Anterior Capsular Plaque in Congenital Cataract: Occurrence, Morphology, Immunofluorescence, and Ultrastructure

Johar, Kaid; Vasavada, Abhay R.; Tatsumi, Kouko; Dholakia, Sheena A.; Nihalani, Bharti R.; Rao, Sarraju S. Lakshmana

doi:10.1167/iovs.07-0312

Cited by 17 publications

(10 citation statements)

References 31 publications

(35 reference statements)

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“…This substantiates previous immunohistologic studies identifying collagen-I-rich cells in congenital cataracts. 49 In general, collagen-I is involved in TGFb-related diseases such as idiopathic lung fibrosis, systemic sclerosis, and certain types of cancer. 50,51 Overexpression of COL1A2 in pediatric lens cells with its fibrotic and migrating patterns may contribute to high incidence of PCO and VAO in children.…”

Section: Effect Of Tgfb On Plecmentioning

confidence: 99%

Epithelial-Mesenchymal Transdifferentiation in Pediatric Lens Epithelial Cells

Wernecke

Keckeis

Reichhart

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Posterior capsule opacification (PCO) is a complication after cataract surgery, particularly in children. Epithelial-mesenchymal transition (EMT) of lens epithelial cells, mediated by transforming growth factor beta (TGFb), contributes to PCO. However, its pathogenesis in children is poorly understood. We correlated cell growth in culture with patient characteristics, studied gene expression of pediatric lens epithelial cells (pLEC), and examined the effects of TGFb-2 on these cells in vitro. METHODS. Clinical characteristics of children with cataracts correlated with growth behavior of pLEC in vitro. mRNA expression of epithelial (aB-crystallin, connexin-43) and mesenchymal (a V-integrin, a-smooth muscle actin, collagen-Ia2, fibronectin-1) markers was quantified in pLEC and in cell line HLE-B3 in the presence and absence of TGFb-2. RESULTS. Fifty-four anterior lens capsules from 40 children aged 1 to 180 months were obtained. Cell outgrowth occurred in 44% of the capsules from patients 12 months and in 33% of capsules from children aged 13 to 60 months, but in only 6% of capsules from children over 60 months. TGFb-2 significantly upregulated expression of aB-crystallin (HLE-B3), a V-integrin (HLE-B3), collagen-Ia2, and fibronectin-1 (in pLEC and HLE-B3 cells). CONCLUSIONS. Patient characteristics correlated with growth behavior of pLEC in vitro, paralleling a higher clinical incidence of PCO in younger children. Gene expression profiles of pLEC and HLE-B3 suggest that upregulation of a V-integrin, collagen-Ia2, and fibronectin-1 are involved in EMT.

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Section: Effect Of Tgfb On Plecmentioning

confidence: 99%

Epithelial-Mesenchymal Transdifferentiation in Pediatric Lens Epithelial Cells

Wernecke

Keckeis

Reichhart

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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“…They are thought to occur secondary to the differentiation of epithelial mesenchymal cells into myofibroblasts that secrete extracellular matrix. 4 The plaque seen here was supracapsular, adherent to its external surface. Although careful removal of the plaque avoids unnecessary lensectomy, an iatrogenic secondary cataract may occur.…”

Section: Commentmentioning

confidence: 66%

TORCH testing in non-familial paediatric cataract

Loone

Joyce

Coyle

2016

Eye

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“…This abnormal differentiation of LECs is involved in the pathogenesis of plaque in the intact lens and in the development of PCO and ACO after the removal of cataract. 2,4,13,14 We believe that the observed cells in this study could have been detached from any of the three zones of the lens epithelium. In our previous experimental study on human cadaver eyes, we observed that hydrodissection followed by rotation of the nucleus effectively scraped the LECs from the lens capsule.…”

Section: Discussionmentioning

confidence: 76%

Confirmation of the presence of lens epithelial cells in the anterior chamber after phacoemulsification

Vasavada

Johar

Praveen

et al. 2008

Eye

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Aims To detect the presence of lens epithelial cells in the anterior chamber of the eye at the end of phacoemulsification. Methods A prospective observational study was carried out on 50 patients undergoing phacoemulsification. Fluid from the anterior chamber was collected from these patients at the end of phacoemulsification. Thirty samples were processed for detection of viability using calcein AM-propidium iodide. Remaining samples were processed for immunofluorescence detection of aA-crystallin and vimentin. The nonparametric Mann-Whitney U test was applied.Results The presence of lens epithelial cells was confirmed in 27 of the first 30 samples. The total number of cells observed in these 27 samples were 64.70 ± 58.49. Within these 27 samples, 35.5% were live cells and 64.5% were dead. The cells were present as single cell or in groups. Twenty three percentage samples were also positive for nucleated lens fibres. In the remaining 20 samples, 89% cells were confirmed to be lens epithelial cells. Conclusions We show for the first time, the presence of cells in the fluid of the anterior chamber at the end of phacoemulsification. The cells were positive for aA-crystallin and vimentin, thereby suggesting that they were lens epithelial cells.

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Anterior Capsular Plaque in Congenital Cataract: Occurrence, Morphology, Immunofluorescence, and Ultrastructure

Abstract: The occurrence of ACP in pediatric eyes undergoing cataract surgery for congenital cataract was 11.5%. ACP was more associated with mature cataract. Epithelial mesenchymal transdifferentiation of lens epithelial cells may be involved in the development of congenital ACP.

Cited by 17 publications

References 31 publications

Epithelial-Mesenchymal Transdifferentiation in Pediatric Lens Epithelial Cells

Epithelial-Mesenchymal Transdifferentiation in Pediatric Lens Epithelial Cells

TORCH testing in non-familial paediatric cataract

Confirmation of the presence of lens epithelial cells in the anterior chamber after phacoemulsification

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