Antagonistic potential of Trichoderma harzianum and Azadirachta indica against Fusarium oxysporum f. sp. capsici for the management of chilli wilt

Jamil, Arshi; Musheer, Nasreen; Ashraf, Shabbir

doi:10.1007/s41348-020-00383-1

Cited by 21 publications

(13 citation statements)

References 38 publications

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“…Species of the genus Trichoderma are of great interest for their benefits in agriculture and natural ecosystems. Trichoderma species have antagonistic activity against several soil-borne and aerial-borne plant pathogens, mainly fungi such as Fusarium oxysporum [31,[71][72][73][74][75] [97] and Pythium ultimum [24,98], among many others.…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Fungal Diseases by Trichoderma aggressivum f. europaeum and Its Compatibility with Fungicides

Sánchez-Montesinos

Santos

Moreno-Gavíra

et al. 2021

JoF

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Our purpose was to evaluate the ability of Trichoderma aggressivum f. europaeum as a biological control agent against diseases from fungal phytopathogens. Twelve isolates of T. aggressivum f. europaeum were obtained from several substrates used for Agaricus bisporus cultivation from farms in Castilla-La Mancha (Spain). Growth rates of the 12 isolates were determined, and their antagonistic activity was analysed in vitro against Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium solani f. cucurbitae, Pythium aphanidermatum, Rhizoctonia solani, and Mycosphaerella melonis, and all isolates had high growth rates. T. aggressivum f. europaeum showed high antagonistic activity for different phytopathogens, greater than 80%, except for P. aphanidermatum at approximately 65%. The most effective isolate, T. aggressivum f. europaeum TAET1, inhibited B. cinerea, S. sclerotiorum, and M. melonis growth by 100% in detached leaves assay and inhibited germination of S. sclerotiorum sclerotia. Disease incidence and severity in plant assays for pathosystems ranged from 22% for F. solani to 80% for M. melonis. This isolate reduced the incidence of Podosphaera xanthii in zucchini leaves by 66.78%. The high compatibility by this isolate with fungicides could allow its use in combination with different pest management strategies. Based on the results, T. aggressivum f. europaeum TAET1 should be considered for studies in commercial greenhouses as a biological control agent.

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Section: Discussionmentioning

confidence: 99%

Biological Control of Fungal Diseases by Trichoderma aggressivum f. europaeum and Its Compatibility with Fungicides

Sánchez-Montesinos

Santos

Moreno-Gavíra

et al. 2021

JoF

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“…At 4 and 7 days of incubation, the diameter of mycelial growth of Fusarium strain in each confrontation was measured and used to calculate the percentage of inhibition by using the following formula: I = ( C − T )/ C × 100, where I represents the percentage of inhibition; C represents the pathogen's radial growth (in millimetres) when it is acting alone (the control); and T represents the pathogen's radial growth (in millimetres) when T. asperellum is present. The mycoparasitic activity, in particular, the invasion of Fusarium strain by T. asperellum , was evaluated from the point of contact between the two strains 7 days after incubation according to Jamil 35 …”

Section: Methodsmentioning

confidence: 99%

“…Trichoderma asperellum strains have long been studied for their characterization and antifungal efficacy against a variety of fungal plant diseases 31–37 . Studies on the chemical profile of T. asperellum strains, however, have only recently begun and have only focused on the major components and single extract fraction 38–41 .…”

Section: Introductionmentioning

confidence: 99%

Antagonistic activity of Trichoderma asperellum against Fusarium species, chemical profile and their efficacy for management of Fusarium‐root rot disease in dry bean

Elshahawy,

Marrez

2023

Pest Management Science

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BACKGROUNDDiseases caused by Fusarium pathogens lead to significant yield losses on many economically important crops. The purpose of this study was to evaluate the antagonistic capability and chemical profile of the bioagent Trichoderma asperellum against several Fusarium strains. The efficacy of this strain in reducing Fusarium‐root rot disease in dry bean was also examined.RESULTSThe T. asperellum strain was identified based on sequencing the ITS and tef1 gen regions of ribosomal DNA. Dual cultural assay demonstrated their antagonistic activity against the studied Fusarium strains due to the probable combination of competition, mycoparasitism and antibiosis. This strain was positive for cellulase, chitinase and protease activity. The crude extracts of T. asperellum significantly suppressed the growth of the tested Fusarium strains with inhibition zones values ranged from 7.3 to 19.7 mm and minimum inhibitory concentration (MIC) values ranged from 0.15 to 1.42 mg mL‐1. The GC‐MS analyses of cell free supernatant and mycelial biomass of T. asperellum showed the presence of 27 and 21 compounds, respectively. The main compounds responsible for the bioactivity were Butylated Hydroxytoluene, Hexadecanoic acid, 9‐Octadecenoic acid, Ergosterol and Hexadecanoic acid, ethyl ester. Trichoderma asperellum significantly increased plant emergence and reduced root rot caused by F. solani in dry bean grown under greenhouse and field trials. Further, plant biomass and dry bean yield were higher in T. asperellum‐treated plants than in control plants.CONCLUSIONTrichoderma asperellum was highly effective, through various mechanisms, against Fusarium strains especially F. solani which causes root rot in dry bean.This article is protected by copyright. All rights reserved.

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“…Moreover, the continuous use of synthetic fungicides can lead to the emergence of resistant pathogenic populations (Mehnaz et al, 2013;Fournier et al, 2020;Tleuova et al, 2020). Biological control using microbes that are antagonistic to pathogenic fungi is the right alternative because it does not have a negative impact on the environment (Lecomte et al, 2016;Jamil et al, 2020;Kalman et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies have reported that the application of microbes, both bacteria and fungus, is effective in suppressing the growth of Fusarium pathogens, including using Pseudomonas aeruginosa (Islam et al, 2018), Bacillus sp. (Jangir et al, 2018), Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 antagonist Foc Tropical Race 4 (Foc-TR4) (Wong et al, 2019), and Trichoderma harzianum (Jamil et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Antagonistic Activity and Characterization of Indigenous Soil Isolates of Bacteria and Fungi Against Onion Wilt Incited by Fusarium sp

Asmawati

Jumadi

2021

Preprint

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Tuber rot disease due to phytopathogen Fusarium oxysporum f. sp. cepae (Foc) infection is one of the main factors causing the decreasing amount of global shallot production. This study aims to find bacteria and fungi candidates which have Foc antagonistic activity through in vitro tests using dual culture techniques. A total of five bacterial isolates and three fungal isolates isolated from the rhizosphere of healthy onion plants showed the ability to inhibit Foc growth. B1 and B4 bacterial isolates had an average inhibitory capability of 65.93% and 72.27% respectively. Whereas C1 and C2 fungal isolates have the ability to inhibit the growth of Foc by as much as 74.82% and 67.76% respectively. The four tested microbial isolates were able to significantly inhibit Foc activity in vitro based on the ANOVA test, with values α = 0.05, and n = 3. Molecular analysis based on 16S-rRNA markers showed bacterial isolates B1 and B4 have an evolutionary relationship with B. subtilis. Whereas fungi C1 and C2 have evolutionary relationships with Aspergillus tubingensis and Trichoderma asperellum respectively, based on internal transcribed spacer (ITS) gene markers. The results of this study can be used to develop indigenous microbial consortiums as biological control agents for phytopathogenic fungi Fusarium oxysporum f. sp. cepae (Foc) on shallots.

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Antagonistic potential of Trichoderma harzianum and Azadirachta indica against Fusarium oxysporum f. sp. capsici for the management of chilli wilt

Cited by 21 publications

References 38 publications

Biological Control of Fungal Diseases by Trichoderma aggressivum f. europaeum and Its Compatibility with Fungicides

Biological Control of Fungal Diseases by Trichoderma aggressivum f. europaeum and Its Compatibility with Fungicides

Antagonistic activity of Trichoderma asperellum against Fusarium species, chemical profile and their efficacy for management of Fusarium‐root rot disease in dry bean

Antagonistic Activity and Characterization of Indigenous Soil Isolates of Bacteria and Fungi Against Onion Wilt Incited by Fusarium sp

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