Anther culture of Solanum tuberosum L.

Dunwell, J. M.; Sunderland, N.

doi:10.1007/bf00022640

Cited by 96 publications

(23 citation statements)

References 7 publications

(7 reference statements)

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“…Seasonal variations in anther response were reported for a number of species including Solanum tuberosum (4), Hordeum vulgare (2), and Triticum aestivum (17). For B. napus, controlled studies of the effects of growth temperature, light intensity, and light duration on anther culture response showed that embryo production could be significantly increased by growing the donor plants at a low (1 5C) temperature (3,11,12) and high light (12,20).…”

mentioning

confidence: 99%

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Pauls²

1992

Plant Physiol.

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The influence of donor plant growth conditions on microspore embryogenesis in rapeseed (Brassica napus) was metric measurements of the microspores isolated from 2.5-to 5.0-millimeter buds showed that the microspores isolated from low-temperature-grown plants had significantly lower log 90-degree light scatter to forward angle light scatter and log 90-degree light scatter to time of flight ratios than those isolated from high-temperature-grown plants, suggesting that the former are more translucent than the latter. Thus, the effect of donor plant growth temperature on microspore embryogenesis may be mediated by a change in the physiology of the microspore cell, which results in the reduction of its cytoplasmic granularity and/ or exine density.Haploid plants can be produced by culturing anthers or isolated microspores of rapeseed (Brassica napus) (3, 9, 12, 13). Microspore to haploid embryo conversion frequencies as high as 10 to 50% have been reported (9, 16), but for most genotypes <1% of the microspores that are cultured will develop into embryos.Previous research with several plant species has shown that the conditions under which the donor plants were grown affect the frequency of androgenesis in culture. Seasonal variations in anther response were reported for a number of species including Solanum tuberosum (4), Hordeum vulgare (2), and Triticum aestivum (17). For B. napus, controlled studies of the effects of growth temperature, light intensity, and light duration on anther culture response showed that embryo production could be significantly increased by growing the donor plants at a low (1 5C) temperature (3,11,12) and high light (12,20). There is no detailed report of the 'Supported by the Ontario Ministry of Agriculture and Food.

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confidence: 99%

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Pauls²

1992

Plant Physiol.

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“…In only a few cases (5,10,14,20) has plant regeneration been achieved from excised tissues other than shoot tips. Recently, however, there has been definite progress toward the development of an in vitro regeneration system from single cells.…”

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confidence: 99%

Mesophyll Cell Protoplasts of Potato

Shepard¹,

Totten²

1977

Plant Physiol.

250

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Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves (e.g. 2, 6, 8, 9) have been induced to reform their cell walls, undergo sustained proliferation, and ultimately redifferentiate whole plants. This feature of protoplast totipotency among the experimental plant species studied thus far suggests a viable new approach toward crop and varietal improvement (3) once similar success is achieved for species of major economic consequence.The potato, which ranks fourth among world food crops (19), has been recalcitrant in tissue culture. In only a few cases (5, 10, 14, 20) has plant regeneration been achieved from excised tissues other than shoot tips. Recently, however, there has been definite progress toward the development of an in vitro regeneration system from single cells. Upadhya (18) cultured protoplasts from potato leaves and obtained calli which differentiated roots but not shoots. Behnke (1) plated cells from dihaploid suspension cultures and reported that "small shoots" emerged in a high percentage of individual calli, although mention was not made of whether whole plants were obtained. In the present study, calli raised from single mesophyll protoplasts of potato were induced to undergo shoot formation and eventually regenerate whole plants. The developmental sequence, while dependent upon the proper balance of phytohormones, was also sensitive to numerous other constituents of the culture medium.

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“…4-5). In any case the cell walls were not as distinct as those observed in the case of Nicotiana (NITSCH, 1972;SUNDERLAND, 1971), Datura (IYER & RAINA, 1972) and in vitro formed embryoids of Solarium (DUNWELL & SUNDERLAND, 1973).…”

Section: Resultsmentioning

confidence: 90%

The origin and in vivo development of embryoids in the anthers of Solanum hybrids

Ramanna

1974

Euphytica

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Anther culture of Solanum tuberosum L.

Cited by 96 publications

References 7 publications

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Mesophyll Cell Protoplasts of Potato

The origin and in vivo development of embryoids in the anthers of Solanum hybrids

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