Anthocyanin Encapsulated by Ferulic Acid-Grafted-Maltodextrin (FA-g-MD) Microcapsules Potentially Improved its Free Radical Scavenging Capabilities Against H2O2-Induced Oxidative Stress

Abstract

This study aimed to investigate the antioxidant activity and release behavior of anthocyanin (ANC) loaded within FA-g-MD wall (ANC-FA-g-MD microcapsule) in vitro. The microencapsulation of ANC was prepared by spray drying and displayed a biphasic release profile. The combination of ANC and FA-g-MD (0.0625–1 mg/mL) showed a higher antioxidant activity than that of both individuals. A possible intermolecular interaction between ANC and FA-g-MD was studied by UV-vis spectra. Intracellular reactive oxygen species (ROS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test, and protein expression of quinone oxidoreductase 1(NQO1), glutathione reductase (GSR) and γ-glutamate cysteine ligase catalytic subunit (γ-GCLC) were measured through human colon cancer cells (HT-29). After a 24-hour incubation of the HT-29, the combinations (0–60 μg/mL) exhibited a high potential to diminish the ROS level. And the distinct upregulated expressions of GCLC and NQO1 of HT-29 were detected after treatment with combinations compared to those of single ones. These results suggested that the ANC-FA-g-MD microcapsules exerts enhanced antioxidant effect with capability of the modulation of GCLC and NQO1.

Anthocyanins in Colorectal Cancer Prevention Review

“…The biological properties, especially the antioxidant effects, of bilberry extract-loaded pectin amide core-shell capsules, whey protein matrix capsules, and coated apple pectin matrix capsules were comparable to those of the non-encapsulated bilberry extract [108]. In colon cancer cell line HT-29, anthocyanins encapsulated within the FA-g-MD wall showed higher antioxidant activities than anthocyanins alone [109]. Endogenous nanoparticle exosomes can be used to encapsulate anthocyanins in order to increase their availability and efficiency.…”

A technical strategy to prolong anthocyanins thermal stability in formulated purple potato (Solanum tuberosum L. cv Bora valley) processed by hot‐melt extrusion

“…The oxidation of anthocyanins occurred in the aqueous media for F0 and F1. Biopolymer was reported to create a physical barrier that protects anthocyanins from degradation (Ma et al ., 2019b). Our research showed that the lecithin biopolymer significantly prevents anthocyanins from oxidation.…”

Sulforaphane: Expected to Become a Novel Antitumor Compound

“…To overcome the instability of SFE at high temperatures, some researchers have developed hydroxypropyl-β-cyclodextrin (HP-β-CD) and maltodextrin (MD) microcapsules loaded with SFE 71 . As ROS-induced oxidative stress has been shown to be involved in the pathogenesis of many diseases, a recent study showed that MD microcapsules can increase the antioxidant capacity of natural compound anthocyanins and reduce ROS levels 72 . This suggests that HP-β-CD and MD microcapsules containing SFE might also have similar potential and need to be further clarified in other clinical applications.…”