Antagonistic activity of olive endophytic bacteria and of Bacillus spp. strains against Xylella fastidiosa

Zicca, Stefania; Bellis, Palmira De; Masiello, Mario; Saponari, María; Saldarelli, P.; Boscia, D.; Sisto, Angelo

doi:10.1016/j.micres.2020.126467

Cited by 32 publications

(35 citation statements)

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“…The Elsinoaceae family is not well-studied but it is known to include plant pathogens [ 75 ]. This lack of consistency in cultivar- or resistance-associated Bacteria was also revealed in a companion paper [ 76 ] where endophytes, and among the others, members of the Methylobacterium and Curtobacterium genera previously indicated as potential biocontrol endophytes [ 31 , 32 ], were isolated from the same FS17 and Kalamata olives. All the isolated genera ( Methylobacterium , Sphingomonas , Curtobacterium , Novosphingobium , Frondihabitans , Agrococcus , and Micrococcus ) were identified in WMSS and the majority of them in 16S rRNA gene analysis, but none were found having in vitro antagonistic activity against Xylella [ 76 ].…”

Section: Discussionmentioning

confidence: 59%

“…This lack of consistency in cultivar- or resistance-associated Bacteria was also revealed in a companion paper [ 76 ] where endophytes, and among the others, members of the Methylobacterium and Curtobacterium genera previously indicated as potential biocontrol endophytes [ 31 , 32 ], were isolated from the same FS17 and Kalamata olives. All the isolated genera ( Methylobacterium , Sphingomonas , Curtobacterium , Novosphingobium , Frondihabitans , Agrococcus , and Micrococcus ) were identified in WMSS and the majority of them in 16S rRNA gene analysis, but none were found having in vitro antagonistic activity against Xylella [ 76 ]. In addition, the present work does not identify the association of microbial consortia with the host resistance, leaving open the possibility that other plant traits are responsible for controlling Xylella population size and its pathogenic effects.…”

Section: Discussionmentioning

confidence: 59%

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Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons

et al. 2020

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The dynamics of Xylella fastidiosa infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that Xylella tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome. However, this trend was mitigated in the resistant cultivar FS17, harboring lower population sizes and therefore lower Xylella average abundance ratio over total bacteria, and a higher α-diversity. Host cultivar had a negligible effect on the community composition and no clear associations of a single taxon or microbial consortia with the resistance cultivar were found with both sequencing approaches, suggesting that the mechanisms of resistance likely reside on factors that are independent of the microbiome structure. Overall, Proteobacteria, Actinobacteria, Firmicutes, and Bacteriodetes dominated the bacterial microbiome while Ascomycota and Basidiomycota those of Fungi.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 59%

Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons

et al. 2020

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“…Another explanation for this incremental trend is the previously introduced emerging awareness of a relationship between endophyte occurrence and pest and pathogen incidences in crops. In the case of olive tree, this concept has been particularly considered with reference to the outbreak of the quick decline syndrome incited by Xylella fastidiosa in southern Italy [45], although no significant associations of any identified endophytes with this bacterium were found in both a high and a low susceptible cultivar in a dedicated study [21]. In another study concerning relationships with another widespread bacterial pathogen, Pseudomonas savastanoi pv.…”

Section: Occurrence and Ecological Implications Of Endophytic Fungi Omentioning

confidence: 99%

Endophytic Fungi of Olive Tree

2020

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In addition to the general interest connected with investigations on biodiversity in natural contexts, more recently the scientific community has started considering occurrence of endophytic fungi in crops in the awareness of the fundamental role played by these microorganisms on plant growth and protection. Crops such as olive tree, whose management is more and more frequently based on the paradigm of sustainable agriculture, are particularly interested in the perspective of a possible applicative employment, considering that the multi-year crop cycle implies a likely higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with olive tree (Olea europaea) are revised in the present paper.

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“…Such type of beneficial bacteria present in the rhizospheric region of plant are known as Plant Growth Promoting Rhizobacteria (PGPR) 12 . In this way, PGPR can be a better alternative to enhance the plant health, increase yield, and control phytopathogens without causing any environmental contamination 13,14 . PGPR enhance the growth of crops either by the growth substances produced by them or by up taking the nutrients from the environment and supply to the plant.…”

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Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays

Kumar

Pahal

Gupta³

et al. 2020

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The effect of Plant Growth Promoting Rhizobacteria (Bacillus sp.) and silver nanoparticles on Zea mays was evaluated. The silver nanoparticles were synthesized from Tagetes erecta (Marigold) leaf and flower extracts, whereas PGPR isolated from spinach rhizosphere. The silver nanoparticles (AgNPs) were purified using ultra centrifugation and were characterized using UV–Vis spectroscopy at gradient wavelength and also by High Resolution Transmission Electron microscopy (HRTEM). The average particles size of AgNPs was recorded approximately 60 nm. Almost all potential isolates were able to produce Indole Acetic Acid (IAA), ammonia and Hydrogen cyanide (HCN), solubilized tricalcium phosphate and inhibited the growth of Macrophomina phaseolina in vitro but the isolate LPR2 was found the best among all. On the basis of 16S rRNA gene sequence, the isolate LPR2 was characterized as Bacillus cereus LPR2. The maize seeds bacterized with LPR2 and AgNPs individually showed a significant increase in germination (87.5%) followed by LPR2 + AgNPs (75%). But the maximum growth of root and shoot of maize plant was observed in seeds coated with LPR2 followed by AgNPs and a combination of both. Bacillus cereus LPR2 and silver nanoparticles enhanced the plant growth and LPR2 strongly inhibited the growth of deleterious fungal pathogen. Therefore, LPR2 and AgNPs could be utilized as bioinoculant and growth stimulator, respectively for maize.

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Antagonistic activity of olive endophytic bacteria and of Bacillus spp. strains against Xylella fastidiosa

Cited by 32 publications

References 33 publications

Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons

Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons

Endophytic Fungi of Olive Tree

Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays

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