Antagonistic bacteria in the soil after cover crops cultivation

Abstract

The purpose of the studies was to establish the quantitative composition of bacteria Bacillus spp. and Pseudomonas spp. and their antagonistic effect towards soil-borne fungi after the cultivation of oats, spring vetch and tansy phacelia as intercrop cover plants. The total population of bacteria in 1 g dry weight of the soil from the experimental combination where mulch of oats was used was larger than in the combination with spring vetch or tansy phacelia. Totally, approximately three times as much Bacillus spp. was obtained from soil samples as compared to Pseudomonas spp. Tests showed that the most isolates of antagonistic bacteria out of the enumerated genera occurred in the soil after oats cultivation, and the least in the soil after the cultivation of tansy phacelia. Antagonistic Bacillus spp. and Pseudomonas spp. inhibited the growth and development of Fusarium oxysporum, Haematonectria haematococca and Thanatephorus cucumeris in the most effective way. The greatest total antagonistic effect of Bacillus spp. and Pseudomonas spp. towards Altenaria alternata, F. culmorum, F. oxysporum, H. haematococca, P. irregulare and T. cucumeris was found out after managing the mulch of oats. The smallest total antagonistic effect of bacteria was observed after managing the mulch of tansy phacelia.

Antagonistic activity of selected bacteria of the soil environment of carrot

“…The antagonistic effect of the studied bacteria towards the fungi species was the highest in the combination with oats (552 and 717, respectively) ( Table 1) and (Tables 1-3). A similar effect of the studied bacteria towards pathogenic fungi was shown by Patkowska and Konopiński (2014a) in the cultivation of scorzonera when cover crops were used. The ability of Bacillus spp.…”

“…and Bacillus spp. towards different species of pathogenic fungi was confirmed by a number of authors (Patkowska 2009, Alemu and Alemu 2013, Dalal and Kulkarni 2013, Saravanan et al 2013, Patkowska and Konopiński 2014a, Vanitha and Ramjegathesh 2014. Oats and vetch as cover crops used in the cultivation of scorzonera also increased the antagonistic effect of the bacteria towards soilborne fungi (Patkowska and Konopiński 2014a,b).…”

“…Microbiological analysis was made according to the methods described by Czaban et al (2007) and Patkowska and Konopiński (2014a). The soil was sampled from each experimental combination from four randomly chosen places.…”

“…(300 isolates from each genus) were used to establish their antagonistic effect towards fungi such as Altenaria dauci, A. radicina, Fusarium oxysporum, Thanatephorus cucumeris and Sclerotinia sclerotiorum (isolated from the infected carrot roots). While establishing the antagonistic effect of bacteria on pathogenic fungi, laboratory tests were conducted and the method and scale described by Martyniuk et al (1991), Patkowska and Konopiński (2014a) and Patkowska and Błażewicz-Woźniak (2014) were used. It took into consideration five degrees, i.e.…”

Antagonistic activity of selected bacteria occurring in the soil after root chicory cultivation

“…The most significant is the system of conserving cultivation (Borowy 2013, Patkowska and Konopiński 2013a, 2014. Mulching the soil with intercrop plants, left in the field for winter, protects the soil from erosion, excessive surface flow of the water, washing away of nutrients elements, and it increases the biological activity of the soil (Kołota and Adamczewska-Sowińska 2013).…”