Antennal transcriptome analysis and expression profiles of putative chemosensory soluble proteins in Histia rhodope Cramer (Lepidoptera: Zygaenidae)

Yang, Haibo; Dong, Jun-Feng; Sun, Ya-Lan; Hu, Zhenjie; Lv, Qi-Hui; DingXu, Li

doi:10.1016/j.cbd.2020.100654

Cited by 14 publications

(17 citation statements)

References 54 publications

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“…We identified some chemosensory soluble proteins, such as odorant binding proteins (OBPs), chemosensory proteins (CSPs) and Niemann-Pick type C2 proteins (NPC2s) in this diurnal moth. We found that H. rhodope might also have lost a PBP gene (Yang et al, 2020). Therefore, we hypothesize that H. rhodope could also have lost chemoreceptor genes associated with moth pheromone detection.…”

Section: Introductionmentioning

confidence: 80%

“…The female and male antennae yielded at least 21.62 and 21.39 million clean reads per sample, respectively. All clean reads from male and female samples were combined into an assembly that generated 50,218 unigenes with a mean length of 1,031.08 bp and an N50 length of 2,247 bp (Yang et al, 2020). A tBLASTn analysis was performed using available ORs, IRs, GRs and SNMPs protein sequences from lepidopteran species as 'queries' to identify candidate unigenes in H. rhodope.…”

Section: Identification Of Putative Chemosensory Receptor Proteinsmentioning

confidence: 99%

“…qRT-PCR primers (Table S1) were designed with Primer Premier 5 (Premier Biosoft International, CA, USA) and synthesized by Sangon Biotech Co., Ltd (Shanghai, China). The glyceraldehyde-phosphate dehydrogenase (GAPDH) gene was identified from the H. rhodope antennal transcriptome and used as the internal reference (Yang et al, 2020). The qRT-PCR was conducted on a StepOne Plus Real-time PCR System (Applied Biosystems, Foster City, CA, USA) using SYBR Premix ExTaq II (Tli RNaseH Plus) (Takara, Dalian, China).…”

Section: Expression Level Analysismentioning

confidence: 99%

“…In the present study, we identified candidate chemoreceptor genes based on our previous antennal transcriptomic data of H. rhodope (Yang et al, 2020). The phylogenetic relationships between the ORs, IRs, GRs and SNMPs of H. rhodope and other insect species were further investigated.…”

Section: Introductionmentioning

confidence: 99%

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Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Yang¹,

Dong²,

Sun³

et al. 2020

PeerJ

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Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), nine ionotropic receptors (IRs), eight gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR co-receptor (ORco), ionotropic receptors co-receptor, CO2 receptors, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, six ORs, five IRs and two GRs displayed female-biased expression, while two ORs displayed male-biased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

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Section: Introductionmentioning

confidence: 80%

Section: Identification Of Putative Chemosensory Receptor Proteinsmentioning

confidence: 99%

Section: Expression Level Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Yang¹,

Dong²,

Sun³

et al. 2020

PeerJ

Self Cite

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show abstract

“…qRT-PCR primers ( Supplementary Table S1) were designed with Primer Premier 5 (Premier Biosoft International, CA, USA) and synthesized by Sangon Biotech Co., Ltd (Shanghai, China). The glyceraldehyde-phosphate dehydrogenase (GAPDH) gene was identified from the H. rhodope antennal transcriptome and used as the internal reference (Yang et al, 2020). The qRT-PCR was conducted on a StepOne Plus Real-time PCR System (Applied Biosystems, Foster City, CA, USA) using SYBR Premix ExTaq II (Tli RNaseH Plus) (Takara, Dalian, China).…”

Section: Expression Level Analysismentioning

confidence: 99%

Peer Review #1 of "Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae) (v0.1)"

Montagné¹

2020

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Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), 9 ionotropic receptor s (IRs), 8 gustatory receptors (GRs) and 2 sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR coreceptor (ORco), ionotropic receptor co-receptor, CO 2 receptor, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, 6 ORs, 5 IRs and 2 GRs displayed female-biased expression, while 2 ORs displayed malebiased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

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Sexual communication in diurnal moths: behaviors and mechanisms

Chen

Wang

et al. 2020

Int J Trop Insect Sci

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Antennal transcriptome analysis and expression profiles of putative chemosensory soluble proteins in Histia rhodope Cramer (Lepidoptera: Zygaenidae)

Cited by 14 publications

References 54 publications

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Peer Review #1 of "Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae) (v0.1)"

Sexual communication in diurnal moths: behaviors and mechanisms

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