Antagonistic Activity of Bacteriocin-producing Lactobacillus Against Candida spp

Mohsin, Zainab Abdulridha; Ali, Wala’a Shawkat

doi:10.24996/ijs.2021.62.7.4

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…Research findings are consistent with those of Mohsin and Ali, who cultured swabs on (SDA), then identified all isolates macroscopically and microscopically following incubation (Mohsin and Ali, 2021) Habib and his colleagues., who used CHROM agar to identify different species of Candida causing vulvovaginal Candidiasis (Habib et al, 2016). Othman et al, who identifying species of Candida after culturing samples on Chromogenic agar Candida (Othman et al, 2018).…”

Section: (3)supporting

confidence: 86%

Isolation and Identification of Candida dubliniensis in Patient with Cervical-Vulvovaginal Candidiasis in Erbil City

Abdulla¹,

Ismael²

2022

RJS

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Vulvovaginal Candidiasis (VVC) is a fungal infection caused by Candida spp. of the genital mucosa that is still a health concern for women. This is a first-of-its-kind investigation on the isolation of Candida dubliniensis, from cervical-vulvovaginal patients in Erbil hospitals. The study was carried out for isolation and identification which performed by using phenotypic and molecular approaches. A total of one hundred samples of vaginal swabs (Copan transport swabs dry (in a cover)), were taken from patients with cervical vulvovaginal candidiasis in order to test for the presence of Candida species. According to macroscopic and microscopic examination, Candida spp. was discovered in 60 of the 100 samples (60%). On CHROM agar medium, eleven nonduplicated C. dubliniensis were identified depending on color of colony (dark green colonies-blue colony). Then the species were confirmed using API 20C assay. Finally, a multiplex PCR technique was performed depending on two types of primers, which are universal and specific for identifying C. dubliniensis and the results of the CHROM agar and API 20C assays were identical with molecular method.

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Section: (3)supporting

confidence: 86%

Isolation and Identification of Candida dubliniensis in Patient with Cervical-Vulvovaginal Candidiasis in Erbil City

Abdulla¹,

Ismael²

2022

RJS

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“…The PCR assay is a potent approach for identifying various genes, it is quicker, easier, and more accurate (Mohsin and Ali, 2021;Sheet, 2022).…”

Section: Molecular Identification Of C Albicansmentioning

confidence: 99%

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Mahmoud¹,

Yassein²

2023

AAVS

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The aims of this study are to identify mycotic agents, assess the prevalence of mycotic mastitis, and evaluate the virulence of C. albicans that was isolated from Mastitic goats. To investigate the prevalence of mycotic mastitis, related to goats in Baghdad, 166 lactating goats were examined and 332 milk samples were collected from ( January -July) 2022. 10 ml of milk were collected in sterile test tubes, an equal volume of milk and CMT reagent were gently shaken in a paddle and the reaction was noticed within (10-15) seconds. The clinical form of mastitis accounted 9.35%, while the subclinical form accounted 52.4%, with positive (+ve) California mastitis test (CMT) results which are high significant. All milk samples were cultured on Sabouraud dextrose agar (SDA) for (3-5) days at 37 ºC. According to the result, 46.34% of the isolates had mycotic mastitis, with 33 isolates (34.74%) belonging to mold and 62 isolates (65.26%) returning to yeasts. Candida albicans had the highest percentage (high significant) of yeast isolation of (67.74%) with 42 isolates which were detected by using conventional methods such as the germ tube test, urease production, Chlamydospore formation, and chromogenic medium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 100% of C. albicans isolates. The diagnosis was confirmed using 25 of the most virulent C. albicans isolates, by polymers chains reaction (PCR) to detect the KER1 gene revealed that all 25 samples were positive with the product size (658bp). Phospholipases B1 (PLB1) and the secretion of aspartyl proteinase 1 (SAP1) gene were used to detect C. albicans virulence with 100% and 80% accuracy, respectively, while Hyphal Wall Protein 1 (HWP1) gene was not detected. It is expected that these C. albicans isolates contained a significant proportion of virulence factors that were linked to pathogenicity and the severity of infection. There findings suggest that poor sanitation and a reduction in goat health and nutrition were to blame for the high morbidity incidence of mycotic mastitis in Iraqi goats.

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“…Inoculation of these isolates on CHROMagar was performed and C. albicans was found to be the most common yeast [24]. Mohsin and Ali (2021) cultured swabs on Sabouraud dextrose agar (SDA) and then identified all isolates macroscopically and microscopically following incubation [25]. Nadeem The results of the molecular identification by using universal and specific primers gene amplification were consistent with the phenotypic analysis which included C. albicans and C. lusitaniae.…”

Section: Resultsmentioning

confidence: 89%

The Efficacy of Antifungal Medications and Plant Extracts Against Candida albicans Isolated from Vulvovaginitis Women

Abdulla

Ismael²

2023

Iraqi Journal of Science

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Fungus Candida spp. causes vulvovaginal candidiasis (VVC) which is a fungal infection of the genital mucosa. This investigation was on the isolation of Candida albicans from cervical-vulvovaginal patients in Erbil hospitals, Kurdistan region, Iraq and its identification is done using phenotypic and molecular approaches. Then biofilm production was detected through phenotypic tests such as Congo red agar (CRA) and disclosure virulence genes, agglutinin-like sequence(ALS1) and hyphal wall protein (HWP1). The susceptibilities of antifungal medications and plant extracts against C. albicans isolates were then assessed. This study showed low susceptibilities values to all tested antifungals such as econazole (12mm), miconazole (12mm) and nystatin (14mm) in the disc diffusion method with the exception of ketoconazole (20mm) which had high susceptibilities values. In this research each ethanol pomegranates peel extracts (EPPE) (200mg/ml), aquatic pomegranates peel extracts (APPE) (200mg/ml) and pomegranate molasses (PM) were used. In the agar well diffusion method, C. albicans showed low susceptibilities values against (PM) (8mm). However, it showed high susceptibilities values against (EPPE) (18mm) when compared to each of the antifungal agents: econazole, miconazole and nystatin. Results of the study showed that pomegranate peel could be new therapeutic promisingekey ingredient in antifungal medications development. It is an alternate antifungal medication which is useful for C. albicans treatment.

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Antagonistic Activity of Bacteriocin-producing Lactobacillus Against Candida spp

Cited by 9 publications

References 18 publications

Isolation and Identification of Candida dubliniensis in Patient with Cervical-Vulvovaginal Candidiasis in Erbil City

Isolation and Identification of Candida dubliniensis in Patient with Cervical-Vulvovaginal Candidiasis in Erbil City

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

The Efficacy of Antifungal Medications and Plant Extracts Against Candida albicans Isolated from Vulvovaginitis Women

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