Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Dong, Guokai; Xu, Nana; Wang, Meng; Zhao, Yunyun; Jiang, Fei; Bu, Huimin; Liu, Jinjuan; Yuan, Bo; Li, Rongpeng

doi:10.3390/ijms222111422

Cited by 24 publications

(13 citation statements)

References 63 publications

(84 reference statements)

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“…In this study, dietary BA supplementation decreased the expression of the above nine immune-related DEGs and the corresponding signaling pathways. Anthocyanin regulated the innate immune system and reduced bacterial systemic dissemination in mice challenged with Klebsiella pneumoniae (Dong et al, 2021 ) and was used as a nutraceutical strategy to modulate stress and immune response in vertebrates (Khan et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

Zhang,

Wang,

et al. 2023

Front. Microbiol.

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IntroductionThe present study investigated the effects of bilberry anthocyanin (BA) on immune function when alleviating Salmonella Typhimurium (S. Typhimurium) infection in chickens.MethodsA total of 180 newly hatched yellow-feathered male chicks were assigned to three groups (CON, SI, and SI + BA). Birds in CON and SI were fed a basal diet, and those in SI + BA were supplemented with 100 mg/kg BA for 18 days. Birds in SI and SI + BA received 0.5 ml suspension of S. Typhimurium (2 × 109 CFU/ml) by oral gavage at 14 and 16 days of age, and those in CON received equal volumes of sterile PBS.ResultsAt day 18, (1) dietary BA alleviated weight loss of chickens caused by S. Typhimurium infection (P < 0.01). (2) Supplementation with BA reduced the relative weight of the bursa of Fabricius (P < 0.01) and jejunal villus height (P < 0.05) and increased the number of goblet cells (P < 0.01) and the expression of MUC2 (P < 0.05) in jejunal mucosa, compared with birds in SI. (3) Supplementation with BA decreased (P < 0.05) the concentration of immunoglobulins and cytokines in plasma (IgA, IL-1β, IL-8, and IFN-β) and jejunal mucosa (IgG, IgM, sIgA, IL-1β, IL-6, IL-8, TNF-α, IFN-β, and IFN-γ) of S. Typhimurium-infected chickens. (4) BA regulated a variety of biological processes, especially the defense response to bacteria and humoral immune response, and suppressed cytokine–cytokine receptor interaction and intestinal immune network for IgA production pathways by downregulating 6 immune-related proteins.ConclusionIn summary, the impaired growth performance and disruption of jejunal morphology caused by S. Typhimurium were alleviated by dietary BA by affecting the expression of immune-related genes and proteins, and signaling pathways are related to immune response associated with immune cytokine receptors and production in jejunum.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

Zhang,

Wang,

et al. 2023

Front. Microbiol.

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“…Dong et al. reported that purple sweet potato anthocyanins (PSPAs) inhibited NLRP3 inflammasome activation by promoting parkin-dependent mitophagy and reducing the release of mtROS and mtDNA; this resulted in reduced lung inflammation and mortality of Klebsiella pneumoniae -infected mice ( 114 ). Suhuang exhibited a positive effect on mitochondrial homeostasis in ALI mice by reducing mtROS overproduction and mtDNA release, downregulating MMP9 expression, and inhibiting NF-κB and NLRP3 inflammasome activation ( 115 ).…”

Section: Organ-level Evaluation Of Trauma-related Sirs Induced By Mtd...mentioning

confidence: 99%

The role of mtDAMPs in the trauma-induced systemic inflammatory response syndrome

Wang

et al. 2023

Front. Immunol.

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Systemic inflammatory response syndrome (SIRS) is a non-specific exaggerated defense response caused by infectious or non-infectious stressors such as trauma, burn, surgery, ischemia and reperfusion, and malignancy, which can eventually lead to an uncontrolled inflammatory response. In addition to the early mortality due to the “first hits” after trauma, the trauma-induced SIRS and multiple organ dysfunction syndrome (MODS) are the main reasons for the poor prognosis of trauma patients as “second hits”. Unlike infection-induced SIRS caused by pathogen-associated molecular patterns (PAMPs), trauma-induced SIRS is mainly mediated by damage-associated molecular patterns (DAMPs) including mitochondrial DAMPs (mtDAMPs). MtDAMPs released after trauma-induced mitochondrial injury, including mitochondrial DNA (mtDNA) and mitochondrial formyl peptides (mtFPs), can activate inflammatory response through multiple inflammatory signaling pathways. This review summarizes the role and mechanism of mtDAMPs in the occurrence and development of trauma-induced SIRS.

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“…Mouse primary alveolar macrophages (AMs) were isolated as previously described (Dong et al, 2021). In brief, mice were killed, and the thoracic cavity and trachea were exposed.…”

Section: Primary Cells and Cell Linesmentioning

confidence: 99%

Chrysophanol alleviates acute lung injury caused by Klebsiella pneumoniae infection by inhibiting pro‐inflammatory cytokine production

Jiang

et al. 2023

Phytotherapy Research

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Acute lung injury (ALI) caused by acute bacterial infection remains a common life‐threatening lung disease. An increased inflammatory response is the basis for the occurrence and development of ALI. Most antibiotics can only reduce the bacterial load but do not protect from lung damage because of an excessive immune response. Chrysophanol (chrysophanic acid, Chr), as a natural anthraquinone extracted from Rheum palmatum L., has various biological functions, including anti‐inflammatory, anti‐cancer activities, and ameliorative effects on cardiovascular diseases. Considering these properties, we investigated the effect of Chr in Klebsiella pneumoniae (KP)‐induced ALI mice and its potential mechanism. Our results showed that Chr had protective effects against KP‐infected mice, including increased survival rate, decreased bacterial burden, reduced recruitment of immune cells, and reduced reactive oxygen species level of lung macrophages. Chr reduced the expression of inflammatory cytokines by inhibiting the toll‐like receptor 4/nuclear factor kappa‐B (TLR4/NF‐κB) signaling pathway and inflammasome activation and strengthening autophagy. Overactivation of the TLR4/NF‐κB signaling pathway by the activator Neoseptin 3 led to Chr losing control of inflammatory cytokines in cells, resulting in increased cell death. Similarly, overactivation of the c‐Jun N‐terminal kinase signaling pathway using the activator anisomycin resulted in Chr losing its inhibitory effect on NOD‐like receptor thermal protein domain associated protein 3 (NFRP3) inflammasome activation, and cell viability was reduced. In addition, autophagy was blocked by siBeclin1, so Chr could not reduce inflammatory factors, and cell viability was markedly inhibited. Collectively, this work unravels the molecular mechanism underpinning Chr‐alleviated ALI via inhibiting pro‐inflammatory cytokines. Thus, Chr is a potential therapeutic agent for KP‐induced ALI.

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Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Cited by 24 publications

References 63 publications

Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

The role of mtDAMPs in the trauma-induced systemic inflammatory response syndrome

Chrysophanol alleviates acute lung injury caused by Klebsiella pneumoniae infection by inhibiting pro‐inflammatory cytokine production

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