Antarctic tundra soil metagenome as useful natural resources of cold-active lignocelluolytic enzymes

Oh, Han Na; Park, Doyoung; Seong, Hoon Je; Kim, Dockyu; Sul, Woo Jun

doi:10.1007/s12275-019-9217-1

Cited by 20 publications

(10 citation statements)

References 59 publications

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“…Metagenomic surveys have allowed us to extract and analyze meaningful information about lignocellulolytic microbial communities from different natural environments [ 70 , 71 ]. Recently, genome-resolved metagenomics has been used to characterize different polymer-degrading consortia [ 20 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation

Rodríguez

Díaz-García

Bunk

et al. 2022

ISME Communications

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The understanding and manipulation of microbial communities toward the conversion of lignocellulose and plastics are topics of interest in microbial ecology and biotechnology. In this study, the polymer-degrading capability of a minimal lignocellulolytic microbial consortium (MELMC) was explored by genome-resolved metagenomics. The MELMC was mostly composed (>90%) of three bacterial members (Pseudomonas protegens; Pristimantibacillus lignocellulolyticus gen. nov., sp. nov; and Ochrobactrum gambitense sp. nov) recognized by their high-quality metagenome-assembled genomes (MAGs). Functional annotation of these MAGs revealed that Pr. lignocellulolyticus could be involved in cellulose and xylan deconstruction, whereas Ps. protegens could catabolize lignin-derived chemical compounds. The capacity of the MELMC to transform synthetic plastics was assessed by two strategies: (i) annotation of MAGs against databases containing plastic-transforming enzymes; and (ii) predicting enzymatic activity based on chemical structural similarities between lignin- and plastics-derived chemical compounds, using Simplified Molecular-Input Line-Entry System and Tanimoto coefficients. Enzymes involved in the depolymerization of polyurethane and polybutylene adipate terephthalate were found to be encoded by Ps. protegens, which could catabolize phthalates and terephthalic acid. The axenic culture of Ps. protegens grew on polyhydroxyalkanoate (PHA) nanoparticles and might be a suitable species for the industrial production of PHAs in the context of lignin and plastic upcycling.

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Section: Discussionmentioning

confidence: 99%

Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation

Rodríguez

Díaz-García

Bunk

et al. 2022

ISME Communications

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“…(South Shetland Island) (South Shetland Island) [22,27,29,[48][49][50][51][52]. These Actinobacteria have been widely reported in most metagenomics studies, specifically in Antarctic Peninsula areas, including in the Korean Antarctic Research Station and Alexander Island [53][54][55]. There are also other bacteria (less common) that have been listed as hydrocarbon-degrading bacterial species isolated from Antarctica and summarised by Wong et al (2021): Arthrobacter spp.…”

Section: Screening Of Wco and Pco-degrading Antarctic Bacterial Consortiamentioning

confidence: 99%

The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria

Zahri

Zulkharnain

Gómez-Fuentes

et al. 2021

Life

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Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in grey water. Such pollutants can persist for long periods in cold environments. The native microbial community may play a role in their biodegradation. In this study, using mixed native Antarctic bacterial communities, several environmental factors influencing biodegradation of waste canola oil (WCO) and pure canola oil (PCO) were optimised using established one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The factors include salinity, pH, type of nitrogen and concentration, temperature, yeast extract and initial substrate concentration in OFAT and only the significant factors proceeded for the statistical optimisation through RSM. High concentration of substrate targeted for degradation activity through RSM compared to OFAT method. As for the result, all factors were significant in PBD, while only 4 factors were significant in biodegradation of PCO (pH, nitrogen concentration, yeast extract and initial substrate concentration). Using OFAT, the most effective microbial community examined was able to degrade 94.42% and 86.83% (from an initial concentration of 0.5% (v/v)) of WCO and PCO, respectively, within 7 days. Using RSM, 94.99% and 79.77% degradation of WCO and PCO was achieved in 6 days. The significant interaction for the RSM in biodegradation activity between temperature and WCO concentration in WCO media were exhibited. Meanwhile, in biodegradation of PCO the significant factors were between (1) pH and PCO concentration, (2) nitrogen concentration and yeast extract, (3) nitrogen concentration and PCO concentration. The models for the RSM were validated for both WCO and PCO media and it showed no significant difference between experimental and predicted values. The efficiency of canola oil biodegradation achieved in this study provides support for the development of practical strategies for efficient bioremediation in the Antarctic environment.

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“…Arctic and Antarctic glaciers, snow, lakes, soil and marine sediments had their microbial community characterized and cold-adapted enzyme producing organisms were identified. Oh et al [ 115 ] used metagenomics in Antarctic soils to search for microorganisms able to produce cold-adapted lignolytic and cellulolytic enzymes. The metagenomics analysis found that 162 (1.42%) of a total of 11,436 genes were annotated as active carbohydrate enzymes.…”

Section: Extremozymes and Multi-omic Toolsmentioning

confidence: 99%

Extremophile Microbial Communities and Enzymes for Bioenergetic Application Based on Multi-Omics Tools

Fongaro

Maia

Rogovski

et al. 2020

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: Genomic and proteomic advances in extremophile microorganism studies are increasingly demonstrating their ability to produce a variety of enzymes capable of converting biomass into bioenergy. Such microorganisms are found in environments with nutritional restrictions, anaerobic environments, high salinity, varying pH conditions and extreme natural environments such as hydrothermal vents, soda lakes, and Antarctic sediments. Because extremophile microorganisms and their enzymes are found in widely disparate locations, they generate new possibilities and opportunities to explore biotechnological prospecting, including biofuels (biogas, hydrogen and ethanol) with an aim toward using multi-omics tools that shed light on biotechnological breakthroughs.

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Antarctic tundra soil metagenome as useful natural resources of cold-active lignocelluolytic enzymes

Cited by 20 publications

References 59 publications

Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation

Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation

The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria

Extremophile Microbial Communities and Enzymes for Bioenergetic Application Based on Multi-Omics Tools

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