Antagonism between GLD-2 Binding Partners Controls Gamete Sex

Kim, Kyung Won; Nykamp, Keith; Suh, Nayoung; Bachorik, Jennifer L.; Wang, Liaoteng; Kimble, Judith

doi:10.1016/j.devcel.2009.04.002

Cited by 51 publications

(91 citation statements)

References 46 publications

(71 reference statements)

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“…8.4 ). Further, GLD-3, RNP-8, and FBF stimulate GLD-2 poly(A) polymerase activity in vitro (Wang et al 2002 ;Kim et al 2009a ) . Thus, given their spatially distinct expression patterns it is likely that different cytoPAP complexes are formed, activating translation of speci fi c mRNA targets at speci fi c germline stages (Fig.…”

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

“…8.4 ): DAZ-1, a conserved RRMcontaining protein, follows a type A pattern, CPB-3 fi ts a type B pattern, and the RRM protein RNP-8 fi ts an extended modi fi ed type D pattern, as it is abundant in pachytene (Hasegawa et al 2006 ;Maruyama et al 2005 ;Kim et al 2009a ) . The multi-KH domain protein GLD-3 expression is similar to a type C pattern (Eckmann et al 2002 ) .…”

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

“…A GLD-2/GLD-3 complex may be important for meiotic progression. GLD-3 and RNP-8 may control GLD-2 activity separately to promote the sperm or oocyte fate, respectively (Kim et al 2009a ) , but together they may regulate maternal mRNA storage during oocyte maturation . A peculiarity of the GLD-2 expression pattern is its low abundance in the distal-most mitotic region, which may re fl ect a lower demand for GLD-2 in mitotic cells and/or that mitotic cells regulate GLD-2 in a speci fi c manner.…”

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

“…The multi-KH domain protein GLD-3 expression is similar to a type C pattern (Eckmann et al 2002 ) . GLD-3, and RNP-8 are protein interactors of the ubiquitously expressed cytoPAP GLD-2 (Kim et al 2009a ;Wang et al 2002 ) (Fig. 8.4 ).…”

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

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Translational Control in the Caenorhabditis elegans Germ Line

Nousch

Eckmann

2012

Advances in Experimental Medicine and Biology

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Translational control is a prevalent form of gene expression regulation in the Caenorhabditis elegans germ line. Linking the amount of protein synthesis to mRNA quantity and translational accessibility in the cell cytoplasm provides unique advantages over DNA-based controls for developing germ cells. This mode of gene expression is especially exploited in germ cell fate decisions and during oogenesis, when the developing oocytes stockpile hundreds of different mRNAs required for early embryogenesis. Consequently, a dense web of RNA regulators, consisting of diverse RNA-binding proteins and RNA-modifying enzymes, control the translatability of entire mRNA expression programs. These RNA regulatory networks are tightly coupled to germ cell developmental progression and are themselves under translational control. The underlying molecular mechanisms and RNA codes embedded in the mRNA molecules are beginning to be understood. Hence, the C. elegans germ line offers fertile grounds for discovering post-transcriptional mRNA regulatory mechanisms and emerges as great model for a systems level understanding of translational control during development.

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Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

See 2 more Smart Citations

Translational Control in the Caenorhabditis elegans Germ Line

Nousch

Eckmann

2012

Advances in Experimental Medicine and Biology

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“…For example, analyses of post-transcriptional controls during gametogenesis in Caenorhabditis elegans have identified three factors, GLD-3, RNP-8, and GLS-1, that recruit cytoplasmic polyadenylation to subsets of mRNAs (Wang et al 2002;Kim et al 2009;Schmid et al 2009). Each factor is likely to recognize distinct sets of cis elements, and each is nonorthologous with CPEB.…”

Section: Introductionmentioning

confidence: 99%

The poly(rC)-binding protein αCP2 is a noncanonical factor in X. laevis cytoplasmic polyadenylation

Vishnu

Sumaroka²,

Klein³

et al. 2011

RNA

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Post-transcriptional control of mRNA stability and translation is central to multiple developmental pathways. This control can be linked to cytoplasmic polyadenylation in certain settings. In maturing Xenopus oocytes, specific mRNAs are targeted for polyadenylation via recruitment of the Cytoplasmic Polyadenylation Element (CPE) binding protein (CPEB) to CPE(s) within the 39 UTR. Cytoplasmic polyadenylation is also critical to early embryonic events, although corresponding determinants are less defined. Here, we demonstrate that the Xenopus ortholog of the poly(rC) binding protein aCP2 can recruit cytoplasmic poly(A) polymerase activity to mRNAs in Xenopus post-fertilization embryos, and that this recruitment relies on cis sequences recognized by aCP2. We find that the ha-globin 39 UTR, a validated mammalian aCP2 target, constitutes an effective target for cytoplasmic polyadenylation in Xenopus embryos, but not during Xenopus oocyte maturation. We further demonstrate that the cytoplasmic polyadenylation activity is dependent on the action of the C-rich aCP-binding site in conjunction with the adjacent AAUAAA. Consistent with its ability to target mRNA for poly(A) addition, we find that XaCP2 associates with core components of the Xenopus cytoplasmic polyadenylation complex, including the cytoplasmic poly(A) polymerase XGLD2. Furthermore, we observe that the C-rich aCP-binding site can robustly enhance the activity of a weak canonical oocyte maturation CPE in early embryos, possibly via a direct interaction between XaCP2 and CPEB1. These studies establish XaCP2 as a novel cytoplasmic polyadenylation trans factor, indicate that C-rich sequences can function as noncanonical cytoplasmic polyadenylation elements, and expand our understanding of the complexities underlying cytoplasmic polyadenylation in specific developmental settings.

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Caenorhabditis elegans DLC‐1 associates with ribonucleoprotein complexes to promote mRNA regulation

2018

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Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previously identified as a cofactor of two unrelated RNA-binding proteins that act in the C. elegans germline. Among the 2732 potential DLC-1 targets, most are germline mRNAs associated with oogenesis. Removal of DLC-1 affects expression of its targets expressed in the oocytes, meg-1 and meg-3. We propose that DLC-1 acts as a cofactor for multiple ribonucleoprotein complexes, including the ones regulating gene expression during oogenesis.

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Antagonism between GLD-2 Binding Partners Controls Gamete Sex

Cited by 51 publications

References 46 publications

Translational Control in the Caenorhabditis elegans Germ Line

Translational Control in the Caenorhabditis elegans Germ Line

The poly(rC)-binding protein αCP2 is a noncanonical factor in X. laevis cytoplasmic polyadenylation

Caenorhabditis elegans DLC‐1 associates with ribonucleoprotein complexes to promote mRNA regulation

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