistin, and 6-hydroxydaidzein of isoflavonoid biosynthesis; and anthocyanin biosynthesis of malvidin-3-O-galactoside (primulin), delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside (kuromanin), and cyanidin-3-O-rutinoside (keracyanin), which are potentially the contributing factors responsible for the differences in petal colour in R. liliiflorum. This study establishes a connection between the differential metabolites underlying the color differences in the petals of R. liliiflorum and the gene expression in R. liliiflorum. This will provide a foundation for subsequent research on the regulation of flower color in R. liliiflorum and have profound implications for horticultural applications of R. liliiflorum.
“…In a study of the wild type of primrose (yellow petals) and its variants (yellow transformed to red), it was found that the orange and red primrose types contain anthocyanins such as cyanidin-3-O-glucoside [23]. Most of the current research on rhododendrons focuses on white rhododendrons and red rhododendrons, and many studies on white rhododendrons have shown that white rhododendrons have lower anthocyanin contents [22,24,25]. Additionally, the enzyme genes CHS, F3 ′ 5 ′ H, FLS, I2 ′ H, HID, DFR, and LAR may have a role in the alteration of petal colour in R. liliiflorum [21,[26][27][28].…”
Section: Introductionmentioning
confidence: 99%
“…Metabolomics is widely used in research on plant colour formation [25,29,30]. Metabolic group studies have studied four noncolours of wheat, explaining that the reason for the colour difference of wheat is potentially the accumulation of flavonoid compounds [31].…”
Among ornamental plants, the colour of the petals is an important feature. However, the reason for the colour differences of Rhododendron liliiflorum remains unclear. To reveal the differences in the colour of R. liliiflorum, high-efficiency liquid chromatographic collar (UPLC–MS/MS) technology was used to study the yellow and white parts of R. liliiflorum. A total of 1187 metabolites were identified in R. liliiflorum petals, including 339 flavonoid metabolites. Seventy-eight types of flavonoids in these metabolites were found in the yellow and white parts of R. liliiflorum petals, along with 11 other significantly enriched substances. Combining gene expression-related data with differential metabolite data demonstrated effects of enrichment in the flavanonols (fustin), flavonols (epiafzelechin and afzelechin), and flavanones (pinocembrin) of flavonoid biosynthesis; glyccitin, 6″-O-malonylgenistin, and 6-hydroxydaidzein of isoflavonoid biosynthesis; and anthocyanin biosynthesis of malvidin-3-O-galactoside (primulin), delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside (kuromanin), and cyanidin-3-O-rutinoside (keracyanin), which are potentially the contributing factors responsible for the differences in petal colour in R. liliiflorum. This study establishes a connection between the differential metabolites underlying the color differences in the petals of R. liliiflorum and the gene expression in R. liliiflorum. This will provide a foundation for subsequent research on the regulation of flower color in R. liliiflorum and have profound implications for horticultural applications of R. liliiflorum.
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