ANTHER-DERIVED CALLUS FORMATION IN BITTER MELON (Momordica charantia L.) AS INFLUENCED BY MICROSPORE DEVELOPMENT STAGE AND MEDIUM COMPOSITION

Nguyen, Minh Tien; Ta, Triseleva; Huyen, T.N.B.T.; Voronina, A.V.

doi:10.15389/agrobiology.2019.1.140eng

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…Statistical analyses revealed that there is a strong relationship between microspore diameter, bud size, bud index, sepal position, and petal colour and their corresponding microspore development phase and depicted that bud morphology is an indicator and a convenient parameter to predict the appropriate microspore development stage, and could be used reliably in anther culture of citron watermelon. Similar results were reported for anther/microspore culture in many crops [3,7,17,22,23,34,41].…”

Section: Resultssupporting

confidence: 85%

“…The sepals were quite small, hardly visible, and sometimes deformed. The average size of citron microspores and pollen grains is considerably narrower than squash, pumpkin, and winter squash [13] but similar to melon [23].…”

Section: Austin Publishing Groupmentioning

confidence: 85%

“…It is well known that microspores at the uninucleate stage are generally favorable for the anther culture for many species and genotypes. But it differs among species and genotypes from tetrad, early to late uninucleate to the binucleate stage [3,9,23,27,30,39]. Because of many limitations, the anther culture-based double haploidization process is not generated in citron watermelon as a recalcitrant species.…”

Section: Introductionmentioning

confidence: 99%

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Determination of the Microspore Development Concerning Floral Morphology for Improving Drought Tolerant Citron Watermelon (Citrullus lanatus var. citroides) Rootstocks Via Androgenesis.

ES,

et al. 2023

annagriccropsci

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The negative effects of abiotic stress factors (especially drought and salinity) that arise due to global climate change are increasing in today’s agricultural production. To eliminate the negative effects of these stress factors, some cultural practices have been implemented such as the breeding of tolerant cultivars and rootstock, exogenous applications of PGPR, AMF, and chemical substances, etc. Contrary to exogenous applications, providing tolerance with breeding methods has an important role in efficient and sustainable agriculture. Androgenesis is a phenomenon for the induction and development of microspore-derived pure lines, as initial material for F1 cultivar breeding, in different vegetable crops. Success in microspore embryogenesis is considerably related to the culture of microspores at an appropriate developmental stage. There is no standard protocol for the anther culture in citron watermelon (Citrullus lanatus var. citroides). Thus, a relation between floral morphology (bud length and width, bud index, sepal position, petal color), and microspore development (microspore diameter, uninucleated and binucleated phases) was investigated in citron. The male flower buds were collected at different sizes and microspore development was observed in both light and fluorescence microscopy. A strong positive relationship was detected between flower bud morphology and the specific stages of microspore development. To the author’s knowledge, this is the first report to indicate that the microspore developmental stage can be estimated by flower bud morphology, and applied in the anther/microspore culture of citron watermelon.

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Section: Resultssupporting

confidence: 85%

Section: Austin Publishing Groupmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Determination of the Microspore Development Concerning Floral Morphology for Improving Drought Tolerant Citron Watermelon (Citrullus lanatus var. citroides) Rootstocks Via Androgenesis.

ES,

et al. 2023

annagriccropsci

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“…The microspore development stage is the first factor to be accounted for when applying the isolated microspore culture technique to a new culture, because the development from tetrads to two-celled pollen may involve different stages (Touraev et al, 1991;Germanà, 2011). For example, to produce carrot DHs, it is recommended to cultivate tetrads and early microspores (Gorecka et al, 2010), while cultivation of middle and late microspores is most effective for callus induction in the balsam apple anther culture (Nguyen et al, 2019). And in the cabbage family, vacuolated microspores and two-celled pollen have the highest ability for embryogenesis (Telmer et al, 1992 , 1997;Custers et al, 2001;Babbar et al, 2004;Winarto, Teixeira da Silva, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…As a rule, plant material is se lected based on such markers as the morphological characteristics of the flower buds and anthers (Takahata, Keller, 1991;Parra-Vega et al, 2013). In rape, soya, reddish, tomato, balsam apple, these markers include the length and widths of their flower buds (Weber et al, 2005;Han et al, 2014;Sumarmi et al, 2014;Adhikari, Kang, 2017;Nguyen et al, 2019). Several studies have proved that such parameters as the size and color of the flower cup as well as the cup/corolla length ratio and anther size can do the trick (De Moraes et al, 2008;Parra-Vega et al, 2013;Zhang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Association of bud and anther morphology with developmental stages of the male gametophyte of melon (Cucumis melo L.)

et al. 2022

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Correlations between the morphological features of f lower buds and the developmental stages of the male gametophyte are of great practical interest as a reliable marker that accelerates and simplif ies the selection of appropriate plant material for isolated microspore culture. Microspore culture enables one to quickly obtain many pure lines of different vegetable crops, but it has not yet been widely applied in the melon (Cucumis melo L.). To successfully apply this technique in a new culture, one has to optimize many of its elements: f irst, f ind the biological markers for selecting the f lower buds containing the microspores of certain development stages. The paper presents the results of research estimating the correlations between the length and diameter of the f lower buds, the length of the visual part of the corolla, the length of the anthers and the development stages of the male gametophyte in the F1 hybrid of the Kim Hong Ngoc melon. The strongest correlation (CC = 0.885) was found for the f lower bed diameter and a strong correlation (CC = 0.880), for the bud length. The corolla’s visual part was a less reliable morphological feature, and the anther’s length should not be used as a parameter to predict the developmental stages of the melon’s male gametophyte. It was also found that one anther could contain the microspores and pollen grains of different developmental stages. In the f lower buds less than 4 mm in length and 1.51 ± 0.02 mm in diameter prevailed tetrads, and in the buds 4.0–4.9 mm in length and 2.30 ± 0.02 mm in diameter, early microspores. The microspores of a middle stage of development prevailed in the f lower buds 5.0–5.9 mm in length and 2.32 ± 0.00 mm in diameter; mid and late vacuolated microspores, in the buds 6.0–8.9 mm in length and 2.96 ± 0.37 mm in diameter; and two-celled pollen, in the buds more than 9 mm in length and more than 3.97 ± 0.34 mm in diameter.

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Recent Advances in Breeding of Bitter Gourd (Momordica charantia L.)

Rao

2021

Advances in Plant Breeding Strategies: Vegetable Crops

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ANTHER-DERIVED CALLUS FORMATION IN BITTER MELON (Momordica charantia L.) AS INFLUENCED BY MICROSPORE DEVELOPMENT STAGE AND MEDIUM COMPOSITION

Cited by 6 publications

References 22 publications

Determination of the Microspore Development Concerning Floral Morphology for Improving Drought Tolerant Citron Watermelon (Citrullus lanatus var. citroides) Rootstocks Via Androgenesis.

Determination of the Microspore Development Concerning Floral Morphology for Improving Drought Tolerant Citron Watermelon (Citrullus lanatus var. citroides) Rootstocks Via Androgenesis.

Association of bud and anther morphology with developmental stages of the male gametophyte of melon (Cucumis melo L.)

Recent Advances in Breeding of Bitter Gourd (Momordica charantia L.)

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