Antagonistic action of Streptomyces pratensis S10 on Fusarium graminearum and its complete genome sequence

Abstract

Wheatscab, mainly caused by Fusarium graminearum, can decrease wheat yield and grain quality. Chemical pesticides are currently the main control method but have an inevitable negative consequence on the environment and in food safety. This research studies a promising substitute, Streptomyces pratensis S10, which was isolated from tomato leaf mould and shows a significant inhibition effect on F. graminearum based on antagonism assays. The biocontrol mechanism is studied by enhanced green fluorescent protein labelling, quantitative real-time PCR, the Doskochilova 8 solvents system test and complete genome sequencing. Strain S10 can colonize in the wheat root, control wheat scab and decrease deoxynivalenol (DON) content. The control effects in vitro, planta and the plot experiments were 92.86%, 68.67% and 40.87% to 86.62%, respectively. S10 decreased DON content by inhibiting the mycelium growth and DON synthesis gene expression. The active substances of the S10 secondary metabolites had a high-temperature resistance and 29 putative biosynthetic gene clusters in its genome. The S10 control mechanism is multivariate, which shows potential in controlling wheat scab.

Wheat Seed Coating with Streptomyces sp. Strain DEF39 Spores Protects against Fusarium Head Blight

“…This phenomenon has already been demonstrated in the interaction of streptomycetes on maize infected by Fusarium verticillioides [32]. The complexity of the interactions occurring between streptomycetes and wheat plant is likely multivariate [30].…”

“…Further studies can focus on the complex mechanism of protection that DEF39 exploits while interacting with wheat and F. graminearum. The ability of Streptomyces species to synthesize plant-protective molecules including enzymes, secondary metabolites, and volatile organic compounds, as well as their ability to induce the plant immune system to respond quickly to infections, is what makes them potentially valuable biocontrol agents [9,29,30]. Moreover, streptomycetes have the advantage of not only being a potentially co-evolving force capable of engaging in an arms race with pathogenic species, but many also encode numerous putative antimicrobial biosynthetic gene clusters (BGCs), resulting in the simultaneous production of a plethora of different antibiotics with various modes of action [31].…”

Arms Race between the Host and Pathogen Associated with Fusarium Head Blight of Wheat

“…from Streptomyces Aureus have a strong inhibitory effect on wheat FHB [ 154 ]. Zhang et al (2020) found that Streptomyces pratensis strain S10 parasitized wheat roots and inhibited wheat FHB by inhibiting mycelial growth and reducing DON gene expression [ 155 ]. Moreover, Frenolicin B, the main active component from fermentation broth of Streptomyces sp.…”

The Biocontrol and Plant Growth-Promoting Properties of Streptomyces alfalfae XN-04 Revealed by Functional and Genomic Analysis

“…Creation of the Strain XN-04 Labeled With EGFP Escherichia coli strain ET12567 (harboring the helper plasmid pUZ8002) and plasmid pIJ8641 (carrying the EGFP gene under the constitutive ermE promoter) were preserved in the Biological Control of Plant Disease Laboratory. Plasmid pIJ8641 was transformed into the donor strain E. coli ET12567 (pUZ8002) and then conjugated into recipient XN-04 as previously described (Kieser et al, 2000;Zhang et al, 2021). Genomic DNA of wild-type (WT-) and transformed (t-) XN-04 was extracted, and the quality was tested by primers 27F (5′-AGAGT TTGAT CCTGG CTCAG-3′) and 1492R (5′-GGTTA CCTTG TTACG ACTT-3′; Huang et al, 2020).…”