Antagonistic Effect on Clostridium botulinum Type E by Organisms Resembling It

Kautter, D. A.; Harmon, S. M.; Lynt, R. K.; Lilly, T.

doi:10.1128/aem.14.4.616-622.1966

Cited by 41 publications

(34 citation statements)

References 10 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typing of strains of Clostridium botulinum is not based upon the serological and biochemical properties but upon the antigenic specificity of the toxins produced by the organisms. Toxin production, however, has proven to be an unstable character for classification because of nontoxigenic strains arising naturally from toxigenic strains with identical characteristics of cultural and biochemical properties as their parent strains [9,15]. On the other hand, the biochemical activities of the organisms separate them into proteolytic and nonproteolytic groups regardless of the toxin type and this classification shows a good agreement with the results of serological studies [22,27] and of DNA homology experiments [13].…”

supporting

confidence: 59%

Isolation of a Common Cell Wall Antigen from the Proteolytic Strains of Clostridium botulinum

Takumi

Kawata

1974

Japanese Journal of Microbiology

View full text Add to dashboard Cite

A protein‐rich antigen was extracted from the cell walls of Clostridium botulinum type A strain 190L by relatively mild treatments with detergents, tryptic digestion or cell wall autolysis. The cell wall antigen was relatively labile, and destroyed by acid or formamide hydrolysis. Immunodiffusion studies with the trypsin‐treated cell wall extracts from various strains of C. botulinum indicated that the antigenic cell wall polymers among the proteolytic strains of types A, B and F but not among the nonproteolytic strains of C. botulinum shared a common antigenic specificity.

show abstract

supporting

confidence: 59%

Isolation of a Common Cell Wall Antigen from the Proteolytic Strains of Clostridium botulinum

Takumi

Kawata

1974

Japanese Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Nontoxigenic organisms resembling Clostridium botulinum type E exist in environments where the food poisoning species may be present. Some of these clostridia are considered to be nontoxigenic forms of type E since the two cannot be distinguished in comparisons of morphology and biochemical activities (12) and antigens of the vegetative cells (13) and spores (16).…”

mentioning

confidence: 99%

Boticinogeny and Actions of the Bacteriocin

1971

View full text Add to dashboard Cite

The bacteriocin, boticin E, was produced by only a few strains of those clostridia which are nontoxigenic but otherwise identical to Clostridium botulinum type E. Boticin preparations from four different strains had identical spectra against indicator cultures. Experiments with bacterial lawns showed boticin to be sporostatic for all tested nonproteolytic C. botulinum (types B, E, and F) and nontoxigenic type E-related strains which included the producing strains as well as those different from type E in the fermentation of one to three carbohydrates. Boticin had no detectable effect on vegetative cells of boticinogenic strains but killed those of all other strains whose spores were sensitive. Cultures that were growing in an agar medium were more sensitive to the bacteriocin than those growing in broth. Vegetative cells of indicator strains adsorbed boticin, but cells of a boticin-resistant mutant did not. Boticin did not lyse suspensions of vegetative cells which had been killed previously by exposure to air but lysed actively growing protoplasts and Lforms of a strain whose normal vegetative cells are susceptible to lysis. Sporostasis resulted from inhibition of germination rather than of outgrowth. Proteolytic strains of C. botulinum (types A, B, and F) were resistant to boticin E. on August 1, 2020 by guest

show abstract

“…Therefore, it may be more likely that a small population of nontoxigenic organisms derived from the toxigenic organism was ingested by the patient with the toxigenic one. Since growth-inhibitory substances such as bacteriocin (1,4) were not found in the present study, it may be supposed that another kind of interaction, such as competition in attachment to the intestinal surface, plays an important role in the overgrowth of the nontoxigenic organisms during the convalescent phase. Accumulated information gathered from cases similar to the present case will lead to a better understanding of the role of nontoxigenic organisms in infant botulism.…”

mentioning

confidence: 55%