Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm

Rasul, Zahid; Ahmed, N.; Anzar, M.

doi:10.1016/j.theriogenology.2007.06.014

Cited by 48 publications

(37 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Esta ferramenta não somente pode ser utilizada para diminuir ou retirar a subjetividade do método convencional de avaliação da motilidade, mas também para investigar a cinética detalhada dos espermatozoides (MANDAL; BAGPAUL; GUPTA, 2003). Poucos estudos foram realizados com sêmen bubalino com o propósito de avaliar o comportamento da cinética espermática após os processos de congelação e descongelação (RASUL; AHMAD; ANZAR, 2000; RASUL; AHMAD; ANZAR, 2007;KOONJAENAK et al, 2007;ANIL KUMAR et al, 2011).…”

Section: Introductionunclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

View full text Add to dashboard Cite

This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

show abstract

Section: Introductionunclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Similarly, the use of DMSO (6 %) added in one-step, lowered the progressive motility of Angora buck spermatozoa at postthawing (Büyükleblebici et al 2014). In contrast, Rasul et al (2007) could not find the synergism between glycerol and DMSO in improving the progressive motility of buffalo spermatozoa during cryopreservation. The addition of DMSO alone or in combination with glycerol proved to be toxic for buffalo spermatozoa rather than creating the osmotic shock.…”

Section: Discussionmentioning

confidence: 66%

“…The addition of DMSO alone or in combination with glycerol proved to be toxic for buffalo spermatozoa rather than creating the osmotic shock. Moreover, the absence of glycerol in the extender led to a complete cessation of sperm movement, despite negligible protection to plasma membrane and acrosome (Rasul et al 2007). The toxicity of DMSO at certain concentration and temperature could be due to its ability to induce nonlamellar structures in phospholipids and to enhance membrane permeability that might compromise the sperm structures and functions (Sum and de Pablo 2003).…”

Section: Discussionmentioning

confidence: 97%

“…Moreover, studies on cattle bull (Snedeker and Gaunya 1970) and rabbit buck (Bamba and Adams 1990) spermatozoa have revealed that DMSO in combination with glycerol is superior in providing cryoprotection. Conversely, DMSO alone (1.5 or 3 %) or in combination with glycerol (1.5 or 3 % DMSO and 3 or 6 % glycerol) added either at 4 or 37°C did not provide cryoprotection to buffalo bull spermatozoa (Rasul et al 2007). The harmful effect of DMSO during cryopreservation is linked with its toxicity rather than the osmotic shock (Rasul et al 2007).…”

Section: Introductionmentioning

confidence: 96%

“…Conversely, DMSO alone (1.5 or 3 %) or in combination with glycerol (1.5 or 3 % DMSO and 3 or 6 % glycerol) added either at 4 or 37°C did not provide cryoprotection to buffalo bull spermatozoa (Rasul et al 2007). The harmful effect of DMSO during cryopreservation is linked with its toxicity rather than the osmotic shock (Rasul et al 2007). Therefore, there is a possibility to overcome these effects by testing new concentrations of DMSO and glycerol in extender for cryopreservation of buffalo spermatozoa.…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

Shah

Andrabi²,

Ahmed³

et al. 2016

Cytotechnology

View full text Add to dashboard Cite

The objective of the study was to devise a cryoprotection synergism between glycerol and dimethyl sulfoxide (DMSO) for water buffalo spermatozoa. Additionally, the effect of best evolved concentrations of glycerol and DMSO in extender was assessed on in vivo fertility of buffalo spermatozoa. Ejaculates (n = 30) were equally distributed into five aliquots; first aliquot was diluted at 37°C in extender having 7 % glycerol (control); second aliquot was diluted at 37°C as well as at 4°C in extender having 3.5 % DMSO (Group 1); third aliquot was diluted at 37°C in extender having 3.5 % glycerol and then at 4°C in extender having 3.5 % DMSO (Group 2); fourth aliquot was diluted at 37°C in extender having 3.5 % DMSO and then at 4°C in extender having 3.5 % glycerol (Group 3); fifth aliquot was diluted in extenders having 1.75 % glycerol and 1.75 % DMSO at 37 as well as at 4°C (Group 4). At post thawing, sperm progressive motility (%), rapid velocity (%), average path velocity (lm/s), curved line velocity (lm/s), in vitro longevity (%), structural and functional integrity of plasmalemma (%), mitochondrial transmembrane potential (%) and viable sperm with intact acrosome (%) were higher (P \ 0.05) in Group 4 compared to other treatment groups and control. Regarding sperm DNA integrity (%); it was higher (P \ 0.05) in Group 4 compared to Group 1, 3 and control. The in vivo fertility (%) of buffalo spermatozoa was significantly higher with Group 4 compared to control (69.45 vs. 59.81). In conclusion, synergism exists between glycerol and DMSO (Group 4) in improving the quality and in vivo fertility of cryopreserved water buffalo spermatozoa.

show abstract

Advances in Cryopreservation of Buffalo Semen

Vale¹,

Castro

Almeida-Silva³

et al. 2022

Biotechnological Applications in Buffalo Research

View full text Add to dashboard Cite

Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm

Cited by 48 publications

References 34 publications

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

Advances in Cryopreservation of Buffalo Semen

Contact Info

Product

Resources

About