Anthelmintic efficacies of three common disinfectants and extracts of four traditional Chinese medicinal plants against Gyrodactylus kobayashii (Monogenea) in goldfish (Carassius auratus)

Zhou, Shun; Li, Wen X.; Wang, Ya Q.; Zou, Hong; Wu, Shan; Wang, Gui T.

doi:10.1016/j.aquaculture.2016.09.048

Cited by 38 publications

(18 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given the harmful impacts on aquatic animals, the environment and human health caused by the overuse of antibiotics and other disease control chemicals (Cabello et al, ; Howard, ; Lazado, Caipang, & Estante, ; Meidong et al, ; Miranda, Tello, & Keen, ; Smith, Harris, Johnson, Silbergeld, & Morris, ), there is an urgent need for the aquaculture industry to find more effective and sustainable reagents to control the diseases. Medicinal plants provide an alternative solution because of their demonstrated medical efficacy and environmental friendliness (Rahuman, Gopalakrishnan, Venkatesan, & Geetha, ; Zhou et al, ).…”

Section: Introductionmentioning

confidence: 99%

The effects of dietaryEucommia ulmoidesOliver on growth, feed utilization, antioxidant activity and immune responses of turbot (Scophthalmus maximusL.)

Zhang

Wang

et al. 2018

Aquacult Nutr

View full text Add to dashboard Cite

The present study was conducted to evaluate the effects of dietary Eucommia ulmoides (EU) on growth, feed utilization, antioxidant activity and immune responses of turbot fed a basal diet (CON) or EU‐supplemented diets with 5.0 g/kg (EU1), 10.0 g/kg (EU2) and 20.0 g/kg (EU3) EU leaf powder. After 70‐day trial, EU supplementation did not affect nutrient utilization, but reduced feed intake (FI) and specific growth rate (SGR) of fish at doses above 5.0 g/kg. Superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (T‐AOC) activities in the EU‐supplemented groups were significantly higher than those in the control group at a dose of 20.0 g/kg. Significantly reduced malondialdehyde (MDA) contents were observed in EU‐supplemented groups at doses over 5.0 g/kg. Furthermore, fish fed 20.0 g/kg EU showed the highest lysozyme (LZM) activity among groups. The EU‐supplemented diets with doses above 5.0 g/kg significantly enhanced the mRNA expressions of cytokines. The expression level of major histocompatibility complex II alpha (MHC IIα) was significantly upregulated compared to that of the control fish when the supplemental level was at 20.0 g/kg. Taken together, the present study indicated that the EU could remarkably enhance the antioxidant activity, non‐specific immunity and maintain an active immune response in turbot.

show abstract

Section: Introductionmentioning

confidence: 99%

The effects of dietaryEucommia ulmoidesOliver on growth, feed utilization, antioxidant activity and immune responses of turbot (Scophthalmus maximusL.)

Zhang

Wang

et al. 2018

Aquacult Nutr

View full text Add to dashboard Cite

show abstract

“…The number of gyrodactylids on both sides of the caudal fin was counted under a stereomicroscope in 0, 24 h and 48 h post treatment. Anthelmintic efficacy of compound 2 was calculated according to the methods of Zhou et al [30]. In vitro assays were performed to detect the anthelmintic activity of GNP-6 and GNP-10.…”

Section: Anthelmintic Activitymentioning

confidence: 99%

Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites

Tan

et al. 2020

J Nanobiotechnol

View full text Add to dashboard Cite

Gold nanoparticles (GNPs) are one of the most widely used nanomaterials in various fields. Especially, the unique chemical and physical properties make them as the promising candidates in drug target identification, unfortunately, little is known about their application in parasites. In this paper, GNPs were employed as new solid support to identify drug targets of natural bioactive compound arctigenin (ARG) against fish monogenean parasite Gyrodactylus kobayashi. Before target identification, GNPs with ARG on the surface showed the ability to enter the live parasites even the nucleus or mitochondria, which made the bound compounds capable of contacting directly with target proteins located anywhere of the parasites. At the same time, chemically modified compound remained the anthelminthic efficacy against G. kobayashii. The above results both provide assurance on the reliability of using GNPs for drug target-binding specificity. Subsequently, by interrogating the cellular proteome in parasite lysate, myosin-2 and UNC-89 were identified as the potential direct target proteins of ARG in G. kobayashii. Moreover, results of RNA-seq transcriptomics and iTRAQ proteomics indicated that myosin-2 expressions were down-regulated after ARG bath treatment both in transcript and protein levels, but for UNC-89, only in mRNA level. Myosin-2 is an important structural muscle protein expressed in helminth tegument and its identification as our target will enable further inhibitor optimization towards future drug discovery. Furthermore, our findings demonstrate the power of GNPs to be readily applied to other parasite drugs of unknown targets, facilitating more broadly therapeutic drug design in any pathogen or disease model.

show abstract

“…SCXK(AWADCNI)2018-0001). These goldfish were fed and treated to remove all ectoparasites, as described previously [13] . A goldfish experimental infection model using G. kobayashii was performed according to a method described previously [15] .…”

Section: Establishment Of a Goldfish-g Kobayashii Model And Animal Ementioning

confidence: 99%

“…The fourteen Chinese medicinal herbs listed in Table 1 were purchased from a drug store and prepared according to a method described previously [13] . The herbal medicines were washed thoroughly, air-dried and oven-dried at 45°C for 48 h. In order to ensure the complete removal of water, dry plant materials were powdered, strained using a filter net of 30-40 mesh (450-600 µm), and freezedried at -54°C.…”

Section: Preparation Of Plant Materialsmentioning

confidence: 99%

“…Recently, increasing attention has been paid to the use of traditional plantbased medicines to control diseases in aquaculture [11,12] . Zhou et al [13] showed that herbal medicines were able to control Gyrodactylus kobayashii (G. kobayashii) infection in goldfish. Huang et al [14] screened plant extracts with anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Japon Balıklarında (Carassius auratus) Gyrodactylus kobayashii’ye (Monogenea) Karşı Çin Şifalı Otlarının Anthelmintik Etkinliklerinin İncelenmesi

Lian¹,

Zhang²,

Zhou³

et al. 2019

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Monogenean infection can cause high mortality and significant financial losses in commercially farmed fish. Existing chemical drugs for the treatment of such infections often have serious drawbacks. In order to find alternative agents of chemical drugs, fourteen medicinal plants were tested for their in vivo anthelmintic activity against Gyrodactylus kobayashii in goldfish (Carassius auratus). Ethanol extracts of Evodia rutaecarpa, Cnidium monnieri and Sophora flavescens had 100% anthelmintic efficacy at low concentrations (100, 100 and 300 mg/L, respectively), after 48 h of exposure. The ethyl acetate extract of C. monnieri was the most effective, with an EC50 value of 11.0 mg/L, after 48 h of exposure, showing 100% anthelmintic efficacy against G. kobayashii at 50.0 mg/L. Higher anthelmintic activity was also observed for remaining extracts of C. monnieri and E. rutaecarpa except for the water extracts of the two plants. The ethyl acetate extract of C. monnieri had the highest therapeutic index (TI, LC50/ EC50) value of 31.8, and the lowest EC50, which indicates that this extract was the safest to goldfish among all extracts. For the remaining extracts of C. monnieri and E. rutaecarpa, the 48-h LC50 values were about 10-fold higher than the corresponding EC50 values. This result indicates these extracts have low toxicity in goldfish. The ethyl acetate extract of C. monnieri was the most effective and the safest among the tested extracts. Therefore, the two plants are expected to be alternative agents to control monogenean infection.

show abstract

Anthelmintic efficacies of three common disinfectants and extracts of four traditional Chinese medicinal plants against Gyrodactylus kobayashii (Monogenea) in goldfish (Carassius auratus)

Cited by 38 publications

References 37 publications

The effects of dietaryEucommia ulmoidesOliver on growth, feed utilization, antioxidant activity and immune responses of turbot (Scophthalmus maximusL.)

The effects of dietaryEucommia ulmoidesOliver on growth, feed utilization, antioxidant activity and immune responses of turbot (Scophthalmus maximusL.)

Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites

Japon Balıklarında (Carassius auratus) Gyrodactylus kobayashii’ye (Monogenea) Karşı Çin Şifalı Otlarının Anthelmintik Etkinliklerinin İncelenmesi

Contact Info

Product

Resources

About