Anthelmintic resistance in ovine gastrointestinal nematodes in inland southern <scp>Q</scp>ueensland

Lyndal-Murphy, M.; Ehrlich, WK; Mayer, David G.

doi:10.1111/avj.12250

Cited by 16 publications

(12 citation statements)

References 23 publications

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“…Fifty years have passed since the earliest reports of anthelmintic resistance in sheep ( Drudge et al, 1964 ; Smeal et al, 1968 ) and the problem now has grown and spread to all continents, occurring in multiple nematode species and against multiple classes of drugs ( Chandrawathani et al, 2003 ; Čerňanská et al, 2006 ; Domke et al, 2012 ; Papadopoulos et al, 2012 ; Falzon et al, 2013 ; Chandra et al, 2014 ; Geurden et al, 2014 ; Lyndal-Murphy et al, 2014 ; Rose et al, 2015 ; Learmount et al, 2016a ; Gárcia et al, 2016 ; Salgado and Santos, 2016 ; Ploeger and Everts, 2018 ). However, the methods used to diagnose anthelmintic resistance have changed very little and predominantly rely on determining the reduction of fecal egg counts following anthelmintic treatment.…”

Section: Introductionmentioning

confidence: 99%

The use of ITS-2 rDNA nemabiome metabarcoding to enhance anthelmintic resistance diagnosis and surveillance of ovine gastrointestinal nematodes

Queiroz

Levy

Avramenko

et al. 2020

International Journal for Parasitology: Drugs and Drug Resistan

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(300 words) A lack of quantitative information on the species composition of parasite communities present in fecal samples is a major limiting factor for the sensitivity, accuracy and interpretation of the diagnostic tests commonly used to assess anthelmintic efficacy and resistance. In this paper, we investigate the ability of ITS-2 rDNA nemabiome metabarcoding to enhance fecal egg count reduction testing by providing information on the effect of drug treatments on individual parasite species. Application of ITS-2 rDNA nemabiome metabarcoding to fecal samples from ewes from over 90 flocks across western Canada revealed high gastrointestinal nematode infection intensities in many flocks with Haemonchus contortus being the most abundant species followed by Teladorsagia circumcincta and then Trichostrongylus colubriformis . Integration of ITS-2 rDNA nemabiome metabarcoding with pre- and post-treatment fecal egg counting revealed consistently poor efficacy of producer-applied ivermectin and benzimidazole treatments against H. contortus, but much better efficacy against T. circumcincta and T. colubriformis , except for in a small number of flocks. Integration of nemabiome ITS-2 rDNA metabarcoding with Fecal Egg Count Reduction Tests (FECRT), undertaken on farm visits, confirmed that ivermectin and fenbendazole resistance is widespread in H. contortus but is currently less common in T. circumcincta and T. colubriformis in western Canada . FECRT/nemabiome testing did not detect moxidectin resistance in any GIN species but suggested the early emergence of levamisole resistance specifically in T. circumcincta. It also revealed that although poor efficacy to closantel was relatively common, based on total fecal egg counts, this was due to its narrow spectrum of activity rather than the emergence of anthelmintic resistance. This study illustrates the value of ITS-2 rDNA nemabiome metabarcoding to improve fecal egg count resistance testing, perform large-scale anthelmintic resistance surveillance and direct more targeted rational anthelmintic use.

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Section: Introductionmentioning

confidence: 99%

The use of ITS-2 rDNA nemabiome metabarcoding to enhance anthelmintic resistance diagnosis and surveillance of ovine gastrointestinal nematodes

Queiroz

Levy

Avramenko

et al. 2020

International Journal for Parasitology: Drugs and Drug Resistan

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“…The most recently introduced group of anthelmintics, the amino-acetonitrile derivatives, have been no exception with resistance to the drug monepantel reported in the Netherlands just three years after its introduction (Van den Brom et al, 2015). One strategy used to overcome resistance in Australia is combination drug therapy, however reports of multi-drug resistance further complicate managing infections (Lyndal-Murphy et al, 2014). New drugs are urgently needed to ensure sheep welfare and the continued sustainability of the Australian sheep industry.…”

Section: Introductionmentioning

confidence: 99%

The antitrypanosomal diarylamidines, diminazene and pentamidine, show anthelmintic activity against Haemonchus contortus in vitro

Nixon

Saez

Herzig

et al. 2019

Veterinary Parasitology

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“…The results of an observational parasitological study in southern Queensland confirm widespread resistance in Haemonchus contortus populations in that region …”

Section: Production Animalsmentioning

confidence: 61%

In this issue – November 2014

Jackson¹,

Cabral²

2014

Aust Veterinary J

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Anthelmintic resistance in ovine gastrointestinal nematodes in inland southern Queensland

Abstract: This level of anthelmintic resistance in southern Queensland will severely compromise worm control and force increased use of monepantel.

Cited by 16 publications

References 23 publications

The use of ITS-2 rDNA nemabiome metabarcoding to enhance anthelmintic resistance diagnosis and surveillance of ovine gastrointestinal nematodes

The use of ITS-2 rDNA nemabiome metabarcoding to enhance anthelmintic resistance diagnosis and surveillance of ovine gastrointestinal nematodes

The antitrypanosomal diarylamidines, diminazene and pentamidine, show anthelmintic activity against Haemonchus contortus in vitro

In this issue – November 2014

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