Antagonistic Activity of Fungal and Bacterial Bio-Inoculants Against Sclerotium Rolfsii

Abstract

The present studies were undertaken to investigate the effect of fungal and bacterial biocontrol agents against stem rot of groundnut. The result of the dual culture technique indicated that Trichoderma isolates inhibited the growth of S. rolfsii. Among the isolate T. viride (Tv1 ) produced maximum reduction of mycelial growth. This was followed by the isolates T. harziaum and T. virens which restricted the mycelial growth when compared to control. Among the isolates T. viride (Tv1 ) at a conc. of 10, 20, 30 and 40 per cent conc. showed an increase in the inhibition of the mycelia growth recording 22.15, 15.27, 8.75 and 0.00 mm respectively. The next best in antagonist was T. harzianum. Among the Pseudomonas fluorescens isolates, PfI3 produced maximum reduction of mycelial growth accounting for 74.97 per cent reduction over control. Also, a general increase in the conc. of the Antagonistic culture filtrate showed an increase in the inhibition of the mycelial growth of the test pathogen.

Negative impact of some fungicide applications on Trichoderma harzianum as biocontrol agent of shallot moler disease

“…Antibiosis mechanism by producing volatile components and non-volatile antibiotics [15] that obstruct colonization by antagonized microorganisms [23]. A large variety of volatile secondary metabolites could be produced by Trichoderma such as ethylene, hydrogen cyanide, aldehydes and ketones [25,26]. The potential of volatile and non-volatile antifungal compounds that are major players in the control of pathogens [27,28].…”