Anthelmintic activity of plant aqueous extracts against Panagrellus redivivus in vitro

Abstract

Control of phytonematodes is very hard and requires a combination of techniques to succeed. Alternative control through plant extracts may result in the discovery of nematicide substances. Research aimed at evaluating the effect of 33 plants submitted to aqueous extraction against Panagrellus redivivus in vitro. Concentrations were prepared at 1.25, 2.5, 5, 10, and 20%. Monitoring happened at 0, 6, 12, 24 and 30 hours after preparation. Counting considered dead nematodes subtracted from alive ones. Juveniles were also counted, and extract efficiency was expressed in percentage of control or stimuli. Data were submitted to variance analysis. Significant results got with the Scott-Knott test (5%), and multiple linear regression analysis. Extracts were observed acting as controllers, but also as stimulators to nematode reproduction. The best controlling performance was set by Carica papaya (-66% at 20%; -33.7% at 10%), Euphorbia milii (-37% at 20%), Psychotria carthagenensis (-25.5% at 2.5%), Clusia variegate (-22% at 20%), and Zamioculcas zamiifolia (-21.5% at 20%). Stimulator extracts were Mentha villosa at 10% (+148%) and 2.5% (+131.5%), followed by Aloe vera (+123% at 5%), Schinus molle (+112.5% at 10%), Schefflera arboricola (+93.5% at 5%), C. variegate (+89% at 5%), and S. molle (+88% at 5%). Some extracts kept population stable throughout the experiment, presenting lower control indexes. Besides an additive effect, there was an individual influence of concentration or time on control.

Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

“…Different Agave species such as A. angustifolia Haw., A. americana L., A. tequilana Weber and A. sisalana Perrine exhibited nematicide activity against Panagrellus redivivus, Meloidogyne incognita, Haemonchus spp., Oesophagostomum and Trichostronglyus. Those studies used methanolic and aqueous extracts, which killed nematodes within 24 to 72 h of application [86,[110][111][112]. Another study mentioned that during the administration of A. sisalana Perrine agro-waste on infested animals, no health issues were presented.…”

Integrated Management of Ascochyta Blight on Chickpea Germplasm in Pakistan

“…Blight can be managed by several means like the use of resistant varieties, cultural practices include removal of diseased plants from the field avoiding cultivation in the already infected field, and crop rotation. (Lubian et al, 2019). In Pakistan major part of south Punjab, consist of a desert range where agriculture is based on chickpea cultivation so crop rotation is not applicable in this region.…”

Efficacy of fungicides, plant extracts and biocontrol agents against Ascochyta blight (Ascochyta rabiei) of chickpea (Cicer arietinum L.) under field conditions

“…Recently, several plant extracts, viz., Aloe vera, Magnolia grandiflora and Tagetes erectus etc., have been tested and found effective against many plant diseases (8,9). Plants have secondary metabolites with antifungal activity (10). Similarly, biocontrol agents Chaetomium globosum, Trichoderma viride, Acremonium implicatum have been reported in vitro for their effectiveness against ascochyta blight (11,12).…”