Antagonistic activity of bacteria isolated from crops cultivated in a rotation system and a monoculture against Pythium debaryanum and Fusarium oxysporum

Gorlach‐Lira, Krystyna; Stefaniak, O.

doi:10.1007/s12223-009-0062-1

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…Similar effects of plants on the abundance of antagonistic Pseudomonas spp. under monoculture were found by Gorlach-Lira and Stefaniak (2009) . Hence, the abundance of the Pseudomonas populations in soil of R. pseudostellariae were seriously affected by monoculture.…”

Section: Discussionmentioning

confidence: 95%

“…Due to an extensive distribution of Pseudomonas species in the environment, several studies reported an abundance of antagonistic Pseudomonas species, which controls specialized pathogens that are responsible for disease suppression in soils ( Gorlach-Lira and Stefaniak, 2009 ; Mendes et al, 2011 ). Our study of Pseudomonas -DGGE revealed that the diversity of Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of the Diversity of Pseudomonas spp. and Fusarium spp. in Radix pseudostellariae Rhizosphere under Monoculture by Combining DGGE and Quantitative PCR

Chen

Xiao

et al. 2017

Front. Microbiol.

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Radix pseudostellariae is a perennial tonic medicinal plant, with high medicinal value. However, consecutive monoculture of this plant in the same field results in serious decrease in both yield and quality. In this study, a 3-year field experiment was performed to identify the inhibitory effect of growth caused by prolonged monoculture of R. pseudostellariae. DGGE analysis was used to explore the shifts in the structure and diversity of soil Fusarium and Pseudomonas communities along a 3-year gradient of monoculture. The results demonstrated that extended monoculture significantly boosted the diversity of Fusarium spp., but declined Pseudomonas spp. diversity. Quantitative PCR analysis showed a significant increase in Fusarium oxysporum, but a decline in Pseudomonas spp. Furthermore, abundance of antagonistic Pseudomonas spp. possessing antagonistic ability toward F. oxysporum significantly decreased in consecutively monocultured soils. Phenolic acid mixture at the same ratio as detected in soil could boost mycelial and sporular growth of pathogenic F. oxysporum while inhibit the growth of antagonistic Pseudomonas sp. CJ313. Moreover, plant bioassays showed that Pseudomonas sp. CJ313 had a good performance that protected R. pseudostellariae from infection by F. oxysporum. In conclusion, this study demonstrated that extended monoculture of R. pseudostellariae could alter the Fusarium and Pseudomonas communities in the plant rhizosphere, leading to relatively low level of antagonistic microorganisms, but with relatively high level of pathogenic microorganisms.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Assessment of the Diversity of Pseudomonas spp. and Fusarium spp. in Radix pseudostellariae Rhizosphere under Monoculture by Combining DGGE and Quantitative PCR

Chen

Xiao

et al. 2017

Front. Microbiol.

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“…In this study, we also detected differences between treatments with regard to the abundance of bacteria with antagonistic or plant growth promoting capacities. Among the dominant bacteria, Arthrobacter was identified, which is a mycoantagonistic fungi against P. debaryanum and F. oxysporum [50], as well as Streptomyces, which is widely used due to its nitrogen fixing ability, phosphate and potassium solubilization, phytohormone production, and antifungal activity [51]. These fungi were more abundant in soil after faba bean than after wheat cultivation.…”

Section: Discussionmentioning

confidence: 99%

Forecrop Effects on Abundance and Diversity of Soil Microorganisms during the Growth of the Subsequent Crop

2020

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Plant residues introduced into the soil influence the soil’s physical, chemical, and consequently, biological parameters, which have an influence on plant health and yields. This study was undertaken to evaluate the effects of the cultivation of widespread plant species, faba bean (Vicia faba L., F) and wheat (Triticum aestivum L., W) (as forecrops) grown under conventionally tilled fields, on the diversity of microorganisms and the enzymatic activities of soil during the growth of the subsequent wheat. Bulk soil samples (0–20 cm) were taken three times from two months after residue incorporation into the soil to the maturity of the subsequent wheat crop in order to assess the fungal and bacterial communities (Next Generation Sequencing (NGS), terminal restriction fragments lengths polymorphism (t-RFLP)), ammonia oxidizing archaea (t-RFLP, denaturing gradient gel glectrophoresis (DGGE)), and the enzymatic activity of soil. Differences in the genetic structure of ammonia-oxidizing archaea (AOA) were observed for each treatment and sampling term, indicating that plant and weather conditions are the driving force for microorganism selection and adaptation. We observed that the fungal community was more influenced by the forecrop type used than the bacterial community. The activities of the enzymes changed in response to the forecrop and sampling period. A higher number of microorganisms that are associated with plant benefits with respect to nutrients and growth, as well as higher amounts of N in the residues, was noted in faba bean than in wheat soil. This could indicate better growth conditions after faba bean and, consequently, better yield quality and quantity. This may also indicate some protective role of the soil after faba bean against pathogens, which may be connected with lower fungicide requirements. The obtained results lead to a deeper understanding of the microorganism reactions to faba bean and wheat residues during wheat cultivation. In addition, they may be helpful in improving our understanding of subsequent crop yield responses to forecrops.

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“…Different species of Bacillus represented more than half of the identified Fv antagonistic isolates (Additional file 1 : Table S1), as well as the most prominent bacterial populations in this study. Different species belonging to all other genera that showed fungal inhibition in liquid assay (Additional file 1 : Table S1) including Acinetobacter (Magnin-Robert et al 2007 ), Arthrobacter (Cavaglieri et al 2005a ), Enterobacter (van Dijk and Nelson 1997 ; Gopalakrishnan et al 2011 ), Klebsiella (Lynch 1990 ), Lysinibacillus (Trivedi et al 2011 ), Paenibacillus (Nielsen and Sorensen 1997 ), Pantoea (Babalola 2010 ) and Pseudomonas (Gorlach-Lira and Stefaniak 2009 ) are reported to possess antagonistic traits against diverse pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…Different Pseudomonas spp. produce a broad array of lytic enzymes, antibiotics, cyanide, siderophores and antifungal compounds (Nagarajkumar et al 2004 ; Weller et al 2007 ; Gorlach-Lira and Stefaniak 2009 ) which can all inhibit pathogens including Fv .…”

Section: Discussionmentioning

confidence: 99%

Rhizospheric bacteria of maize with potential for biocontrol of Fusarium verticillioides

et al. 2016

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The stalk, ear and root rot (SERR) of maize caused by Fusarium verticillioides (Fv) severely impacts crop production in tropical and subtropical regions. The aim of the present work was to screen bacterial isolates in order to find novel native biocontrol agents against Fv. A culturable bacterial collection consisting of 11,520 isolates enriched in Firmicutes and Proteobacteria was created from rhizosphere samples taken from SERR symptomatic or asymptomatic maize plants. The complete collection was screened for potential activity against Fv using a liquid antagonism assay followed by dual cultures in solid medium, selecting for 42 bacteria (Bacillus, Pseudomonas and Paenibacillus) that inhibit Fv growth (>45 %). In planta assays demonstrated that three Bacillus isolates: B. megaterium (B5), B. cereus sensu lato (B25) and Bacillus sp. (B35) displayed the highest antagonistic activity against Fv. Pot experiments performed in a greenhouse with Bacillus cereus sensu lato B25 confirmed these findings and showed a reduction of Fv disease severity and incidence on plants. Antagonistic activity analysis revealed that these strains produce glucanases, proteases or chitinases, as well as siderophores and auxins and suggests these as possible control mechanisms against Fv.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-1780-x) contains supplementary material, which is available to authorized users.

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Antagonistic activity of bacteria isolated from crops cultivated in a rotation system and a monoculture against Pythium debaryanum and Fusarium oxysporum

Cited by 6 publications

References 18 publications

Assessment of the Diversity of Pseudomonas spp. and Fusarium spp. in Radix pseudostellariae Rhizosphere under Monoculture by Combining DGGE and Quantitative PCR

Assessment of the Diversity of Pseudomonas spp. and Fusarium spp. in Radix pseudostellariae Rhizosphere under Monoculture by Combining DGGE and Quantitative PCR

Forecrop Effects on Abundance and Diversity of Soil Microorganisms during the Growth of the Subsequent Crop

Rhizospheric bacteria of maize with potential for biocontrol of Fusarium verticillioides

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