Antagonistic activity and mechanism of Lactobacillus rhamnosus SQ511 against Salmonella enteritidis

Shi, Shuiqin; Gong, Li; Hao, Yu; He, Guangyu; Zhang, Jingjing; Yu, Han; Liu, Yannan; Hu, Jie; Dong, Jinsheng; Liu, Jia; Zhao, Kai; Zhou, Duoqi

doi:10.1007/s13205-022-03176-5

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…Changes in PI staining, ΔΨ, and ΔpH were consistent with that in E. coli cells treated with a bacteriocin produced by L. rhamnosus 1.0320 [ 32 ]. Additionally, changes in PI staining coincided with that in S. aureus cells conditioned with CFS from L. rhamnosus XN2 [ 21 ], and the depolarization of cell membrane was in agreement with that in S. enteritidis caused by CFS from L. rhamnosus SQ511 [ 20 ]. Besides, disruption of membrane integrity leads to intracellular ATP leakage, which is also responsible for the decreased intracellular ATP concentration [ 33 ].…”

Section: Discussionsupporting

confidence: 54%

“…Moreover, ROS production plays an important role in commencing cessation of the cell during the initiation of cell death [ 24 ]. Here, CFS from L. rhamnosus SCB0119 accumulated the ROS levels in E. coli ATCC25922 and S. aureus ATCC6538, which was similar to the increases of ROS production in S. typhimurium caused by CFS from L. rhamnosus SQ511 [ 20 ]. The high level of ROS production causes oxidative stress, which is closely associated with adverse effects on cellular components and causes cell death [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 69%

“…Additionally, the decreases in intracellular ATP levels suggested that CFS from L. rhamnosus SCB0119 might cause the death of E. coli and S. aureus cells [ 22 ]. The cell death of pathogens caused by L. rhamnosus was also reported in S. typhimurium treated with L. rhamnosus SQ511 [ 20 ].…”

Section: Discussionmentioning

confidence: 86%

“…Previous studies showed that L. rhamnosus could inhibit the growth of several pathogens, such as S. aureus , E. coli , Candida albicans , Shigella sonnei , Salmonella enteritidis , Pseudomonas fluorescens , Pseudomonas putida , and Listeria monocytogene , and its antibacterial activities differed among different L. rhamnosus strains [ 18 , 19 ]. However, it was still little known about the antibacterial mechanisms of L. rhamnosus , though it was reported to inhibit the growth of S. enteritidis by elevating the reactive oxygen species level and destructing bacterial membrane [ 20 ] and inhibiting the growth of S. aureus by increasing cell permeability [ 21 ]. In this study, we evaluated the potential antibacterial effects of L. rhamnosus SCB0119 isolated from fermented pickles against E. coli and S. aureus by investigating the changes in bacterial growth and cell structures.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis Revealed Antimicrobial Mechanisms of Lactobacillus rhamnosus SCB0119 against Escherichia coli and Staphylococcus aureus

Peng

Zhou

Yang

et al. 2022

IJMS

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Lactic acid bacteria were reported as a promising alternative to antibiotics against pathogens. Among them, Lactobacillus rhamnosus could be used as probiotics and inhibit several pathogens, but its antibacterial mechanisms are still less known. Here, L. rhamnosus SCB0119 isolated from fermented pickles could inhibit bacterial growth or even cause cell death in Escherichia coli ATCC25922 and Staphylococcus aureus ATCC6538, which was mainly attributed to the cell-free culture supernatant (CFS). Moreover, CFS induced the accumulation of reactive oxygen species and destroyed the structure of the cell wall and membrane, including the deformation in cell shape and cell wall, the impairment of the integrity of the cell wall and inner membrane, and the increases in outer membrane permeability, the membrane potential, and pH gradient in E. coli and S. aureus. Furthermore, the transcriptomic analysis demonstrated that CFS altered the transcripts of several genes involved in fatty acid degradation, ion transport, and the biosynthesis of amino acids in E. coli, and fatty acid degradation, protein synthesis, DNA replication, and ATP hydrolysis in S. aureus, which are important for bacterial survival and growth. In conclusion, L. rhamnosus SCB0119 and its CFS could be used as a biocontrol agent against E. coli and S. aureus.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptomic Analysis Revealed Antimicrobial Mechanisms of Lactobacillus rhamnosus SCB0119 against Escherichia coli and Staphylococcus aureus

Peng

Zhou

Yang

et al. 2022

IJMS

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“…There are several studies in the literature reporting the antibacterial activity of L. rhamnosus. Shi et al (25) reported significant antibacterial effect of L. rhamnosus postbiotic on S. enteritidis. They also introduced postbiotics as the next possible generation of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antioxidant Properties of Natural Postbiotics Derived from Five Lactic Acid Bacteria

Noori

Behfar

Saadat

et al. 2022

Jundishapur J Nat Pharm Prod

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Background: The application of natural antimicrobial and antioxidant agents in food and pharmaceutical products has recently become a trend due to the high demand for them from consumers. Postbiotics are bioactive compounds that are produced when the healthy bacteria in the gut ferment fiber. Objectives: This study aimed to compare the antibacterial and antioxidant properties of postbiotics from 5 different lactic acid bacteria (LAB) including Lactiplantibacillus fermentum, Lactiplantibacillus plantarum, Lactiplantibacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus. Methods: Two different methods were adopted to obtain postbiotics (M1 and M2). M1 was the simple method in which the centrifugation was employed while in M2 method, ethyl acetate was used to obtain postbiotics. Agar disc diffusion, minimum inhibition concentration, and minimum bactericidal concentration were used to assess the antimicrobial activity of postbiotics. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was performed in order for investigating the antioxidant property. Results: The best results were recorded for L. casei compared to other LABs. Highest values of the agar disc diffusion method were obtained for L. casei. The inhibition zones for Salmonella enterica, Listeria monocytogenes, and Staphylococcus aureus, for examples, were 22 mm, 20 mm, and 19 mm, respectively. The postbiotic of L. casei also exhibited the most potent antioxidant activity among other probiotic bacteria. The data showed that M2 was a more effective method than the other method for acquiring postbiotics. Conclusions: It was recommended LABs postbiotics should be applied as antioxidant, antimicrobial, and preservatives in food and pharmaceutical industries due to their desired effects and natural characteristics.

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Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice

Hu,

Huang,

Shu

et al. 2023

Animals

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Our study aimed to explore the effects of postbiotics on protecting against Salmonella infection in mice and clarify the underlying mechanisms. Eighty 5-week-old C57BL/6 mice were gavaged daily with Lactiplantibacillus plantarum (LP)-derived postbiotics (heat-killed bacteria, LPBinactive; culture supernatant, LPC) or the active bacteria (LPBactive), and gavaged with Salmonella enterica Typhimurium (ST). The Turbidimetry test and agar diffusion assay indicated that LPC directly inhibited Salmonella growth. Real-time PCR and biofilm inhibition assay showed that LPC had a strong ability in suppressing Salmonella pathogenicity by reducing virulence genes (SopE, SopB, InvA, InvF, SipB, HilA, SipA and SopD2), pili genes (FilF, SefA, LpfA, FimF), flagellum genes (FlhD, FliC, FliD) and biofilm formation. LP postbiotics were more effective than LP on attenuating ST-induced intestinal damage in mice, as indicated by increasing villus/crypt ratio and increasing the expression levels of tight junction proteins (Occludin and Claudin-1). Elisa assay showed that LP postbiotics significantly reduced ST-induced inflammation by regulating the levels of inflammatory cytokines (the increased IL-4 and IL-10 and the decreased TNF-α) in serum and ileum (p < 0.05). Furthermore, LP postbiotics inhibited the activation of NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) inflammasome by decreasing the protein expression of NLRP3 and Caspase-1, and the gene expression of Caspase-1, IL-1β and IL-18. Meanwhile, both LPC and LPB observably activated autophagy under ST infection, as indicated by the up-regulated expression of LC3 and Beclin1 and the downregulated p62 level (p < 0.05). Finally, we found that LP postbiotics could trigger an AMP-activated protein kinase (AMPK) signaling pathway to induce autophagy. In summary, Lactiplantibacillus plantarum-derived postbiotics alleviated Salmonella infection via modulating bacterial pathogenicity, autophagy and NLRP3 inflammasome in mice. Our results confirmed the effectiveness of postbiotics agents in the control of Salmonella infection.

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Antagonistic activity and mechanism of Lactobacillus rhamnosus SQ511 against Salmonella enteritidis

Cited by 5 publications

References 37 publications

Transcriptomic Analysis Revealed Antimicrobial Mechanisms of Lactobacillus rhamnosus SCB0119 against Escherichia coli and Staphylococcus aureus

Transcriptomic Analysis Revealed Antimicrobial Mechanisms of Lactobacillus rhamnosus SCB0119 against Escherichia coli and Staphylococcus aureus

Antimicrobial and Antioxidant Properties of Natural Postbiotics Derived from Five Lactic Acid Bacteria

Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice

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