Antagonistic reactions of arginine and lysine against formaldehyde and their relation to cell proliferation, apoptosis, folate cycle and photosynthesis

Effect of formaldehyde on cell proliferation and death

“…All these data suggest a considerable effect of repeatedly applied low doses of HCHO on cell proliferation and cell death. The previously mentioned observations of our group, (Tyihák et al, 2001), i.e. the apoptosis‐inducing effect of higher doses and the antiapoptotic and cell proliferation stimulatory effect of low doses of HCHO, may contribute to the damaging effect of this compound on DNA itself and its repair mechanism.…”

“…It is noteworthy that in the 0.5‐mM HCHO‐treated cultures, the proportion of cells in S‐phase increased significantly compared with the controls. The tumour cells appeared to be more sensitive than the endothelial cells (Tyihák et al, 2001).…”

Investigation of the Role of the Calvin Cycle and C1 Metabolism during HCHO Metabolism in Gaseous HCHO-Treated Petunia under Light and Dark Conditions Using13C-NMR

“…and the contribution of C1 metabolism increased in gaseous H 13 CHO-treated petunia plants under dark conditions. HCHO is usually detected in vivo in the form of an adduct with endogenous nucleophiles in higher plants (Mason et al, 1986;Sardi and Tyihak, 1994;Trezl et al, 2003). Accordingly, adduction with endogenous nucleophiles is suggested to be an effective strategy for detoxifying HCHO in vivo.…”

C1 metabolism and the Calvin cycle function simultaneously and independently during HCHO metabolism and detoxification in Arabidopsis thaliana treated with HCHO solutions

“…In higher plants, HCHO can be generated by several metabolic processes (Hanson & Roje ) and exists in vivo as adducts with endogenous nucleophiles (Mason, Sanders & Gidley ; Sardi & Tyihak ; Trezl et al . ). The study performed by Kallen & Jencks () observed that the aqueous HCHO can be rapidly condensed with tetrahydrofolate (THF) without enzyme catalysis in a solution to produce 5,10‐methylene‐THF (5,10‐CH 2 ‐THF).…”

“…However, the mechanism of HCHO metabolism in plants has been investigated in only a few studies, thereby the HCHO metabolic pathways in plants are not yet clearly defined. In higher plants, HCHO can be generated by several metabolic processes (Hanson & Roje 2001) and exists in vivo as adducts with endogenous nucleophiles (Mason, Sanders & Gidley 1986;Sardi & Tyihak 1994;Trezl et al 2003). The study performed by Kallen & Jencks (1966) observed that the aqueous HCHO can be rapidly condensed with tetrahydrofolate (THF) without enzyme catalysis in a solution to produce 5,10-methylene-THF (5,10-CH2-THF).…”