Antagonistic Activity of Fungal Endophytes Isolated from Garcinia atroviridis against Colletotrichum gloeosporioides

Abstract

The extensive use of synthetic fungicides in controlling plant disease generates detrimental impacts on the environment and human health. In response to this problem, an alternative method was developed, known as biological control using antagonistic microorganisms. Since investigation on fungal endophytes of Garcinia atroviridis is still unclear, it was chosen for the study. The aim of the present work was to evaluate biocontrol potential of endophytic fungi against Colletotrichum gloeosporiodes, a phytopathogen that caused anthracnose disease. A total of 92 endophytic fungi were isolated from different tissue parts of Garcinia atroviridis including leaves, petioles, branches, and fruits. Results demonstrated that, most of endophytic fungal isolates showed some inhibitory action over the growth of C. gloeosporiodes during dual culture growth. Endophyte isolate F14 showed the highest antagonistic activity against Colletotrichum gloeosporiodes with 67.38% percentage inhibition radial growth (PIRG). However, 7 out of 92 isolates showed no inhibitory effect against Colletotrichum gloeosporiodes. In conclusion, endophytic fungi isolated from G. atroviridis indicate the potential as biocontrol agents. It is hoped that the finding of isolated endophytic fungi in this study with antagonistic activity against anthracnose pathogen may be used in biocontrol programmes of plant disease in the region.

Endophytic fungi: versatile partners for pest biocontrol, growth promotion, and climate change resilience in plants

“…Furthermore, Billar de Almeida et al ( 2010) also reported the possibility of using EFs for the management of grapevine trunk diseases, which are very important and widespread fungal diseases impacting grapevines. Several other reports on plantpathogen suppression following the treatment of crops with EFs are well documented (Hassanein et al, 2020;Zanudin et al, 2020;Aldinary et al, 2021;Mota et al, 2021;Table 2).…”

Isolasi dan karakterisasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” dan potensinya menghambat penyakit bercak ungu Alternaria porri (ELL) CIF

“…Lebih (Putri et al, 2022). Isolat endofit F14 yang diisolasi dari tanaman Garcinia atroviridis memiliki aktivitas antagonis tertinggi terhadap fitopatogen Colletotrichum gloeosporiodes penyebab penyakit antraknosa (Zanudin et al, 2020). Cendawan endofit Trichoderma harzianum dan Penicillium citrinum menghasilkan daya hambat 73,1% dan 60,04% terhadap patogen A. porri (Abdel-Hafez et al, 2015;Firdausi et al, 2021).…”

Fungal Endophytes and Their Role in Agricultural Plant Protection against Pests and Pathogens

“…Other studies use a different approach to determine the specific antifungal compounds that may control plant pathogens. In these studies, the fungal endophytic diversity of a host plant species is characterized, and endophyte cultures are selected for dual culture assays to assess antagonism against known pathogens of the host plant [ 44 , 61 , 62 , 63 , 64 ]. Antagonism against pathogens is primarily determined by the presence of inhibition zones between the endophytic and pathogenic fungi, or the ability of the endophytic fungi to overgrow the pathogenic fungi [ 61 , 62 , 65 , 66 , 67 ].…”

“…In these studies, the fungal endophytic diversity of a host plant species is characterized, and endophyte cultures are selected for dual culture assays to assess antagonism against known pathogens of the host plant [ 44 , 61 , 62 , 63 , 64 ]. Antagonism against pathogens is primarily determined by the presence of inhibition zones between the endophytic and pathogenic fungi, or the ability of the endophytic fungi to overgrow the pathogenic fungi [ 61 , 62 , 65 , 66 , 67 ]. Cultures showing anti-pathogen activities undergo compound extraction and analysis with liquid chromatography or gas spectrometry run in tandem with a mass spectrometry [ 59 , 60 , 68 ].…”

“…Cultures showing anti-pathogen activities undergo compound extraction and analysis with liquid chromatography or gas spectrometry run in tandem with a mass spectrometry [ 59 , 60 , 68 ]. The results of such studies aid in the identification of candidate endophyte species, which can be further investigated for their biocontrol potential, and consistently show that antagonists of pathogens are an inherent part of the plant microbiome [ 61 , 62 , 69 , 70 ]. The antifungal activities of compounds produced by some endophytes have been studied for their mode of effectiveness against several different pathogenic fungi and their ability to increase host plant fitness [ 44 ].…”