Anthelminthic activity of acetone extract and fractions of Vernonia amygdalina against Haemonchus contortus eggs and larvae

Ademola, Isaiah Oluwafemi; Eloff, Jacobus Nicolaas

doi:10.1007/s11250-010-9727-7

Cited by 42 publications

(30 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A study by Molgaard et al (2001), using water extract of leaves, stem, root and root bark of V. amygdalina were able to kill cestodes of Hymenolepis diminuta after 24 hours of treatment. A recent study by Ademola and Eloff (2011), revealed a significant effects on different development stages of Haemonchus contortus that varied with solvent used for extraction of active ingredient.…”

Section: Discussionmentioning

confidence: 99%

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

Nalule¹,

Mbaria²

2013

ABJNA

View full text Add to dashboard Cite

In-vitro studies were conducted to determine the anthelmintic activity of ethanolic and water extracts of Vernonia amygdalina and Secamone africana used by agro-pastoralists in semi-arid land Uganda. The plant materials were collected from Nakasongola district and extracted using 70% ethanol and water. Efficacy and potency of crude extracts was determined using extracts' serial dilutions ranging 4mg/ml to 64mg/ml parallel to serial dilutions of albendazole ranging: 6.25-100mg/ml in three replicates. Ascaris suum model was used for the assays. Standard phytochemical methods were used for qualitative phytochemical analysis. The plants' extracts caused a dose-dependent motility inhibition with highest concentration of both ethanolic and water extracts of V.amygdalina causing 90% inhibition compared with 100% inhibition caused by albendazole. The corresponding median effective doses of ethanolic, water and albendazole were 5.94mg/ml, 13.70mg/ml and 15.12mg/ml respectively and significantly differed (F (2, 53) =257.43, p=0.001). There was a significant difference in motility inhibition in all dose levels (F (5, 53) = 14.01, P = 0.001; R 2 = 0.93). Similarly, the water and ethanolic extracts of S.africana caused a 93.3% and 80% motility inhibition with median effective doses of 40.08mg/ml and 25.41mg/ml respectively which also significantly differed (F (2, 53) =183.26, p= 0.001). There was also a highly significant difference in motility inhibition in all dose levels (F (5, 53) = 8.00, P = 0.001; R 2 = 0.92). The phytochemical screening revealed presence of tannins, alkaloid, saponins, flavonoids, steroids glycosides, triterpenes, coumarin derivatives, anthocyanocides, anthracenocides, and reducing sugars. In conclusion, V.amygdalina and S.africana are potential sources for novel antihelmintics and the secondary metabolites present justify their ethno-veterinary use.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

Nalule¹,

Mbaria²

2013

ABJNA

View full text Add to dashboard Cite

show abstract

“…Results showed that LAE has an ovicidal effect with doses of 250 and 500 µg/mL, which may be due to the condensed tannins in L. acapulcensis (116.3 g/kg DM; Table 1). Ademola and Eloff (2011), in their study with Vernonia amygadalina acetone extracts, inhibited egg hatching and larval Olmedo-Juárez et al Gea et al, 2011). Thus possible anthelmintic effects of tanniniferous plants may depend on their CT contents as well as on other CT parameters such as the monomeric composition and degree of polymerisation (i.e., average size of tannin molecules).…”

Section: Eggs Hatch and Larval Development Assaymentioning

confidence: 95%

In VitroActivity ofPithecellobium DulceandLysiloma Acapulcensison Exogenous Development Stages of Sheep Gastrointestinal Strongyles

Olmedo-Juárez

Rubio

Arece-García

et al. 2014

Italian Journal of Animal Science

View full text Add to dashboard Cite

An experiment was conducted to evaluate the effects of two lyophilised aqueous extracts of Lysiloma acapulcensis (LAE) and Phitecellobium dulce (PDE) tree leaves on in vitro assessment of hatching of eggs, larval development and migration of gastrointestinal nematodes of sheep using a general linear model. Treatments contained extracts from both species at concentrations of 0, 125, 250 and 500 µg/mL. Both albendazole and levamisole were used at a level of 1% as positive control. The extract of LAE, compared to PDE, showed better inhibition (P<0.05) of egg hatching. Different doses of both the LAE and PDE extracts showed a larvicidal effect (P<0.05) on all larvae exposed to different doses of the extracts. In the larval migration assay, a similar effect with levamisole at doses of 250 and 500 µg/mL occurred with the LAE extract. The extract of P. dulce had a lower larvicidal effect (P<0.05) than levamisole and L. acapulcensis extracts. Using aqueous extracts of both species of L. acapulcensis and P. dulce could be a promising alternative to synthetic anthelmintics as treatments of gastrointestinal nematodes of sheep in organic and conventional production systems under subtropical conditions.

show abstract

“…The roots of V. amygdalina have been used for gingivitis and toothache due to its proven antimicrobial activity from the previous work being done on it [2]. In North America, of the 17 species of Vernonia all have the same effective properties as a blood purifier, uterus toner and helps also in the prevention of atherosclerosis [22,41].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Proximate Analysis of Some Medicinal Leaves

Aborisade¹

2017

CMR

View full text Add to dashboard Cite

Abstract:The proximate analysis and the phytochemical constituents were investigated in Magnifera, Morinda lucida, Parquetina nigrescens, Oscmium gratissimum, Chenopodium ambrosioides and Veronia amygaldalina using standard reference methods. The proximate analysis in % showed that Chenopodium ambrosioides had the highest amount of ash content of 17.30 and moisture content of 89.40 while Magnifera indica had the lowest amount of ash content 12.04 and moisture content 68.40. Veronia amygaldalina had the highest amount of protein 30.02 and carbohydrate 54.00 while Parquetina nigrescens had the least amount of protein 25.06 and carbohydrate 40.23. Parquetina nigrescens had the highest amount of crude fibre of 25.05 while Chenopodium ambrosioides had the highest amount of crude fat 18.22. The moisture content was found to be 89.40%, ash content 17.30, fat 18.22%, protein 30.02%, crude fibre 25.05% and carbohydrate 54.00%. The phytochemical constituents indicated flavonoid 28.58% alkaloid 25.84% and tannin 2.94% in Magnifera indica, Veronia amygaldalina and Morinda lucida respectively. The presence of high secondary metabolites in the leaves are good indication that if the plant is subjected to further research such as identification and characterization of plant, bioactive compounds with strong biological activities may be isolated and novel compounds may also be identified.

show abstract

Anthelminthic activity of acetone extract and fractions of Vernonia amygdalina against Haemonchus contortus eggs and larvae

Cited by 42 publications

References 18 publications

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

In VitroActivity ofPithecellobium DulceandLysiloma Acapulcensison Exogenous Development Stages of Sheep Gastrointestinal Strongyles

Phytochemical and Proximate Analysis of Some Medicinal Leaves

Contact Info

Product

Resources

About