Antagonistic activity and mechanism of a novel Bacillus amyloliquefaciens MB40 strain against fire blight

Shemshura, Olga N.; Alimzhanova, Mereke; Ismailova, Elvira; Molzhigitova, Assel; Daugaliyeva, Saule; Sadanov, A. K.

doi:10.1007/s42161-020-00515-4

Cited by 12 publications

(13 citation statements)

References 39 publications

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“…The strong effect of orchard management on bacterial diversity suggests that application of Bacillus reduced bacterial diversity, which may occur through resource competition, priority effects, or mass effects. Bacillus species have shown promise in limiting the establishment and development of the bacterial pathogen E. amylovora , the causal agent of fire blight (Sundin et al ., 2009; Shemshura et al ., 2020), and may also affect other floral microbes. Indeed, increased fungal diversity in organically-managed orchards could be a consequence of Bacillus application, although we were unable to directly assess if fungal abundance was affected in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus species have shown promise in limiting the establishment and development of the bacterial pathogen E. amylovora, the causal agent of fire blight (Sundin et al, 2009;Shemshura et al, 2020), and may also affect other floral microbes. Indeed, increased fungal diversity in organically-managed orchards could be a consequence of Bacillus application, although we were unable to directly assess if fungal abundance was affected in our study.…”

Section: Discussionmentioning

confidence: 99%

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Orchard Management and Landscape Context Mediate the Floral Microbiome of Pear

Schaeffer¹,

Pfeiffer

Basu

et al. 2020

Preprint

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Crop-associated microbiota are key factors affecting host health and productivity. Most crops are grown within heterogeneous landscapes, and interactions between management practices and landscape context often affect plant and animal biodiversity in agroecosystems. However, whether these same factors typically affect crop-associated microbiota is less clear. Here, we assessed whether orchard management strategies and landscape context affected bacterial and fungal communities in pear (Pyrus communis) flowers. We found that bacteria and fungi responded differently to management schemes. Organically-certified orchards had higher fungal diversity in flowers than conventional or bio-based integrated pest management (IPM) orchards, but organic orchards had the lowest bacterial diversity. Orchard management scheme also best predicted the distribution of several important bacterial and fungal genera that either cause or suppress disease, with organic and bio-based IPM best explaining the distributions of bacterial and fungal genera, respectively. Moreover, patterns of bacterial and fungal diversity were affected by interactions between management, landscape context, and climate. When examining the similarity of bacterial and fungal communities across sites, both abundance- and taxa-related turnover were mediated primarily by orchard management scheme and landscape context, and specifically the amount of land in cultivation. Our study reveals local- and landscape-level drivers of floral microbiome structure in a major fruit crop, providing insights that can inform microbiome management to promote host health and high-yielding quality fruit.IMPORTANCEIn tree fruits, proper crop management during bloom is essential for producing disease-free fruit. Tree fruits are often grown in heterogeneous landscapes; however, few studies have assessed whether landscape context and crop management affect the floral microbiome, which plays a critical role in shaping plant health and disease tolerance. Such work is key for identification of tactics and/or contexts where beneficial microbes proliferate, and pathogenic microbes are limited. Here, we characterize the floral microbiome of pear crops in Washington State, USA, where major production occurs in inter-mountain valleys and basins with variable elevation and microclimates. Our results show that both local (crop management) and landscape (habitat types and climate) level factors affect floral microbiota, but in disparate ways for each kingdom, suggesting a need for unique management strategies for each group. More broadly, these findings can potentially inform microbiome management in orchards for promotion of host health and high-quality yields.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Orchard Management and Landscape Context Mediate the Floral Microbiome of Pear

Schaeffer¹,

Pfeiffer

Basu

et al. 2020

Preprint

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“…Inhibition zone assays were performed to screen for antagonistic bacteria against E. amylovora (Shemshura et al, 2020). Culture plates with the media were pre-plated with E. amylovora TS 3128 strain (Kang et al, 2021) 12 hr before spot inoculation of the isolated colonies, followed by incubation at 28°C for 3 days.…”

Section: Methodsmentioning

confidence: 99%

“…The amplicons were eluted using 1% agarose gel electrophoresis and were cloned into the TOPO TA cloning vector (Invitrogen, Carlsbad, CA, USA), and sequenced. Each sequence was compared using BLAST to identify the species and MEGA XI with the neighbor-joining method with 1,000 bootstrap replicates was used to construct the phylogenetic trees (Shemshura et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…P. protegens 59M (Mikiciński et al, 2020); the genera Pantoea: P. agglomerans E325 (Pusey, 2002) and P10c (Vanneste et al, 2002), P. vagans C9-1 (Ishimaru et al, 1988;Smits et al, 2010); the genera Bacillus: B. subtilis QST713 (Aldwinckle et al, 2002) and BD170 (Broggini et al, 2005). B. amyloliquefaciens LMR2 (Bahadou et al, 2018), MB40 (Shemshura et al, 2020), and FZB42 (Chen et al, 2009); Paenibacillus polymixa N179 (Fallahzadeh-Mamaghani et al, 2021); Lactobacillus plantarum (Roselló et al, 2013); Enterobacter sp. En113 and Serratia sp.…”

Section: Introductionmentioning

confidence: 99%

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Screening of Bacterial Antagonists to Develop an Effective Cocktail against Erwinia amylovora

Choi

Kim

et al. 2022

Res. Plant Dis

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Several types of chemical bactericides have been used to control fire blight. However, their excessive usage leads to environmental deterioration. Therefore, several researchers have analyzed antagonistic microorganisms as promising, effective, and safe biological control agents (BCAs). The primary aim of this study was to screen for potential antagonistic bacteria that suppress Erwinia amylovora. Among the 45 isolates studied, 5 strains showed the largest inhibition zone against E. amylovora. 16S rRNA gene sequencing identified them as Bacillus amyloliquefaciens (KPB 15), B. stratosphericus (KPB 21), B. altitudinis (KPB 25), B. safensis (KPB 31), and B. subtilis (KPB 39). KPB 25 and 31 reduced the lesion size of fire blight by 50% in immature apple fruits, and did not show antagonism against each other. Therefore, KPB 25 and 31 were selected to develop an antagonistic mixture against fire blight. Although the mixture with KPB 25 and 31 showed a slightly increased ability to reduce lesion size on immature fruits, they did not exhibit a synergistic effect in reducing E. amylovora population compared to each strain alone. Nevertheless, we have identified these two strains as useful and novel BCAs against fire blight with additional benefits safety and potential in developing a mixture without loss of their activity, owing to the absence of antagonism against each other.

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