Antenatal steroid and tracheal occlusion restore vascular endothelial growth factor receptors in congenital diaphragmatic hernia rat model

Schmidt, Augusto Frederico; Gonçalves, Frances Lilian Lanhellas; Nassr, Azize Cristina Capelli; Farmer, Diana L.; Sbragia, Lourenço

doi:10.1016/j.ajog.2010.04.022

Cited by 16 publications

(18 citation statements)

References 31 publications

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“…Antenatal steroids may have a vital role in the regulation of vascular endothelial growth factor receptors that are instrumental in pulmonary angiogenesis and lung growth through nitric oxide-dependent mechanisms. 19,20 Disruption of vascular endothelial growth factor signaling because of premature birth impairs lung growth and sets the stage for late PH. [19][20][21] Lack of antenatal steroids has also been associated with decreased likelihood of closure of the ductus arteriosus.…”

Section: Discussionmentioning

confidence: 99%

“…19,20 Disruption of vascular endothelial growth factor signaling because of premature birth impairs lung growth and sets the stage for late PH. [19][20][21] Lack of antenatal steroids has also been associated with decreased likelihood of closure of the ductus arteriosus. 22 Although not statistically significant, infants with PH in our study were also more likely to have a prolonged PDA.…”

Section: Discussionmentioning

confidence: 99%

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Echocardiographic detection of pulmonary hypertension in extremely low birth weight infants with bronchopulmonary dysplasia requiring prolonged positive pressure ventilation

et al. 2011

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Objective: The goal of this study was to delineate the epidemiology of echocardiographically diagnosed pulmonary hypertension (PH) in extremely low birth weight (ELBW) infants with bronchopulmonary dysplasia (BPD) requiring prolonged positive pressure ventilation (PPV), and to determine the independent relationship between PH and mortality in these patients. Result:We identified 216 patients (19%) with BPD requiring prolonged PPV among 1156 ELBW infants. Of these patients, 41% received echocardiography after 4 weeks of life, with 37% showing evidence of PH. Logistic regression analysis demonstrated that infants with BPD requiring prolonged PPV, with PH detectable by echocardiogram, were four times more likely to die (adjusted odds ratio): 4.6, 95% confidence interval: 1.3-16.5) when compared with infants with BPD requiring prolonged PPV without echocardiographic evidence of PH.Conclusion: Pulmonary hypertension appears to be an important, independent determinant of death in infants with BPD requiring prolonged PPV.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Echocardiographic detection of pulmonary hypertension in extremely low birth weight infants with bronchopulmonary dysplasia requiring prolonged positive pressure ventilation

et al. 2011

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“…It has already been seen that in utero applications of VEGF or by means postnatal intra-tracheal instillation stimulates the production of surfactant, protecting against hyaline membrane disease [40]. It may also occur that the VEGF and its receptors can retrieve their expression involving other treatments than the ventilation or just them singly, for example, Schmidt et al found that added to the procedure TO retrieves the expression of the VEGF pathway components [41].…”

Section: Discussionmentioning

confidence: 99%

Effect of corticosteroids and lung ventilation in the VEGF and NO pathways in congenital diaphragmatic hernia in rats

et al. 2014

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“…The CDH model induced by nitrofen in fetal rats has been widely used to investigate the pathogenesis of diaphragmatic defect and other associated malformations similarly to humans 8,10 .…”

Section: Discussionmentioning

confidence: 99%

“…Among teratogens that are capable of inducing the defect, the most extensively studied and characterized model is the one utilizing the herbicide nitrofen (2,4-dichloro-4'nitrodiphenyl ether), that can cause left side CDH in 42% of the rat litter 8 .…”

Section: Introductionmentioning

confidence: 99%

Effect of nitrofen in the final stages of development of the diaphragm muscle in rats

et al. 2013

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Paulo (FMRP-USP). Ribeirao Preto -SP, Brazil. Mentor, conception and design of study, manuscript writing and critical revision. ABSTRACT PURPOSE:To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N-and N+ groups had decreased BW and DA compared to EC and OO groups (p <0.001). HA was decreased on GD 18.5 compared to 21.5 (p <0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased. Key words: Hernia. Diaphragmatic. Diaphragm. Abnormalities, Drug-Induced. Myosins. Rats. RESUMO OBJETIVO:Avaliar a expressão da miosina na muscularização do diafragma na hérnia diafragmática congênita (CDH) experimental. MÉTODOS: Fetos de ratas foram divididos em quatro grupos: Controle Externo (EC), composto de ratas não manipuladas; Nitrofen, composto de ratas que receberam 100 mg de nitrofen (2,4-dicloro-4'nitrodifenil éter) diluído no azeite no dia de gestação (GD) 9.5, cujos fetos desenvolveram CDH (N+) ou não (N-) e Placebo óleo de oliva (OO), composto de ratas que ingeriram apenas óleo no mesmo GD. Os fetos foram coletados com 18,5, 19,5, 20,5 e 21,5 GD (termo = 22 dias). Foi obtido o peso corporal (BW) e tiradas fotografias da área do diafragma (DA), da hérnia (HA) e calculada a relação HA/DA no grupo N+. Amostras de diafragmas foram processadas histologicamente para coloração com H/E e imunohistoquímica. RESULTADOS: Os fetos dos grupos N-e N+ tiveram BW e DA diminuídos em relação aos grupos EC e OO (p<0.001). Só houve diferença na HA entre os GD 18.5 e 21.5 (p<0.001) e a relação HA/DA não mostrou diferença entre os grupos. A imunohistoquímica mostrou menor expressão de miosina nos grupos que receberam nitrofen. CONCLUSÃO: O modelo de CDH induzida por nitrofen contribui para entender a muscularização na formação do diafragma onde a expressão da miosina está diminuída.

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Antenatal steroid and tracheal occlusion restore vascular endothelial growth factor receptors in congenital diaphragmatic hernia rat model

Cited by 16 publications

References 31 publications

Echocardiographic detection of pulmonary hypertension in extremely low birth weight infants with bronchopulmonary dysplasia requiring prolonged positive pressure ventilation

Echocardiographic detection of pulmonary hypertension in extremely low birth weight infants with bronchopulmonary dysplasia requiring prolonged positive pressure ventilation

Effect of corticosteroids and lung ventilation in the VEGF and NO pathways in congenital diaphragmatic hernia in rats

Effect of nitrofen in the final stages of development of the diaphragm muscle in rats

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