Anther culture induces transposable element movement in potato

Lu, Nan; Zhao, Bingyu; Pereira, Andy; Shulaev, Vladimir; Veilleux, Richard E.

doi:10.1007/s11240-014-0576-3

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…Breeding approaches in vitro, including somatic hybridization, represent a potential resource of somaclonal variation 13 , 14 , which likely extends diversity in clonal progenies. Practical experiences and the value of somaclonal variation for potato breeding 15 , and the most prominent genetic conditions of somaclonal variation 16 were reviewed.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic, molecular and biochemical evaluation of somatic hybrids between Solanum tuberosum and S. bulbocastanum

Sedlák

Sedláková

Vašek

et al. 2022

Sci Rep

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Somatic hybridization has been frequently used to overcome sexual incompatibility between potato and its secondary germplasm. The primary objective of this study was to produce and evaluate somatic hybrids of Solanum tuberosum (Stub) and S. bulbocastanum (Sblb) for breeding purposes. In 2007, 23 somatic hybrids were produced using an electrofusion of mesophyll protoplasts of diploid (2n = 2x = 24) potato line StubDH165 and S. bulbocastanum PI24351 (Sblb66). Phenotype of somatic hybrids in field conditions were evaluated, together with constitution and stability of 30 nuclear (ncSSR) and 27 cytoplasmic (cpSSR) microsatellite markers and content of main glycoalkaloids. All somatic hybrids had very high field resistance against late blight, but the plants were infertile: the viability of pollen grains insignificantly varied between 0.58 and 8.97%. A significant somaclonal variation was observed in terms of the morphology of plants, the date of emergence, the quantity of harvested tubers, the content of glycoalkaloids in foliage, and nuclear microsatellite markers (ncSSR). The analysis of ncSSR identified five distinct genotypes of hybrids partly associated with phenotype variations. The process of somatic hybridization with regeneration of shoots was identified as the most likely source of somaclonal variation because the ncSSR genotypes of hybrids, which were maintained in vitro, remained stable for more than 10 years. The infertile somatic hybrids have no practical breeding potential, but they are considered very suitable for advanced studies of the differential expression of genes in the pathways linked to dormancy of tubers and synthesis of glycoalkaloids.

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Section: Introductionmentioning

confidence: 99%

Phenotypic, molecular and biochemical evaluation of somatic hybrids between Solanum tuberosum and S. bulbocastanum

Sedlák

Sedláková

Vašek

et al. 2022

Sci Rep

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“…The pCAMBIA0380 vector (6812 bp) was derived from the pPZP vector (Abcam, 2021). The pCAMBIA0380 vector has been used to transform plants such as rice, potato and fungus (Lu et al, 2015;Oliva et al, 2014;Sayari et al, 2019;Zhou et al, 2013). Both pBINPLUS/ARS and pCAMBIA0380 differ in size, backbone and origin of replication elements for replication in Agrobacterium (Abcam, 2021;Belknap et al, 2008).…”

Section: Evaluation Of Transformation Vectors In Oil Palm Callimentioning

confidence: 99%

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

AYUB¹

2023

JOPR

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Current oil palm transformation efficiency was relatively low compared to other monocots such as rice and sorghum. Thus, the construction of efficient transformation vectors is crucial for Agrobacterium-mediated oil palm genetic engineering to elevate the transformation efficiency. In this study, six transformation vectors, which contain phosphinothricin acetyltransferase (bar), modified green fluorescent protein (mgfp) and synthetic green fluorescent protein (sgfp(S65T)) genes driven by either maize ubiquitin (Ubi-1) or cauliflower mosaic virus (CaMV35S) promoters, were assembled in pBINPLUS/ARS or pCAMBIA0380 backbones. The efficiency of these vectors was evaluated in oil palm calli. Based on transient GFP signals, the pBINPLUS/ARS-based vectors produced a higher number of GFP signals than the pCAMBIA0380-based vectors. The CaMV35S promoter was more excellent than the Ubi-1 promoter in driving the expression of gfp genes. Furthermore, the sgfp(S65T) variant was more suitable for oil palm transformation than the mgfp variant because it produced more GFP signals in the oil palm calli than the mgfp variant. These results suggested that the pBAR65 vector, which carries the bar and sgfp(S65T) genes driven by the CaMV35S promoter in the pBINPLUS/ARS backbone, is the most suitable vector and could be used to develop an efficient Agrobacterium-mediated transformation system for oil palm.

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Anther culture induces transposable element movement in potato

Cited by 3 publications

References 21 publications

Phenotypic, molecular and biochemical evaluation of somatic hybrids between Solanum tuberosum and S. bulbocastanum

Phenotypic, molecular and biochemical evaluation of somatic hybrids between Solanum tuberosum and S. bulbocastanum

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

Encapsulation-based a novel antibiotic selection technique for Agrobacterium-mediated genetic transformation of Dendrobium Broga Giant orchid

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