Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin Alleviates Ultraviolet-Induced Apoptosis of Human Skin Fibroblasts by Regulating the Death Receptor Pathway

Abstract

The study aimed to investigate the potential protective role of anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin against ultraviolet B (UVB)-induced apoptosis of human skin fibroblasts (HSFs). Methods: HSFs cultured in vitro were randomly divided into a control group, UVB group, and anthocyanin groups (0.1, 0.5, and 1.0 mg/mL). HSFs in the UVB and anthocyanin groups were exposed to 30 mJ/cm 2 UVB to establish a photoaging model. Then, apoptosis rate, tumor necrosis factor-α (TNF-α), cysteinyl aspartate specific proteinase-3 (caspase-3), cysteinyl aspartate specific proteinase-7 (caspase-7), and survivin expression were evaluated. Results: UVB irradiation can increase the apoptosis rate of HSFs and expression of TNF-α, caspase-7, and survivin. Anthocyanin pretreatment (0.1, 0.5, and 1.0 mg/mL) decreased UVB-induced apoptosis rate and TNF-α and caspase-7 expression and increased survivin expression. Compared with the control group, the apoptosis rate and expression of TNF-α, caspase-7, and survivin of anthocyanin groups in UVB-irradiated HSFs were high. Among the three doses of anthocyanin (0.1, 0.5, and 1.0 mg/mL) groups, the apoptosis rate and TNF-α expression of anthocyanin at 1.0 mg/mL were the lowest. There was no significant change in caspase-3 expression in each group. Conclusion: Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin could alleviate UVB-induced apoptosis by regulating the death receptor pathway.

METTL14 affects UVB‐induced human dermal fibroblasts photoaging via miR‐100‐3p biogenesis in an m6A‐dependent manner

“…Previous studies have proposed multiple schemes for constructing photoaging cellular models. In this investigation, human dermal fibroblasts (HDFs) were induced using a 30 mJ/cm 2 dose of UVB irradiation to construct a photoaging cellular model that mimics real‐life conditions and is experimentally convenient (Kang et al., 2020 ; Kim et al., 2019 ; Oh et al., 2018 ; Wang et al., 2022 ; Zhang et al., 2023 ).…”

Research Progress on Active Ingredients and Product Development of Lycium ruthenicum Murray

“…Additionally, LRM anthocyanins reduce the cell apoptosis rate through the death receptor pathway, decrease the expression of TNF-α and caspase-7, and increase the expression of survival proteins, thereby exerting a protective effect on human skin fibroblasts (HSFs) exposed to UVB [48,49]. These properties highlight the potential of LRM-derived compounds in promoting eye and skin health.…”