Anther-Based Regeneration of African Violet (Saintpaulia ionantha)

Uno, Yuichi; Yagi, Masafumi

doi:10.1007/978-1-0716-1331-3_16

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…Auxin alone or in combination with cytokinin was exhibited to stimulate shoot generation of plants such as Sinningia speciosa [16] and Saintpaulia [27][28][29]. In our experiments, the addition of NAA significantly improved the number and proliferation coefficient of adventitious shoots.…”

Section: Effect Of Pgrssupporting

confidence: 48%

“…Moreover, in vitro leaf explants are more suitable for genetic transformation research as they are sterile, and gene stacking technology is feasible [16,36]. Many studies have successfully established the tissue culture system using leaves as explants, such as Sinningia speciosa [16] and Saintpaulia species [27][28][29]. In our study, both field-grown leaves and aseptic seeding leaves as explants successfully induced shoot regeneration from S. hybrida by organogenesis pathway.…”

Section: Leaf As Explant Source For Regenerationmentioning

confidence: 67%

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Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Yang

Wang

et al. 2022

Plants

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As a valuable ornamental plant, Sinningia hybrida ‘Isa’s Murmur’ (S. hybrida) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approach for the sustainable propagation of S. hybrida. Compared with aseptic seeding leaves explants, field-grown leaves explants are more suitable for adventitious shoot regeneration. Adding 0.1 mg L−1 NAA and 2.0 mg L−1 TDZ could obtain the highest adventitious shoot proliferation coefficient (24.5), and the induction rate was 91.7%. The shoot proliferation coefficient (20.7) and the greatest shoot length and induction rate (95.3%) were achieved in 0.1 mg L−1 NAA and 2.0 mg L−1 BA medium, accompanied by rooting formation. Adding 0.5 mg L−1 GA3, 1.0 mg L−1 BA, and 0.2 mg L−1 IBA to MS medium can effectively prolong the regenerated buds for rooting. The best for rooting was 1/2 MS medium containing 0.3 mg L−1 IBA, with the maximum number of roots (13.4 per shoot) and survival rate for transplanting (100%). This work aims to build an efficient, definitive, and scalable protocol for S. hybrida regeneration useful for large-scale cultivation and even more protoplast fusion and genetic transformation to develop more colorful or fragrant flowers.

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Section: Effect Of Pgrssupporting

confidence: 48%

Section: Leaf As Explant Source For Regenerationmentioning

confidence: 67%

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Yang

Wang

et al. 2022

Plants

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“…Furthermore, it appears that at certain concentrations, callus formation may be induced while direct shoot organogenesis is impeded [ 31 ]. Similar observations have been made in other members of the Gesneriaceae family, including A. radicans [ 2 ], Saintpaulia ionantha [ 32 ], and Lysionotus serratus D. Don [ 33 ]. Here, we found that the application of the auxin NAA was particularly beneficial to the formation of callus structures.…”

Section: Discussionsupporting

confidence: 80%

Development of a Highly Efficient Shoot Organogenesis System for an Ornamental Aeschynanthus pulcher (Blume) G. Don Using Leaves as Explants

Yang

Yuan

et al. 2022

Plants

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Aeschynanthus pulcher (Blume) G. Don, the “lipstick plant” is a prized ornamental plant with distinctive flowers. Here, we introduce a novel in vitro regeneration method for A. pulcher using leaf explants and an optimized combination of phytohormone plant growth regulators (PGRs). The optimal conditions for shoot regeneration included 1 mg L−1 polyvinyl pyrrolidone (PVP) plus 3 mg L−1 thidiazuron (TDZ), inducing a response rate of 82.4% and a shoot/explant ratio of 38.6. When the Murashige and Skoog (MS) medium contained indole-3-butyric acid (IBA) alone, leaves first differentiated into adventitious roots and then adventitious shoots. Leaves cultured on MS medium containing 1 g L−1 PVP, 3 mg L−1 TDZ, 5 mg L−1 casein, and 0.1 mg L−1 α-naphthaleneacetic acid (NAA) for 30 d exhibited the highest embryogenic callus (EC) induction rate (95.6%). The optimal shoot proliferation coefficient (21.5) was obtained when shoots derived from EC were cultured on the same medium as that used for EC induction for 5 weeks. The most effective medium for rooting of elongated shoots was MS medium containing 1 g L−1 PVP, 5 mg L−1 casein, 3 mg L−1 6-benzyladenine (BA), and 0.1 mg L−1 NAA, and the number of roots reached 18.8. The regenerated plants grown in a greenhouse had 100% survival following one week of hardening. Overall, our effective and efficient propagation method should result in shortened culture periods and reduced production costs, allowing for the future selective breeding and genetic improvement of A. pulcher.

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Anther-Based Regeneration of African Violet (Saintpaulia ionantha)

Cited by 2 publications

References 13 publications

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Development of a Highly Efficient Shoot Organogenesis System for an Ornamental Aeschynanthus pulcher (Blume) G. Don Using Leaves as Explants

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