Antagonistic effects of insulin and a negative growth regulator from ascites fluid on the growth of Ehrlich ascites carcinoma cells in vitro

Lehmann, Wolfram; Graetz, H; Schütt, M; Langen, P.

doi:10.1016/0014-4827(79)90370-7

Cited by 12 publications

(2 citation statements)

References 7 publications

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“…Mammary-derived growth inhibitor (MDGI), which suppresses carcinogenesis in transfected human breast cancer cells (Huynh et al, 1995) and inhibits the growth of Ehrlich ascitis carcinoma cells, was first detected in ascitic fluid (Lehmann et al, 1977(Lehmann et al, , 1979. MDGI belongs to a multigene family of fatty acid-binding proteins (FABP) known to bind long-chain fatty acids, retinoids and eicosanoids (Veerkamp et al, 1991).…”

Section: Mammary-derived Growth Inhibitor (Mdgi)mentioning

confidence: 98%

Cancer risk related to mammary gland structure and development

Russo

Yun

Silva

et al. 2001

Microsc. Res. Tech.

131

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Section: Mammary-derived Growth Inhibitor (Mdgi)mentioning

confidence: 98%

Cancer risk related to mammary gland structure and development

Russo

Yun

Silva

et al. 2001

Microsc. Res. Tech.

131

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“…The existence of a number of growth inhibitors has been postulated (1,2), and growth-inhibitory fractions have been obtained from various mammalian cells and tissues (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Nevertheless, there are doubts as to the significance of endogenous growth inhibitors because most of the inhibitor preparations have low specific activity, the inhibitors have been difficult to purify, and with some preparations the causes of growth inhibition have, at least in part, turned out to be trivial (15)(16)(17).…”

mentioning

confidence: 99%

Purification of kidney epithelial cell growth inhibitors.

Holley¹,

Bőhlen²,

Fava³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

154

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Two high molecular weight growth inhibitors have been isolated from the culture medium of BSC-1 cells, epithelial cells of African green monkey kidney. The purified kidney epithelial cell growth inhibitors, at ng/ml concentrations, reversibly arrest the growth of BSC-1 cells in the GI phase of the cell cycle. Their action is selective; they are most active on BSC-1 cells, are less active as inhibitors of the growth of rat lung and human breast epithelial cells, and do not inhibit the growth of 3T3 mouse embryo fibroblasts and human skin fibroblasts in culture. Their growth inhibitory action on BSC-1 cell cultures is counteracted by epidermal growth factor or calf serum.One of the areas of ignorance that remains in our broad understanding of the control of growth of mammalian cells is the subject of endogenous growth inhibitors. The existence of a number of growth inhibitors has been postulated (1, 2), and growth-inhibitory fractions have been obtained from various mammalian cells and tissues (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Nevertheless, there are doubts as to the significance of endogenous growth inhibitors because most of the inhibitor preparations have low specific activity, the inhibitors have been difficult to purify, and with some preparations the causes of growth inhibition have, at least in part, turned out to be trivial (15-17). There is no doubt that highly active growth inhibitors exist for mammalian cells, because interferon and corticotropin are extremely active as growth inhibitors in certain cell cultures (18,19), but the inhibitory activities of these two substances could be incidental to their other biological activities. Because of the potential significance of growth inhibitors and because purified inhibitors could be very useful in the manipulation of growth of mammalian cells, we have undertaken further studies in this area.Density-dependent regulation of growth of BSC-1 cells (African green monkey kidney epithelial cells) has been found to result in part from the accumulation of growth inhibitors in the culture medium (20). The present paper describes the isolation of high molecular weight growth inhibitors from the culture medium of these cells. The growth inhibitors have very high specific activities, approximately equal to the specific activities of interferon and corticotropin in their respective cell culture assays. The new growth inhibitors appear to be selective in the types of cells on which they act. MATERIALS AND METHODSCell Cultures. BSC-1 cell stock cultures were maintained as previously described (21) in Dulbecco's modified Eagle's medium (DME medium) (22) Preparation of Conditioned Medium. Dense BSC-1 cell cultures, used for preparation of conditioned medium, were grown in 15-cm Falcon tissue culture plates in DME medium with 1% calf serum. The medium was changed once a week until the cells reached a density of approximately 1.5 X 105 cells per cm2. The cultures were then maintained in DME medium with 0.1% calf serum. To prepare conditioned medium, the cultu...

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