Antagonism between abscisic acid and gibberellin regulates starch synthesis and corm development in Gladiolus hybridus

Li, Jingru; Seng, Shanshan; Li, Donglei; Zhang, Fengqin; Liu, Yixuan; Yao, Ting; Liang, Jiahui; Yi, Mingfang; Wu, Jian

doi:10.1038/s41438-021-00589-w

Cited by 20 publications

(13 citation statements)

References 76 publications

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“…In sweet potato, whose agronomical profitability depends on its ability to transform adventitious roots in storage organs, the addition of GA promotes root lignification and reduces starch accumulation, thus preventing the shift from root to storage-root organ [ 77 ]. Similarly, in Gladiolus hybridus , GA prevents starch synthesis and corm development via GhSUS2 activation [ 78 ]. On the other hand, in Panax ginseng and yam, GA promotes secondary growth of storage roots [ 79 , 80 ].…”

Section: Vegetative Developmentmentioning

confidence: 99%

Plant Development and Crop Yield: The Role of Gibberellins

Castro-Camba

Sánchez²,

Vidal³

et al. 2022

Plants

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Gibberellins have been classically related to a few key developmental processes, thus being essential for the accurate unfolding of plant genetic programs. After more than a century of research, over one hundred different gibberellins have been described. There is a continuously increasing interest in gibberellins research because of their relevant role in the so-called "Green Revolution", as well as their current and possible applications in crop improvement. The functions attributed to gibberellins have been traditionally restricted to the regulation of plant stature, seed germination, and flowering. Nonetheless, research in the last years has shown that these functions extend to many other relevant processes. In this review, the current knowledge on gibberellins homeostasis and mode of action is briefly outlined, while specific attention is focused on the many different responses in which gibberellins take part. Thus, those genes and proteins identified as being involved in the regulation of gibberellin responses in model and non-model species are highlighted. The present review aims to provide a comprehensive picture of the state-of-the-art perception of gibberellins molecular biology and its effects on plant development. This picture might be helpful to enhance our current understanding of gibberellins biology and provide the know-how for the development of more accurate research and breeding programs.

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Section: Vegetative Developmentmentioning

confidence: 99%

Plant Development and Crop Yield: The Role of Gibberellins

Castro-Camba

Sánchez²,

Vidal³

et al. 2022

Plants

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“…In this study, DEGs were enriched in ABA-associated pathways in the GO and KEGG analyses, while the ABA content changed under drought stress, which demonstrated that ABA-associated pathways participate in responding to drought stress in tiger nuts. The starch and sucrose metabolism pathway is closely associated with ABA accumulation [ 62 , 63 , 64 ], which was also associated with responses to drought stress [ 36 ]. Starch is made in chloroplasts in leaves which the content decreased under drought stress [ 65 ], while sucrose is produced in leaves following photosynthesis, which the content increased to balance osmotic pressure within plant cells under drought stress [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis Demonstrates Different Strategies Employed by Tiger Nuts (Cyperus esculentus L.) in Response to Drought Stress

Wei

Liu

et al. 2022

Life

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Drought stress, an important abiotic stress, has affected global agricultural production by limiting the yield and the quality of crops. Tiger nuts (Cyperus esculentus L.) are C4 crops in the Cyperaceae family, which have high-quality wholesome ingredients. However, data on mechanisms underlying the response of tiger nuts to drought stress are few. Here, the variety of Jisha 1 and 15% polyethylene glycol (PEG; a drought stress simulator) were used to study the mechanisms of stress response in tiger nuts. Our evaluation of the changes in physiological indicators such as electrolyte leakage (El), malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide anion (O2−) and activities of reactive oxygen species (ROS) showed that 12 h was the most suitable time point to harvest and analyze the response to drought stress. Thereafter, we performed transcriptome (RNA-Seq) analysis in the control (CK) and stress treatment groups and showed that there was a total of 1760 differentially expressed genes (DEGs). Gene Ontology (GO) analysis showed that the DEGs were enriched in abscisic acid (ABA) terms, and pathways such as starch and sucrose metabolism (ko00500), phenylpropanoid biosynthesis (ko00940) and plant hormone signal transduction (ko04075) were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In addition, quantitative real-time PCR (qRT-PCR) analysis of the DEGs demonstrated an upregulation of ABA and lignin content, as well as enzyme activities in enriched pathways, which validated the RNA-Seq data. These results revealed the pathways and mechanisms adopted by the tiger nuts in response to drought stress.

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“…Silencing of the targeted gene ( LhMYB114 , LhANS-rr1 , or LhDFR ) by VIGS was performed as described earlier [ 37 , 38 ]. According to the experiment protocol, a 200–500 base pair (bp) fragment, specific to the target gene, was designed and cloned into the pTRV2 vector (primers are listed in Table S1 ) using a One-step Cloning Kit (Vazyme, Nanjing, China), which was named 35S:LhMYB115, 35S:LhANS-rr1, and 35S:LhDFR.…”

Section: Methodsmentioning

confidence: 99%

LhANS-rr1, LhDFR, and LhMYB114 Regulate Anthocyanin Biosynthesis in Flower Buds of Lilium ‘Siberia’

Shaozhong

Lin

Ali

et al. 2023

Genes

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The bulb formation of Lilium is affected by many physiological and biochemical phenomena, including flower bud differentiation, starch and sucrose accumulation, photoperiod, carbon fixation, plant hormone transduction, etc. The transcriptome analysis of flower buds of Lilium hybrid ‘Siberia’ at different maturity stages showed that floral bud formation is associated with the accumulation of anthocyanins. The results of HPLC-MS showed that cyanidin is the major anthocyanin found in Lilium ‘Siberia’. Transcriptome KEGG enrichment analysis and qRT-PCR validation showed that two genes related to flavonoid biosynthesis (LhANS-rr1 and LhDFR) were significantly up-regulated. The functional analysis of differential genes revealed that LhMYB114 was directly related to anthocyanin accumulation among 19 MYB transcription factors. Furthermore, the qRT-PCR results suggested that their expression patterns were very similar at different developmental stages of the lily bulbs. Virus-induced gene silencing (VIGS) revealed that down-regulation of LhANS-rr1, LhDFR, and LhMYB114 could directly lead to a decrease in anthocyanin accumulation, turning the purple phenotype into a white color. Moreover, this is the first report to reveal that LhMYB114 can regulate anthocyanin accumulation at the mature stage of lily bulbs. The accumulation of anthocyanins is an important sign of lily maturity. Therefore, these findings have laid a solid theoretical foundation for further discussion on lily bulb development in the future.

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Antagonism between abscisic acid and gibberellin regulates starch synthesis and corm development in Gladiolus hybridus

Cited by 20 publications

References 76 publications

Plant Development and Crop Yield: The Role of Gibberellins

Plant Development and Crop Yield: The Role of Gibberellins

RNA-Seq Analysis Demonstrates Different Strategies Employed by Tiger Nuts (Cyperus esculentus L.) in Response to Drought Stress

LhANS-rr1, LhDFR, and LhMYB114 Regulate Anthocyanin Biosynthesis in Flower Buds of Lilium ‘Siberia’

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