Antagonistic Bacteria Against Schizophyllum Commune Fr. In Peninsular Malaysia

Dikin, Antarjo; Sijam, Kamaruzaman; Kadir, Jugah; Seman, Idris Abu

doi:10.11598/btb.2006.13.2.221

Cited by 10 publications

(4 citation statements)

References 9 publications

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“…Where, Ar = absorbance of reference (CAS solution and uninoculated broth), and As = absorbance of sample (CAS solution and cell-free supernatant of sample). The two strains were also screened for antifungal activities against Sclerotinia sclerotiorum (DSM 1946) using dual culture assay (Dikin et al, 2006), on potato dextrose agar (PDA) (Oxoid, Basingstoke, UK). The antagonistic activity was observed by measuring the size of the growth inhibition zone and the percentage of growth inhibition (PGI) was calculated using the formula: [(KR-R1)/KR] × 100 = % growth inhibition.…”

Section: In Vitro Assessment Of Pgp Traitsmentioning

confidence: 99%

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Guerrieri¹,

Fiorini²,

Fanfoni³

et al. 2021

Front. Plant Sci.

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Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination.

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Section: In Vitro Assessment Of Pgp Traitsmentioning

confidence: 99%

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Guerrieri¹,

Fiorini²,

Fanfoni³

et al. 2021

Front. Plant Sci.

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“…Veronaeopsis simplex is a dark septate endophytic fungus with the ability to suppress Fusarium wilt disease in Chinese cabbage ( Khastini et al, 2012 ). There was also a phytopathogen in Rhizo-Res, named Schizophyllum commune , that causes brown germ and seed rot of oil palm ( Dikin et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Microbial Communities and Functions in the Rhizosphere of Disease-Resistant and Susceptible Camellia spp.

Zhang

et al. 2021

Front. Microbiol.

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Oil tea (Camellia spp.) is endemic to the hilly regions in the subtropics. Camellia yuhsienensis is resistant to diseases such as anthracnose and root rot, while Camellia oleifera is a high-yield species but susceptible to these diseases. We hypothesize that differences in the rhizosphere microbial communities and functions will elucidate the resistance mechanisms of these species. We used high-throughput sequencing over four seasons to characterize the rhizosphere microbiome of C. oleifera (Rhizo-Sus) and C. yuhsienensis (Rhizo-Res) and of the bulk soil control (BulkS). In Rhizo-Res, bacterial richness and diversity (Shannon index) in autumn and winter were both higher than that in Rhizo-Sus. In Rhizo-Res, fungal richness in autumn and winter and diversity in summer, autumn, and winter were higher than that in Rhizo-Sus. The seasonal variations in bacterial community structure were different, while that of fungal community structure were similar between Rhizo-Res and Rhizo-Sus. Gram-positive, facultatively anaerobic, and stress-tolerant bacteria were the dominant groups in Rhizo-Sus, while Gram-negative bacteria were the dominant group in Rhizo-Res. The significant differences in bacterial and fungal functions between Rhizo-Sus and Rhizo-Res were as follows: (1) in Rhizo-Sus, there were three bacterial and four fungal groups with plant growth promoting potentials, such as Brevibacterium epidermidis and Oidiodendron maius, and one bacterium and three fungi with pathogenic potentials, such as Gryllotalpicola sp. and Cyphellophora sessilis; (2) in Rhizo-Res, there were also three bacteria and four fungal groups with plant-growth-promoting potentials (e.g., Acinetobacter lwoffii and Cenococcum geophilum) but only one phytopathogen (Schizophyllum commune). In summary, the rhizosphere microbiome of disease-resistant C. yuhsienensis is characterized by a higher richness and diversity of microbial communities, more symbiotic fungal communities, and fewer pathogens compared to the rhizosphere of high-yield but disease-susceptible C. oleifera.

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“…After three days, A. porri isolates seven days old, were inoculated into the center of the media, then incubated for four days at 28°C. Each treatment was repeated three times, then the percentage inhibition of the fungus radial growth was calculated using the formula according to Dikin et al (2006) with slight modifications as follows:…”

Section: Actinomycetes Antagonism Assay Against Alternaria Porrimentioning

confidence: 99%

Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri

WATI,

NAWANGSIH,

WAHYUDI

et al. 2023

Biodiversitas

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Abstract. Wati C, Nawangsih AA, Wahyudi AT, Wiyono S, Munif A. 2023. Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri. Biodiversitas 24: 5234-5242. Actinomycetes are microorganisms belonging to a group of Gram-positive bacteria, that have the potential to act as biological control agents against various plant pathogens. Phyllospheric Actinomycetes particularly, can inhibit growth of various microorganisms, such as the fungus Alternaria porri Ell. Cif., which causes purple blotch disease in the shallot plants. These microbes thrive on the surface of leaves, particularly in plants belonging to Liliaceae family. Environmental conditions have been proven to greatly affect their diversity. Present investigation aims to determine diversity of phyllospheric Actinomycetes from Liliaceae plants and their potential to inhibit growth of A. porri. The methods used were to investigate, characterize, and quantify isolates from Liliaceae plants. An antagonistic test was conducted on the isolates to examine the proportion of A. porri fungus growth inhibition. The results showed that plants species Allium fistulosum and A. tuberosum were the most common hosts for phyllospheric actinomycetes. Morphological differences were found in shape, color, elevation, edges, surface, and hyphae size, as well as the type of spore violation. The surface texture of colonies was smooth, rough, powdery, and opaque, usually appearing after 7-12 days of isolation. The isolates also inhibited growth of A. porri, causing the mycelium thins, and a clear zone of inhibition between pathogens and Actinomycetes forms. Isolates BCW9 caused the highest suppression of fungus growth 57.78%. This study is the first experiment to evaluate the diversity of phyllosphere Actinomycetes from Liliaceae plants, which are able to control purple blotch disease caused by A. porri in Indonesia. The phyllospheric Actinomycetes were isolated from the same habitat of A. porri. These investigation show the prospect of phyllospheric Actinomycetes as biocontrol agents against another fungal diseases on leaves of another plants.

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Antagonistic Bacteria Against Schizophyllum Commune Fr. In Peninsular Malaysia

Cited by 10 publications

References 9 publications

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Microbial Communities and Functions in the Rhizosphere of Disease-Resistant and Susceptible Camellia spp.

Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri

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