Anthocyanin Extraction Method and Sample Preparation Affect Anthocyanin Yield of Strawberries

Abstract

Anthocyanins are a group of pigments with antioxidant activities that are present naturally in plants. The role of the pigment in human health and its quantitative analysis has attracted a lot of attention globally. A well-known and accurate method of anthocyanin quantification is based on spectrophotometric methods. However, these methods are subject to interference from impurities and need to be optimized for different plant matrixes and extraction conditions. Two experiments were designed to study (1) the effect of plant preparation methods (eg, fresh, frozen, and freeze-dried puree) on anthocyanin yield and (2) the effect of five anthocyanin extraction methods on anthocyanin yield of freeze-dried strawberry puree. Sample preparation methods did not have any effect on anthocyanin yield. Freeze-dried samples were used for their stability (ease of use and flexibility) to compare extraction methods. The anthocyanin yield was affected by the extraction method. Two methods containing chloroform gave the highest anthocyanin yield. One method with methanol:water:HCl gave intermediate results, and the pH differential and the other method with methanol:water:HCl (80:20:1) gave the lowest anthocyanin yields. Processing time (incubation time) was lowest in the pH differential method; however, the haze produced in this method may interfere with the spectrophotometric assessment of anthocyanins.

Aqueous Extract of Onion Peels as A Biowaste-Based Sensitizer for Photovoltaic Cells

“…It was reported elsewhere that anthocyanins are highly soluble in water. Their polyphenolic compounds increase the hydrophobic characteristic, which makes them soluble in organic solvents (38). Furthermore, compared to recently reported natural sensitizers using similar samples and solvents, our power conversion efficiencies of the produced devices are slightly inferior (1,28).…”

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

“…According to annotations, 69% of PsbHLHs were participating in the regulation of biological processes. Anthocyanins are naturally occurring plant pigments with antioxidant properties ( Taghavi et al., 2022 ). Previous research has demonstrated that TT8 regulates anthocyanin biosynthesis ( Ahn et al., 2022 ), and it has been speculated that the homologous gene PsbHLH103 participates in anthocyanin synthesis in P. sibirica .…”

Color and Nutritional Analysis of Ten Different Purple Sweet Potato Varieties Cultivated in China via Principal Component Analysis and Cluster Analysis

“…The anthocyanins have several different structures owing to their reversible transformation with the change in pH of the buffer solution, while the other interferers have no such change. The anthocyanins were present in the form of red closing cations at pH 1.0, while they were colorless semi-acetals or colorless chalcones at pH 4.5 in the buffer solution [ 20 ]. Cypermethrin-3-glucoside was usually used as the reference to quantify the anthocyanins, while most of the anthocyanins in the tested PSPs were highly glycosylated and acylated paeoniflorin anthocyanins, which played an important role in the stability of these phenolics.…”