Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in
            <i>Drosophila</i>

Liu, Suning; Li, Kang; Gao, Yue; Liu, Xi; Chen, Weiting; Ge, Wei; Feng, Qiang; Palli, Subba Reddy; Li, Sheng

doi:10.1073/pnas.1716897115

Cited by 160 publications

(139 citation statements)

References 55 publications

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“…Recent reports indicate that JH can inhibit ecdysone production in holometabolan insects, such as the fly D. melanogaster and the silkmoth Bombyx mori. The effect is mediated by Kr-h1, which represses the expression of ecdysteroidogenic genes (37,38). Therefore, it is plausible that the reduced expression of nvd, phm, and dib in dsMyo-treated cockroaches could be due to an increase in Kr-h1 expression resulting from Myo depletion ( Fig.…”

Section: Myo Depletion Prevents the Onset Of Pg Degenerationmentioning

confidence: 99%

“…This supports the hypothesis that FTZ-F1 helps enhance phm and dib transcription in the PG of B. germanica. Finally, decreased expression of ecdysteroidogenic genes in Myodepleted insects might be mediated by the increased expression of jhamt and Kr-h1 because JH and Kr-h1 inhibit the expression of these genes (37,38). We explored this possibility through experiments examining Myo in N6.…”

Section: Myo Depletion Reduces the Expression Of Ecdysteroidogenic Gementioning

confidence: 99%

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Myoglianin triggers the premetamorphosis stage in hemimetabolan insects

Kamsoi

Bellés

2018

FASEB j.

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Insect metamorphosis is triggered by a decrease in juvenile hormone (JH) in the final juvenile instar. What induces this decrease is therefore a relevant question. Working with the cockroach Blattella germanica, we found that myoglianin (Myo), a ligand in the TGF‐β signaling pathway, is highly expressed in the corpora allata (CA, the JH‐producing glands) and the prothoracic gland [(PG), which produce ecdysone] during the penultimate (fifth) nymphal instar (N5). In the CA, high Myo levels during N5 repress the expression of juvenile hormone acid methyl transferase, a JH biosynthesis gene. In the PG, decreasing JH levels trigger gland degeneration, regulated by the factors Krüppel homolog 1, FTZ‐F1, E93, and inhibitor of apoptosis‐1. Also in the PG, a peak of myo expression in N5 indirectly stimulates the expression of ecdysone biosynthesis genes, such as neverland, enhancing the production of the metamorphic ecdysone pulse in N6. The Myo expression peak in N5 also represses cell proliferation, which can enhance ecdysone production. The data indicate that Myo triggers the premetamorphic nymphal instar in B. germanica and possibly in other hemimetabolan insects.—Kamsoi, O., Belles, X. Myoglianin triggers the premetamorphosis stage in hemimetabolan insects. FASEB J. 33, 3659–3669 (2019). http://www.fasebj.org

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Section: Myo Depletion Prevents the Onset Of Pg Degenerationmentioning

confidence: 99%

Section: Myo Depletion Reduces the Expression Of Ecdysteroidogenic Gementioning

confidence: 99%

Myoglianin triggers the premetamorphosis stage in hemimetabolan insects

Kamsoi

Bellés

2018

FASEB j.

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“…Hence, JH suppresses ecdysone biosynthesis and inhibits metamorphosis, whereas 20E suppresses JH biosynthesis and promotes metamorphosis. Antagonistic actions of JH and 20E at the hormone‐biosynthesis levels determine developmental transitions in D. melanogaster (Liu et al ., ; Zhang et al ., ).…”

Section: The Anti‐metamorphic Role Of Kr‐h1 and Kr‐h1 Binding Sitementioning

confidence: 99%

“…Thus, JH prevents 20E‐induced metamorphosis and is therefore referred to as the status quo hormone. During middle and late juvenile (larval or nymphal) stages, JH coordinates with 20E to maintain the juvenile status by preventing metamorphosis; in the final juvenile instar, insects undergo pupation or adult morphogenesis because of a sharp decrease in JH titer (Jindra et al ., ; Belles & Santos, ; Jindra et al ., ; Liu et al ., ). In adults, JH participates in regulating reproduction, including previtellogenic development, vitellogenesis and oogenesis (Roy et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Juvenile hormone signaling – a mini review

Jia

2018

Insect Science

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113

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Since it was first postulated by Wigglesworth in 1934, juvenile hormone (JH) is considered a status quo hormone in insects because it prevents metamorphosis that is initiated by the molting hormone 20-hydroxyecdysone (20E). During the last decade, significant advances have been made regarding JH signaling. First, the bHLH-PAS transcription factor Met/Gce was identified as the JH intracellular receptor. In the presence of JH, with the assistance of Hsp83, and through physical association with a bHLH-PAS transcriptional co-activator, Met/Gce enters the nucleus and binds to E-box-like motifs in promoter regions of JH primary-response genes for inducing gene expression. Second, the zinc finger transcription factor Kr-h1 was identified as the anti-metamorphic factor which transduces JH signaling. Via Kr-h1 binding sites, Kr-h1 represses expression of 20E primary-response genes (i.e. Br, E93 and E75) to prevent 20E-induced metamorphosis. Third, through the intracellular signaling, JH promotes different aspects of female reproduction. Nevertheless, this action varies greatly from species to species. Last, a hypothetical JH membrane receptor has been predicted to be either a GPCR or a tyrosine kinase receptor. In future, it will be a great challenge to understand how the JH intracellular receptor Met/Gce and the yet unidentified JH membrane receptor coordinate to regulate metamorphosis and reproduction in insects.

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“…In the presence of JH, 20E cannot trigger pupation but ecdysis to another larva Liu et al, 2018). In the absence of JH, 20E initiates the pupal programme, in which larval structures are transformed into imaginal ones and new pupal cuticle is deposited.…”

Section: Introductionmentioning

confidence: 99%

Isoform specific roles of Broad‐Complex in larval development in Leptinotarsa decemlineata

Meng

Deng

et al. 2019

Insect Molecular Biology

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Broad‐Complex (BrC) is a downstream target of both 20‐hydroxyecdysone and juvenile hormone signalling. BrC regulates morphogenetic changes between nymphal instars in hemimetabolans, whereas it controls pupal commitment, pupal morphogenesis and inhibits adult differentiation in holometabolans. Among five BrC cDNAs (Z1–Z4 and Z6) identified in the Colorado potato beetle, we found in this work that Z1, Z2 and Z6 were mainly expressed at the last (fourth) instar and prepupal stages, whereas the levels of Z3 and Z4 increased during the penultimate (third) instar stage, peaked at the last instar larval phase and gradually decreased at the prepupal and pupal periods. When knocking down all BrC isoforms by RNA interference (RNAi) at the penultimate instar stage, around 20% of the resultant larvae remained as moribund beetles. These moribund BrC RNAi larvae were completely or partially wrapped in old cuticle. Likewise, a portion of larvae treated for a single double‐stranded RNA of Z3, Z4 or Z6 displayed a degree of similar aberrancies, increasing in the order of isoforms Z6 < Z3 < Z4. When silencing all BrC isoforms at the last instar period, most of the RNAi larvae did not normally pupate or emerge as adults. Separately silencing each of the five zinc finger domains revealed that approximately 70% of the Z1 RNAi larvae remained as prepupae, around 60% of the Z6 RNAi specimens formed aberrant prepupae or pupae and about 60% of the Z2 RNAi beetles became deformed pupae. After removal of the old exuviae, these deformed larvae in which either Z1, Z2 or Z6 was depleted possessed adult prothorax and mesothorax, developing antenna, mouthparts and wing discs. Moreover, less than 50% of the resultant pupae finally emerged as adults when either of Z1, Z2 or Z6 was knocked down. Therefore, our findings reveal, for the first time, that the two roles of BrC in insect groups (ie directing morphogenetic changes during juvenile development and regulating larval–pupal–adult metamorphosis) are played by different BrC isoforms in Leptinotarsa decemlineata.

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Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila

Cited by 160 publications

References 55 publications

Myoglianin triggers the premetamorphosis stage in hemimetabolan insects

Myoglianin triggers the premetamorphosis stage in hemimetabolan insects

Juvenile hormone signaling – a mini review

Isoform specific roles of Broad‐Complex in larval development in Leptinotarsa decemlineata

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