Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

Abstract

Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (−)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.

Pharmacological profiling an abundantly expressed schistosome serotonergic GPCR identifies nuciferine as a potent antagonist

“…The leaves, flowers, seeds and rhizomes of these plants have been broadly used in traditional medicine for treating a spectrum of ailments (Mukherjee et al., 2009). Aporphine compounds isolated from Nelumbo nucifera leaves have been previously shown to have anticestodal profiles, with the mechanistic basis for these effects being ascribed to a free radical scavenging effect (Lin et al., 2014). A more recent study investigating the psychotropic properties of nuciferine demonstrated an affinity for several mammalian serotonergic GPCRs, encompassing action as an inverse agonist at the human 5-HT 7 receptor (Hs.5HT 7 R (Farrell et al., 2016)).…”

“…A clinically well-known example is apomorphine, a synthetic morphine derivative used clinically as an anti-Parkinsonian agent due to efficacy at dopaminergic GPCRs. Several aporphine compounds have previously been demonstrated to impair neuromuscular function in a variety of helminth parasites (Ayers et al., 2007, Chan et al., 2014, Lin et al., 2014), although the molecular target of these drugs in parasites is uncharacterized. The affinity of aporphinoids for mammalian 5-HT GPCRs (Munusamy et al., 2013, Ponnala et al., 2014, Ponnala et al., 2015, Farrell et al., 2016) was especially intriguing to us in this context.…”

Anthelmintic activity of ginger, curcumin, and praziquentel against Raillietina cesticillus (in vitro and in vivo)

“…The anthelmintic efficacy of plant extract may be due to the presence of secondary metabolites particularly the alkaloids (Lin et al 2010). Extracts of several medicinal plants have been tested and proved to be effective against many helminth parasites (Abdel-Ghaffar et al 2011;Li et al 2012;Lin et al 2014).…”

A Comprehensive Review on Chemical Profiling of Nelumbo Nucifera: Potential for Drug Development

“…Anti-bacterial and anti-fungal effect. Lin et al isolated ten aporphine derivatives from N. nucifera and discovered their anthelmintic activities against A. simplex and H. nana (Lin et al, 2014). Moreover, Brindha et al tested the efficacy of hydroethanolic extract of N. Nucifera flower against five important bacterial strains and two fungal strains.…”

“…(Continued)Chang et al, 2016;Duan et al, 2014;Liu et al, 2014b;Lin et al, 2014) 42 7-hydroxydehy dronuciferine (7HDNF)Leaves(Wafo et al, 1999;Wu et al, 2015a) 43 Pronuciferine Seeds, Fruits, Leaves(Wang et al, 1991;Kunitomo et al, 1973;Kashiwada et al, 2005;Li et al, 2014;Ma et al…”