Antagonistic activity of Lactobacillus casei strain Shirota against gastrointestinal Listeria monocytogenes infection in rats

Waard, Rick de; Garssen, Johan; Bokken, G.C.A.M.; Vos, J.G.

doi:10.1016/s0168-1605(01)00699-7

Cited by 88 publications

(66 citation statements)

References 42 publications

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“…With T. spiralis-infected Wistar and Brown Norway rats it was shown that oral L. casei supplementation enhanced both the antigen-specific DTH and serum IgG2b. In our hands, enhancement of antigen-specific DTH by peroral L. casei application has also been observed in a food-borne model of L. monocytogenes (7). Expression of antigen-specific DTH in rats and mice and of IgG2b responses in rats are reported to be manifestations of Th1-type cellmediated responses (15,33).…”

Section: Discussionsupporting

confidence: 59%

“…The immunostimulating activity of Lactobacillus casei Shirota was also observed in a rat model of food-borne Listeria monocytogenes infection. It was shown in orally Listeria-infected rats that ingestion of L. casei enhanced the DTH reaction (7). From these studies, and based on the described immune cell populations involved in DTH responses (29), it was asserted that orally supplemented L. casei could exert a direct effect on T-helper-1-cell immunity by modulating the amount and activity of antigen-specific T lymphocytes and/or could exert an indirect effect on T-lymphocyte activity through stimulation of other cell types, such as phagocytes.…”

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confidence: 99%

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Enhanced Immunological Memory Responses toListeria monocytogenesin Rodents, as Measured by Delayed-Type Hypersensitivity (DTH), Adoptive Transfer of DTH, and Protective Immunity, followingLactobacillus caseiShirota Ingestion

Waard¹,

Claassen²,

Bokken³

et al. 2003

Clin Vaccine Immunol

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We have investigated the effect of orally administered Lactobacillus casei Shirota (L. casei) on immunological memory, as measured by delayed-type hypersensitivity (DTH) and acquired cellular resistance (ACR). The studies were performed in animal models in which the animals were rendered immune by a primary Listeria monocytogenes infection. It was shown that orally administered viable L. casei, and not heat-killed L. casei, enhanced significantly the antigen-specific DTH at 24 and 48 h in Wistar rats, Brown Norway rats, and BALB/c mice in a time-and dose-dependent fashion. L. casei had to be administered at least 3 days prior to the DTH assay at a daily dose of 10 9 CFU in order to induce significant effects. Long-term administration of 10 9 CFU of viable L. casei resulted in enhanced ACR, as demonstrated by reduced L. monocytogenes counts in the spleen and liver and diminished serum alanine aminotransferase activity after reinfection. Enhancement of cellmediated immunological immune responses by L. casei was further established in an adoptive transfer study. Naïve recipient BALB/c mice, which were infused with nonadherent, immunized spleen cells from L. casei-fed donor BALB/c mice, showed significantly enhanced DTH responses at 24 and 48 h compared to recipient mice which received spleen cells from control donor mice. In conclusion, orally administered L. casei enhanced cell-mediated immunological memory responses. The effects relied on lactobacillus dose and viability as well as timing of supplementation and, further, appeared to be independent of host species or genetic background.Various cells of the innate and acquired immune system interact in a concerted fashion to constitute a sophisticated set of responses to protect the physiological integrity of the host against infectious agents and tumors. Inappropriate orchestrated immune responses lead to, e.g., autoimmune and allergic disease states as well as enhanced susceptibility to infections. Different compounds are known which have the capacity to enhance or decrease biological responses of the immune system in order to restore disturbed immune responses or to elevate resistance to infectious agents. These compounds can be classified as so-called immunomodulators or biologic response modifiers (23).Lactobacilli form a source of potential modulators of the immune system. It has been demonstrated that specific Lactobacillus strains can modulate host immunity, which positively correlates with enhanced resistance to various viral and bacterial infections (9,20,24). The immunomodulating effects are dependent on various factors, such as intrinsic adjuvanticity properties (21), dose (13, 31), viability (16, 20, 30), route and timing of administration of the specific Lactobacillus strains (i.e., oral versus parenteral), and the physiological state and genetic background of the host (20).Previous results from our group established that 10 9 orally administered viable Lactobacillus casei Shirota strain YIT9029 (L. casei) bacteria given daily enhanced Trichinella spi...

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Section: Discussionsupporting

confidence: 59%

mentioning

confidence: 99%

Enhanced Immunological Memory Responses toListeria monocytogenesin Rodents, as Measured by Delayed-Type Hypersensitivity (DTH), Adoptive Transfer of DTH, and Protective Immunity, followingLactobacillus caseiShirota Ingestion

Waard¹,

Claassen²,

Bokken³

et al. 2003

Clin Vaccine Immunol

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“…22,33 In a rat model of gastrointestinal Listeria monocytogenes infection, orally administered LcS augmented the delayed-type hypersensitivity response to Listeria-specific antigens and improved the severity of Listeria infection. 34 In a model of influenza virus infection, oral administration of LcS to infant mice enhanced IL-12 production by mediastinal lymph node cells and pulmonary NK cell activity and reduced the titer of influenza virus in the nasal cavity. 35 The combined results indicate that LcS can strongly induce IL-12 production through its specific cell wall structure and can augment host immune defense.…”

Section: Il-12 Production Induced By Lcs In Coexistence With Other Bamentioning

confidence: 99%

Flexible cytokine production by macrophages and T cells in response to probiotic bacteria: A possible mechanism by which probiotics exert multifunctional immune regulatory activities

Shida¹,

Nanno²,

Nagata³

2011

Gut Microbes

103

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“…These commensal strains have been shown to modulate the expression of genes involved in important physiologic functions such as postnatal intestinal maturation, cell growth, proliferation, nutrient absorption, mucosal barrier function, and angiogenesis [4][5][6] . Multiple laboratory studies have shown beneficial effects of Lactobacillus strains against single pathogenic bacterial strains in in vitro and in vivo systems [7,8] . During the last few years, there has been an exponential increase of clinical trial reports and reviews in the literature pertaining to the utility of probiotics in gastrointestinal and allergic diseases [9][10][11][12][13][14][15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology

Panigrahi

Braileanu

Chen

et al. 2007

WJG

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AIM:To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. METHODS:A 19 200 gene/expressed sequence tag gene chip was used to examine expression of genes after infection of Caco-2 cells with strains of normal flora E. coli , Lactobacillus plantarum , and a combination of the two. RESULTS:The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli . L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection, 27 genes were upregulated and 59 were down-regulated, with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. CONCLUSION:The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription, protein biosynthesis, metabolism, cell adhesion, ubiquitination, and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.

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Antagonistic activity of Lactobacillus casei strain Shirota against gastrointestinal Listeria monocytogenes infection in rats

Cited by 88 publications

References 42 publications

Enhanced Immunological Memory Responses toListeria monocytogenesin Rodents, as Measured by Delayed-Type Hypersensitivity (DTH), Adoptive Transfer of DTH, and Protective Immunity, followingLactobacillus caseiShirota Ingestion

Enhanced Immunological Memory Responses toListeria monocytogenesin Rodents, as Measured by Delayed-Type Hypersensitivity (DTH), Adoptive Transfer of DTH, and Protective Immunity, followingLactobacillus caseiShirota Ingestion

Flexible cytokine production by macrophages and T cells in response to probiotic bacteria: A possible mechanism by which probiotics exert multifunctional immune regulatory activities

Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology

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