Antennal transcriptome analysis and expression profiles of odorant binding proteins in Eogystia hippophaecolus (Lepidoptera: Cossidae)

Abstract

BackgroundEogystia hippophaecolus (Hua et al.) (Lepidoptera: Cossidae) is the major threat to seabuckthorn plantations in China. Specific and highly efficient artificial sex pheromone traps was developed and used to control it. However, the molecular basis for the pheromone recognition is not known. So we established the antennal transcriptome of E. hippophaecolus and characterized the expression profiles of odorant binding proteins. These results establish and improve the basis knowledge of the olfactory receptive system, furthermore provide a theoretical basis for the development of new pest control method.ResultsWe identified 29 transcripts encoding putative odorant-binding proteins (OBPs), 18 putative chemosensory proteins (CSPs), 63 odorant receptors (ORs), 13 gustatory receptors (GRs), 12 ionotropic receptors (IRs), and two sensory neuron membrane proteins (SNMPs). Based on phylogenetic analysis, we found one Orco and three pheromone receptors of E. hippophaecolus and found that EhipGR13 detects sugar, EhipGR11 and EhipGR3 detect bitter. Nine OBPs expression profile indicated that most were the highest expression in antennae, consistent with functions of OBPs in binding and transporting odors during the antennal recognition process. OBP6 was external expressed in male genital-biased in, and this locus may be responsible for pheromone binding and recognition as well as mating. OBP1 was the highest and biased expressed in the foot and may function as identification of host plant volatiles.ConclusionsOne hundred thirty-seven chemosensory proteins were identified and the accurate functions and groups of part proteins were obtained by phylogenetic analysis. The most OBPs were antenna-biased expressed, which are involved in antennal recognition. However, few OBP was detected biased expression in the foot and external genitalia, and these loci may function in pheromone recognition, mating, and the recognition of plant volatiles.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3008-4) contains supplementary material, which is available to authorized users.

Differential transcriptome analysis reveals genes related to cold tolerance in seabuckthorn carpenter moth, Eogystia hippophaecolus

“…A t-test was performed for the qPCR data. β-Actin was used as the reference gene to normalize target gene expression [ 53 ].…”

Different Responses of Cytoplasmic and Endoplasmic Reticulum Hsp90 Genes from Eogystia hippophaecola (Lepidoptera: Cossidae) to Cold Stress

“…Specific primers (supplementary material 1) for qRT-PCR were designed based on the sequences of ORFs using Primer Premier 5.0 [45]. The E. hippophaecola actin gene was used as a reference [40,41,46] and we used Bestkeeper to evaluate the std value (<1) of actin gene under different temperatures which indicated that actin gene was stable [47]. The qRT-PCR was carried out on a Bio-Rad CFX96 PCR machine (Hercules, CA) in a reaction mixture of 12. in three biological replicates, and each sample was technically repeated three times.…”

Antennal transcriptome analysis and expression profiles of odorant binding proteins in two woodwasps, Sirex noctilio and S. nitobei (Hymenoptera: Siricidae)

“…OBPs are soluble proteins with low molecular weights and are mainly expressed in the lymph of antenna sensilla [28][29]. Some OBPs exhibit specific binding with hydrophobic odorant molecules and can deliver the molecules to membrane-binding receptors [30][31][32][33]. Typical OBPs have six conserved cysteine residues, which form three pairs of disulfide bridges [28][29][34][35].…”