Anterior segment optical coherence tomography in eye injuries

Wylęgała, Edward; Dobrowolski, Dariusz; Nowińska, Anna; Tarnawska, Dorota

doi:10.1007/s00417-008-0937-x

Cited by 55 publications

(25 citation statements)

References 17 publications

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“…The cornea is covered by a squamous epithelium possessing intense regenerative potential, thus enabling fast restoration of the damaged eye surface. Corneal cells are the most external part of the eye and are therefore liable to suffer an injury, and so are very useful in toxicological diagnosis in vitro (15). Currently, due to ease to access, ease of culture and repeatable results, cell lines rather than primary cells isolated from the whole organ are most often used to this end.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests

Paduch¹,

Woźniak²,

Niedziela³

et al. 2014

Balkan Med J

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Herba Euphrasiae (Scrophulariaceae), commonly known as eyebright grass, is a composition of very similar plant species, broadly defined as Euphrasiae officinalis L., which are widely used in folk medicine and homeopathy (1, 2). E. officinalis L. is an annual, herbaceous, semi-parasitic plant which exhibits numerous biological activities, including anti-inflammatory, antioxidant, antimicrobial, anticancer, antifungal, antiviral, hypotensive, hapatoprotector, anti-epileptic, and anticatarrhal activities (3-8). In traditional medicine, E. officinalis L. has also been effectively used for the treatment of eye disorders like conjunctivitis, hordeolum, etc. (1, 5, 9). Eye drops made from E. officinalis L. are considered to be useful and safe in the treatment of ocular allergy symptoms (10). Folk medicine commonly uses eyebright aqueous extracts to treat eye disorders. Additionally, methanolic extracts of Euphrasia genus and its methanolic fractions have been evaluated for their antioxidant and anti-inflammatory activities (3). The biological activity of aqueous tinctures and alcohol extracts are based on the accumulation of highly active compounds like iridoids, flavonoids, phenolic acids or etheric oils (1, 2, 5). We may suppose that E. officinalis L. preparations for the treatment of eye disorders are generally based on their antioxidant, anti-inflammatory and regenerative properties. Moreover, as full extracts are used on the principle of the historical tradition, selective extracts are employed mainly for reason of their specific activity (11). Accordingly, in this study, we analyzed the cytotoxic, antioxidant and immunomodulatory effects of ethanol, ethyl acetate and heptane extracts of E. officinalis L. on human corneal epithelial (10.014 pRSV-T) cells.The aim of this study was to answer the question concerning the variety of biological effects of E. officinalis L. extracts obtained using different solvents by in vitro tests on human corneal cells, and their potential medicinal properties/effects when used in the treatment of eye disorders. MATERIAL AND METHODS Plant materialEuphrasia herbs were harvested from the area of Górki Czechowskie (Lublin, Poland) on a sunny day in September 2009. Background:Euphrasia officinalis L. is an herb traditionally used in folk medicine, mainly in the treatment of eye disorders. Aims: The present study analyzed the activity of three extracts of E. officinalis L. (ethanol, ethyl acetate and heptane) on cultured human corneal epithelial cells (10.014 pRSV-T). Study Design: In vitro study. Methods: Toxicity, free radical scavenging activity and the immunomodulatory effects of the extracts were tested using the thiazolyl blue tetrazolium bromide (MTT) or Neutral Red, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ELISA tests, respectively. Moreover, nitric oxide levels and cytoskeleton architecture were analyzed after corneal cell incubation with the plant extracts. Results: We show that the biological effect depended on both the concentration and the extraction solvent used. Heptane ext...

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Section: Discussionmentioning

confidence: 99%

Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests

Paduch¹,

Woźniak²,

Niedziela³

et al. 2014

Balkan Med J

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“…Technology including computed tomography, magnetic resonance imaging, ultrasonography, [13, 14] optical coherence tomography (AS-OCT) [15], and confocal microscopy (CM) provides trauma surgeons with the ability to promptly view comprehensive and detailed images of an injury. Of the aforementioned imaging modalities, only AS-OCT and CM can be directly controlled and localized by the ophthalmologist allowing for real time in vivo examination, directed high resolution observation of the anterior segment, and sequential imaging, thereby facilitating the diagnosis and management of the ocular injury.…”

Section: Discussionmentioning

confidence: 99%

Anterior Segment Imaging in Combat Ocular Trauma

Ryan

Sia

Colyer

et al. 2013

Journal of Ophthalmology

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Purpose. To evaluate the use of ocular imaging to enhance management and diagnosis of war-related anterior segment ocular injuries. Methods. This study was a prospective observational case series from an ongoing IRB-approved combat ocular trauma tracking study. Subjects with anterior segment ocular injury were imaged, when possible, using anterior segment optical coherence tomography (AS-OCT), confocal microscopy (CM), and slit lamp biomicroscopy. Results. Images captured from participants with combat ocular trauma on different systems provided comprehensive and alternate views of anterior segment injury to investigators. Conclusion. In combat-related trauma of the anterior segment, adjunct image acquisition enhances slit lamp examination and enables real time In vivo observation of the cornea facilitating injury characterization, progression, and management.

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“…[689] Most recently, Lavinsky et al ., evaluated six eyes of six consecutive patients within 30 days of sustaining blunt ocular trauma secondary to rock ( n = 3), piece of wood ( n = 1), and motor vehicle accident ( n = 2). [5] In this study, three patients presented with RPE epitheliopathy and three with subretinal hemorrhage with choroidal rupture.…”

Section: Discussionmentioning

confidence: 99%

“…[56] In this report, we demonstrate the use of non-mydriatic fundus imaging with digital filters, FAF, OCT, and microperimetry in illustrating the full structural and functional sequelae of posterior segment manifestations of blunt ocular trauma secondary to projectile objects.…”

Section: Introductionmentioning

confidence: 99%

Complimentary imaging technologies in blunt ocular trauma

et al. 2013

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We describe complimentary imaging technologies in traumatic chorioretinal injury. Color and fundus autofluorescence (FAF) images were obtained with a non-mydriatic retinal camera. Optical coherence tomography (OCT) helped obtain detailed images of retinal structure. Microperimetry was used to evaluate the visual function. A 40-year-old man sustained blunt ocular trauma with a stone. Color fundus image showed a large chorioretinal scar in the macula. Software filters allowed detailed illustration of extensive macular fibrosis. A 58-year-old man presented with blunt force trauma with a tennis ball. Color fundus imaging showed a crescentric area of macular choroidal rupture with fibrosis. FAF imaging delineated an area of hypofluorescence greater on fundus imaging. OCT showed chorioretinal atrophy in the macula. Microperimetry delineated an absolute scotoma with no response to maximal stimuli. Fundus imaging with digital filters and FAF illustrated the full extent of chorioretinal injury, while OCT and microperimetry corroborated the structure and function correlations.

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Anterior segment optical coherence tomography in eye injuries

Abstract: AS OCT is a very valuable tool in ophthalmic departments dealing with ocular trauma, for early diagnosis and for monitoring treatment progress.

Cited by 55 publications

References 17 publications

Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests

Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests

Anterior Segment Imaging in Combat Ocular Trauma

Complimentary imaging technologies in blunt ocular trauma

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