Antennal transcriptome of
            <i>Manduca sexta</i>

Große-Wilde, Ewald; Kuebler, Linda S.; Bucks, Sascha; Vogel, Heiko; Wicher, Dieter; Hansson, Bill S.

doi:10.1073/pnas.1017963108

Cited by 235 publications

(289 citation statements)

References 87 publications

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“…Thus regions of activity were set and analyzed separately for each individual. Coordinates were established to mark the center of activity regions and to superimpose square areas of interest in which the mean activity was calculated over 11 ϫ 11 pixels covering an area of 44 ϫ 44 m. The areas of interest corresponded to 50% of an averaged sized AL glomerulus in Manduca (Grosse-Wilde et al, 2011). We assigned individual coordinates to 5-10 activity regions per animal.…”

Section: Optophysiologymentioning

confidence: 99%

Antennal Lobe Processing Correlates to Moth Olfactory Behavior

Kuebler

Schubert²,

Kárpáti

et al. 2012

J. Neurosci.

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Animals typically perceive their olfactory environment as a complex blend of natural odor cues. In insects, the initial processing of odors occurs in the antennal lobe (AL). Afferent peripheral input from olfactory sensory neurons (OSNs) is modified via mostly inhibitory local interneurons (LNs) and transferred by projection neurons (PNs) to higher brain centers. Here we performed optophysiological studies in the AL of the moth, Manduca sexta, and recorded odor-evoked calcium changes in response to antennal stimulation with five monomolecular host volatiles and their artificial mixture. In a double staining approach, we simultaneously measured OSN network input in concert with PN output across the glomerular array. By comparing odor-evoked activity patterns and response intensities between the two processing levels, we show that host mixtures could generally be predicted from the linear summation of their components at the input of the AL, but output neurons established a unique, nonlinear spatial pattern separate from individual component identities. We then assessed whether particularly high levels of signal modulation correspond to behavioral relevance. One of our mixture components, phenyl acetaldehyde, evoked significant levels of nonlinear input-output modulation in observed spatiotemporal activation patterns that were unique from the other individual odorants tested. This compound also accelerated behavioral activity in subsequent wind tunnel tests, whereas another compound that did not exhibit high levels of modulation also did not affect behavior. These results suggest that the high degree of input-output modulation exhibited by the AL for specific odors can correlate to behavioral output.

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Section: Optophysiologymentioning

confidence: 99%

Antennal Lobe Processing Correlates to Moth Olfactory Behavior

Kuebler

Schubert²,

Kárpáti

et al. 2012

J. Neurosci.

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“…Since 1959, when the first long-range sex pheromone was chemically characterized by Butenandt et al [12], long-distance pheromone communication has been subjected to substantial research, capturing the attention of ecologists, evolutionary biologists, biochemists, neurophysiologists and molecular biologists (e.g. [13][14][15]). Long-range sex pheromones, which are usually composed of few components, have been assumed to serve solely as species recognition signals and therefore, to be subjected to a high degree of stabilizing selection (e.g.…”

Section: Introductionmentioning

confidence: 99%

Beyond species recognition: somatic state affects long-distance sex pheromone communication

Chemnitz¹,

Jentschke²,

Ayasse³

et al. 2015

Proc. R. Soc. B.

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Long-range sex pheromones have been subjected to substantial research with a particular focus on their biosynthesis, peripheral perception, central processing and the resulting orientation behaviour of perceivers. Fundamental to the research on sex attractants was the assumption that they primarily coordinate species recognition. However, especially when they are produced by the less limiting sex (usually males), the evolution of heightened condition dependence might be expected and long-range sex pheromones might, therefore, also inform about a signaller's quality. Here we provide, to our knowledge, the first comprehensive study of the role of a male's long-range pheromone in mate choice that combines chemical analyses, video observations and field experiments with a multifactorial manipulation of males' condition. We show that the emission of the long-distance sex pheromone of the burying beetle, Nicrophorus vespilloides is highly condition-dependent and reliably reflects nutritional state, age, body size and parasite load-key components of an individual's somatic state. Both, the quantity and ratio of the pheromone components were affected but the time invested in pheromone emission was largely unaffected by a male's condition. Moreover, the variation in pheromone emission caused by the variation in condition had a strong effect on the attractiveness of males in the field, with males in better nutritional condition, of older age, larger body size and bearing less parasites being more attractive. That a single pheromone is influenced by so many aspects of the somatic state and causes such variation in a male's attractiveness under field conditions was hitherto unknown and highlights the need to integrate indicator models of sexual selection into pheromone research.

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“…Antennal transcriptomes are available for insect species belonging to several orders, including Diptera, Coleoptera, and Lepidoptera (Grosse-Wilde et al, 2011;Andersson et al, 2013;Rinker et al, 2013;Leitch et al, 2015;Zhang et al, 2015). Within the Lepidoptera, the transcriptomes of model species such as B. mori, D. plexippus, H. melpomene, H. virescens, and M. sexta have been thoroughly investigated (Krieger et al, 2003(Krieger et al, , 2004Nakagawa et al, 2005;Wanner et al, 2007;Wanner and Robertson, 2008;Tanaka et al, 2009;Briscoe et al, 2013;Koenig et al, 2015;van Schooten et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Protein sequences conceptually translated from the assembled transcripts were aligned with homologs from Bombyx mori, Danaus plexippus, Heliconius melpomene, and Manduca sexta (Wanner and Robertson, 2008;Grosse-Wilde et al, 2011;Heliconius-Genome-Consortium, 2012;Briscoe et al, 2013;Koenig et al, 2015). In the case of GRs and ORs, sequences from the waterflea Daphnia pulex were also included as an outgroup (Peñalva-Arana et al, 2009).…”

Section: Alignments and Phylogenetic Treesmentioning

confidence: 99%

“…Annotation was performed using Blast2GO 3 (Conesa et al, 2005;Götz et al, 2008). Additionally, assembled transcripts belonging to target chemosensory families (OR, OBP, IR, GR, and CSP) were identified by comparison against custom, manually curated databases created using the available literature on other Lepidopteran species (Wanner and Robertson, 2008;Grosse-Wilde et al, 2011;Heliconius-Genome-Consortium, 2012;Briscoe et al, 2013;Koenig et al, 2015).…”

Section: Sequencing Assembly and Annotationmentioning

confidence: 99%

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Comparing the Expression of Olfaction-Related Genes in Gypsy Moth (Lymantria dispar) Adult Females and Larvae from One Flightless and Two Flight-Capable Populations

et al. 2017

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In insects, flight and sophisticated olfactory systems go hand in hand and are essential to survival and evolutionary success. Females of many Lepidopteran species have secondarily lost their flight ability, which may lead to changes in the olfactory capabilities of both larval and adult stages. The gypsy moth, Lymantria dispar, an important forest pest worldwide, is currently undergoing a diversification process with three recognized subspecies: the Asian gypsy moth (AGM), Lymantria dispar asiatica; the Japanese gypsy moth (JGM), Lymantria dispar japonica; and the European gypsy moth (EGM), Lymantria dispar dispar. Females of EGM populations from North America have lost their flight capacity whereas the JGM and AGM females are flight capable, making this an ideal system to investigate the relationship between flight and olfaction. We used next-generation sequencing to obtain female antennal and larval head capsule transcriptomes in order to (i) investigate the differences in expression of olfaction-related genes among populations; (ii) identify the most similar protein sequences reported for other organisms through a BLAST search, and (iii) establish the phylogenetic relationships of these sequences with respect to other insect species. Using this approach, we identified 115 putative chemosensory genes belonging to five families of olfaction-related genes. A principal component analysis (PCA) revealed that the gene-expression patterns of female antennal transcriptomes from different subspecies were more similar to one another than to the larval head capsules of their respective subspecies supporting strong chemosensory differences between the two developmental stages. An analysis of the shared and exclusively expressed genes for three populations shows no evidence that loss of flight affects the number or type of genes being expressed. These results indicate either (a) that loss of flight does not impact the olfactory gene repertoire or (b) that the secondary loss of flight in American EGM populations may be too recent to have caused major changes in the genes being expressed. However, we found higher expression values for Clavijo McCormick et al.Gypsy Moth Olfactory Gene Expression most olfaction-related genes in EGM females, suggesting that differences in transcription rates could be an adaptation of flightless females to their chemical environment. Differences in olfactory genes and their expression in the larvae appear to be unrelated to the flight ability of adult females and are likely adaptations to different ecological pressures.

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Antennal transcriptome of Manduca sexta

Cited by 235 publications

References 87 publications

Antennal Lobe Processing Correlates to Moth Olfactory Behavior

Antennal Lobe Processing Correlates to Moth Olfactory Behavior

Beyond species recognition: somatic state affects long-distance sex pheromone communication

Comparing the Expression of Olfaction-Related Genes in Gypsy Moth (Lymantria dispar) Adult Females and Larvae from One Flightless and Two Flight-Capable Populations

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