AntagomiR-27a Targets FOXO3a in Glioblastoma and Suppresses U87 Cell Growth in Vitro and in Vivo

Abstract

Objective: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. Methods: The miR27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth. Results: qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor. Conclusion: MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.

MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28

“…Interestingly, introduction of miR-23a antisense into 5-FU resistant cells showed an increased level of apoptotic protease activating facter-1 (APAF-1), along with an enhanced activation of caspase-3 and -7, subsequently enhanced the 5-FU induced apoptosis in these cells [40]. By harnessing a similar strategy, Ge et al investigated the effect of antagomiR-27a in glioblastoma [41]. As aberrant miR-21 and miR-592 seen in CRC, more abundant miR-27a transcript was detected in specimens from glioblastoma comparing with normal human brain tissues.…”

Curcumin Enhances the Radiosensitivity of U87 Cells by Inducing DUSP-2 Up-Regulation

“…For patients with tumors invading important functional areas or patients whose systemic state does not allow surgery, radiation therapy can be used as the preferred method of treatment [1]. However, due to the severe cell hypoxia phenomenon that occurs during radiation therapy, malignant gliomas inherently show poor response to radiotherapy alone and the relapse rate is still very high.…”

Association of a Pre-miR-27a Polymorphism with Cancer Risk: an Updated Meta-analysis

“…The oncogenic miR-27 is known to regulate components involved in numerous types of cancer, including breast cancer (Mertens-Talcott et al, 2007), hepatocellular carcinoma (Wu et al, 2013), glioblastoma (Ge et al, 2013) and gastric cancer (Yang et al, 2014). Genetic variant in pre-miR-27a (rs895819) has been demonstrated to be associated with breast cancer risk and this association was mainly observed in the breast cancers with age of less than 50-year old group or in bilateral breast cancer group (Yang et al, 2010); however, later studies showed conflicting associations (Catucci et al, 2012;Zhang et al, 2012).…”